Reagent for quantitatively detecting ractopamine by latex enhanced immune turbidimetry

A technology of ractopamine and immunoturbidimetry, which is applied in the direction of measuring devices, material analysis by observing the influence on chemical indicators, instruments, etc., to achieve the effects of convenient use, ensuring food safety, and significant economic and social benefits

Inactive Publication Date: 2017-11-24
HENAN LIANBO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] A reagent for the quantitative detection of ractopamine by strong immunoturbidimetric method, which can effectively solve the problem of rapid detection of ractopamine in food and ensure food safety

Method used

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  • Reagent for quantitatively detecting ractopamine by latex enhanced immune turbidimetry
  • Reagent for quantitatively detecting ractopamine by latex enhanced immune turbidimetry
  • Reagent for quantitatively detecting ractopamine by latex enhanced immune turbidimetry

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1: The reagents of the present invention include reagent R1 and reagent R2. Reagent R1 is made of glycine 3.75g, ractopamine-carrier antigen complex 0.2, and distilled water to make up to 1000ml. The carrier protein is bovine serum albumin (BSA). Among them, the preparation method of the ractopamine-carrier antigen complex is: add 150 mg of ractopamine to 4 ml of pyridine solution containing 22.8 mg of glutaric anhydride, react for 22 hours under stirring at room temperature, and the stirring speed is 150 rpm / min, and vacuum dry the reactant after the reaction. Pyridine, to get a precipitate, the precipitate was dissolved in 8ml of DMF and 1,4-dioxane in a 1:1 solvent, added n-tributylamine 52.4μl and isobutyl chloroformate 28.8μl, ice bath reaction 1h , and then add the reaction solution dropwise to 10ml 4°C pre-cooled BSA solution with a mass concentration of 0.2%, react at 4°C for 48 hours, and dialyze the reactant in 0.01M PH=7.2 PBS for 48 hours, changing ev...

Embodiment 2

[0017] Example 2: Reagent R1: glycine 7.505g, ractopamine-carrier protein complex 0.4g,

[0018]Adjust the pH to 7.4 and distill the volume to 1000ml;

[0019] Reagent R2: glycine 7.505g, polyethylene glycol 6000 20g, anti-ractopamine monoclonal antibody cross-linked latex 300mg, bovine serum albumin (BSA) 20g, Proclin300 2ml

[0020] Adjust the pH to 7.4 and distill the volume to 1000ml;

[0021] The preparation method is the same as in Example 1.

Embodiment 3

[0022] Example 3: Reagent R1: Glycine 15.01g, Ractopamine-Carrier Protein Complex 1.1g

[0023] Adjust the pH to 7.4 and distill the volume to 1000ml;

[0024] Reagent R2: glycine 15.01g, polyethylene glycol 6000 30g, anti-ractopamine monoclonal antibody cross-linked latex 600mg, bovine serum albumin (BSA) 30g, Proclin300 5ml

[0025] Adjust the pH to 7.4 and distill the volume to 1000ml;

[0026] The preparation method is the same as in Example 1.

[0027] The use of the present invention is: using a semi-automatic biochemical analyzer or a fully automatic biochemical analyzer; the basic parameters are as follows: reaction temperature 37°C; cuvette optical path 1.0cm; main wavelength 570nm, secondary wavelength 800nm; reaction time 8 minutes; to be tested The amount of items and standard products is 10ul; the items to be tested are feed, pig muscle and its organs, and pig urine, and the standard product is pure ractopamine with a purity of 99.5%.

[0028] Firstly, use seri...

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Abstract

The invention aims to provide a reagent for quantitatively detecting ractopamine by a latex enhanced immune turbidimetry. The reagent comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared by adding distilled water into 3.75-15.01 g of glycine and 0.2-1.1 g of a ractopamine-carrier antigen complex and fixing a volume to 1000 ml; the reagent R2 is prepared by adding distilled water into 1.4-7.0 g of glycine, 10-30 g of polyethylene glycol 6000, 100-600 mg of carboxyl latex and anti-ractopamine monoclonal antibody cross-linked latex, 10-30 g of bovine serum albumin (BSA) and 1-5 m of Proclin 300 and fixing a volume to 1000 ml. The reagent provided by the invention is scientific and reasonable in formula, easy to produce and prepare, and convenient, safe and accurate to use, can be effectively used for quickly detecting the ractopamine in food, ensures the food safety and has significant economic and social benefits.

Description

technical field [0001] The invention relates to a reagent for quantitatively detecting ractopamine by latex-enhanced immune turbidimetry, which belongs to the technical field of food safety detection. Background technique [0002] Ractopamine (RAC) is a synthetic β-adrenoreceptor agonist (commonly known as β-stimulant) compound, molecular weight: 337.83; it was first developed by an American pharmaceutical company with low toxicity and fast metabolism. Clenbuterol substitute, belonging to the second generation of clenbuterol. Because of its ability to regulate protein synthesis, it is also called protein repartitioner (Repartitioner) abroad, and it is mainly used clinically to treat bronchial asthma, congestive heart failure and muscular dystrophy. Ractopamine is far less toxic than other clenbuterol supplements that perform the same function. Conventional doses of clenbuterol drugs can be metabolized and excreted in the body without causing harm to the body. However, exce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/544G01N33/577G01N21/82
CPCG01N33/9413G01N21/82G01N33/54346G01N33/544G01N33/577
Inventor 刘昊旻郑蓬举王威风张倩茹
Owner HENAN LIANBO BIOTECH CO LTD
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