Enzyme-driven bottle-shaped nano-motor and preparation method thereof

A bottle-shaped, nano-technology, applied in the field of medical biomaterials, can solve the problems of poor biological practicability, difficult positioning and control, and toxic and side effects of bubble-driven motors, and achieve strong controllability, good biocompatibility, and simple preparation process Effect

Active Publication Date: 2018-09-14
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low content of hydrogen peroxide in the human body and certain toxic and side effects on the human body, the biological practicability of the bubble-driven motor is poor, and the controllability of the external field-driven motor is poor, and it is difficult to locate and control its movement

Method used

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  • Enzyme-driven bottle-shaped nano-motor and preparation method thereof
  • Enzyme-driven bottle-shaped nano-motor and preparation method thereof
  • Enzyme-driven bottle-shaped nano-motor and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] See figure 1 , figure 2 , image 3 ,

[0038] figure 1 It is the schematic diagram of the enzyme-driven bottle-shaped nanomotor swimming in this embodiment;

[0039] figure 2 It is an optical microscope photo of enzyme-driven bottle-shaped nanomotor moving under chemical drive in the present embodiment;

[0040] image 3 It is an optical microscope photo of the enzyme-driven bottle-shaped nanomotor chemotactic movement under the glucose concentration gradient prepared in this example.

[0041] This embodiment provides an enzyme-driven bottle-shaped nanomotor, which includes a bottle-shaped nanoparticle skeleton and two enzymes inside the skeleton. Wherein the bottle-shaped nanoparticles are hydrothermally carbonized carbon-based polymers, and the two enzymes are glucose oxidase and catalase. Moreover, the glucose oxidase and catalase used in this example are commercially available products, wherein the glucose oxidase can decompose glucose, convert the chemica...

Embodiment 2

[0049] See Figure 4 , Figure 5 ,

[0050] Figure 4It is a schematic diagram of step 3 and step 4 in the preparation method of the enzyme-driven bottle-shaped nanomotor in this embodiment;

[0051] Figure 5 It is a transmission electron microscope photo and an energy dispersive X-ray detection photo of the enzyme-driven bottle-shaped nanomotor prepared in this example.

[0052] In this example, using bottle-shaped nanoparticles, glucose oxidase, and catalase as materials, glucose oxidase and catalase are simultaneously loaded inside the bottle-shaped nanoparticles by vacuum infusion and ultrasonic infusion to prepare a Enzyme-driven bottle-shaped nanomotors. Specifically include the following steps:

[0053] Step 1. Preparation of bottle-shaped nanoparticles:

[0054] a. Add 0.0435g polyethylene oxide-polypropylene oxide-polyethylene oxide triblock copolymer (P123) and 0.0365g sodium oleate (SO) into 20mL deionized water to obtain mixed solution A, and Stir at 100rp...

Embodiment 3

[0070] In this example, using bottle-shaped nanoparticles, glucose oxidase, and catalase as materials, glucose oxidase and catalase are simultaneously loaded inside the bottle-shaped nanoparticles by vacuum infusion and ultrasonic infusion to prepare a Enzyme-driven bottle-shaped nanomotors. Specifically include the following steps:

[0071] Step 1. Preparation of bottle-shaped nanoparticles:

[0072] a. Add 0.0435g polyethylene oxide-polypropylene oxide-polyethylene oxide triblock copolymer (P123) and 0.0365g sodium oleate (SO) into 10mL deionized water to obtain mixed solution A, and Stir at a speed of 100rpm / min in a water bath at 25°C for 0.5h;

[0073] b. Dissolve 2g of ribose in 20mL of deionized water, add it to the mixed solution A, and stir in a water bath at a temperature of 25°C for 20min at a speed of 100rpm / min to obtain a mixed solution B;

[0074] c. Transfer the mixed solution B to a 75mL reaction kettle, put it in an oven, keep it at 160°C for 8-20h, and ce...

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Abstract

The invention provides an enzyme-driven bottle-shaped nano-motor and a preparation method thereof. The method includes the steps of: taking bottle-shaped nanoparticles, glucose oxidase and catalase asthe materials, loading glucose oxidase and catalase inside the bottle-shaped nanoparticles simultaneously by vacuum perfusion method and ultrasonic perfusion method so as to prepare the enzyme-drivenbottle-shaped nano-motor. Compared with the prior art, the invention has the beneficial effects: the preparation process is simple, the prepared nano-motor has good biocompatibility and strong controllability, can move along the glucose concentration gradient in a glucose solution, realizes chemotactic movement of the enzyme-driven bottle-shaped nano-motor, and has broad application prospects indrug carrying, toxin removal, tumor therapy and other biomedical fields.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to an enzyme-driven bottle-shaped nanomotor and a preparation method thereof. Background technique [0002] In people's vision of the future, there are often scenes where micro-nano-scale devices or robots enter the human body. People imagine that these micro-nano devices can move in the body and treat some diseases that are difficult to treat by conventional means. In recent years, these imaginations have gradually become reality, and scientists have carried out research on artificial micro-nano motors. The micro-nano motor is a device that uses the energy in the environment to convert chemical energy, light energy, electric energy and other energy into its own kinetic energy. Its driving methods can be divided into two categories. The chemical energy is converted into kinetic energy to drive the motor; the other type is a fuel-free system, which drives the motor thro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/34B81B1/00B81C1/00
CPCA61K47/34B81B1/00B81B2201/03B81C1/00
Inventor 贺强周昶高长永林之华
Owner HARBIN INST OF TECH
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