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Preparation method of animal skin acellular matrix dressing and dressing obtained

An acellular matrix and decellularization technology, which is applied in the preparation of animal skin acellular matrix dressings, and in the field of acellular matrix dressings, can solve the adverse effects on the structure and performance of collagen fibers in the acellular dermal matrix, and the removal of DNA/RNA has not attracted enough attention , easy to produce immune rejection and other problems, to achieve the effect of allowing cell repopulation, reducing the risk of transmission, and completely removing antigens

Inactive Publication Date: 2017-11-28
BEIJING NATON TECH GRP CO LTD
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The existing acellular dermal matrix dressings mainly have the following problems: the removal of antigens is not complete, and immune rejection is easy to occur after transplantation; the removal of DNA / RNA has not attracted enough attention; excessive treatment, excessive use of biological reagents and organic reagents Cellular Dermal Matrix Adversely Affects Collagen Fiber Structure and Properties
The study found that freezing and thawing technology can only break the cells, but cannot effectively remove

Method used

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  • Preparation method of animal skin acellular matrix dressing and dressing obtained
  • Preparation method of animal skin acellular matrix dressing and dressing obtained
  • Preparation method of animal skin acellular matrix dressing and dressing obtained

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preparation example Construction

[0033] In one embodiment of the preparation method according to the present invention, the animal skin is preferably common mammalian skin, such as human cadaver skin, pig skin, cowhide, etc., more preferably low-cost pig skin.

[0034] In one embodiment of the preparation method according to the present invention, the decellularization solution comprises trypsin with a mass concentration of 0.01% to 0.09%, ammonium sulfate with a mass concentration of 0.5% to 1.0%, and ammonium sulfate with a mass concentration of 0.5% to 0.15% % degreaser.

[0035] In one embodiment of the preparation method according to the present invention, the degreasing agent can be any type used in the field, including but not limited to Pingpinga, Triton X-100, sodium laurylalanine, fatty alcohol poly Oxyethylene ether etc.

[0036] In one embodiment of the preparation method according to the present invention, the split skin piece is soaked in the decellularization solution for 10-60 minutes, which ...

Embodiment 1

[0090] A cadaver skin slice with a thickness of 0.6 mm was taken with a skin extractor, the external fat was scraped off bluntly, and washed 5 times in purified water for 10 minutes each time. After being washed with purified water, it was placed in a -80°C ultra-low temperature refrigerator for deep freezing for 5 hours. Take out the skin slices from the ultra-low temperature refrigerator, put them into a container filled with purified water at room temperature and soak them until they are completely thawed, and a freeze-thaw process is completed at this time. The thawed skin slices were then frozen in a -80°C refrigerator for 5 hours, and so repeated freezing and thawing 4 times. Then add 5% acetic acid solution and soak for 20 minutes, take it out and then wash it thoroughly with purified water, scrape off the epidermis bluntly, and retain the basement membrane. Put the above cadaver skin slices into a combined solution of 0.06% peracetic acid and 0.5% sodium chloride for ...

Embodiment 2

[0092] Healthy pigs were selected, and after slaughter, a skin slice with a thickness of 0.3 mm was taken with a skinning machine, the external fat was bluntly scraped off, and washed three times in purified water, each time for 15 minutes. After being washed with purified water, it was placed in a -80°C ultra-low temperature refrigerator for deep freezing for 15 hours. Take out the skin slices from the ultra-low temperature refrigerator, put them into a container filled with purified water at room temperature and soak them until they are completely thawed, and a freeze-thaw process is completed at this time. The thawed skin slices were then frozen in a -80°C refrigerator for 15 hours, and so repeated freezing and thawing 3 times. Then add 2% acetic acid solution and soak for 30 minutes, take it out and then wash it thoroughly with purified water, scrape off the epidermis bluntly, and retain the basement membrane. Put the above pigskin into a combined solution of 0.05% perace...

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Abstract

The invention provides a preparation method of animal skin acellular matrix dressing. The preparation method comprises the following steps: a step of inactivation of virus, a degreasing step, an unhairing step and a decellularizing step on split-thickness skin of animal skin, wherein the decellularizing step comprises multiple freeze thawing steps and a decellularizing step by a decellularizing solution after the multiple freeze thawing steps. The invention also provides the animal skin acellular matrix dressing prepared by the preparation method. By means of a decellularizing step combining a physical method and a chemical method, netty structures of elastic fibers and collagens of an extracellular matrix are retained to the maximum extent, and meanwhile, antigens are thoroughly removed. The acellular dermal matrix dressing obtained by the preparation method is permeable and ventilating, can provide a moist micro-environment for the wound, remarkably reduces the rejection reaction of heterogeneity, allows reproliferation of cells, shortens the healing time, reduces cicatricial formation, is convenient to apply, and can be applied to the fields of burn and scale treatment, chronic wound healing, diabetic foot treatment and the like.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a preparation method of an animal skin acellular matrix dressing and the obtained acellular matrix dressing. Background technique [0002] The healing and wound repair of chronic wounds such as burns, wounds, bedsores, diabetic feet, and lower extremity ulcers has always been a major clinical problem. At present, the high-end wet dressings commonly used in the market include hydrocolloid and alginate filling dressings. The disadvantage of hydrocolloid dressings is that they are not suitable for wounds with a lot of exudate and wounds with dead space or depth; the disadvantage of alginate filling dressings is that they are not suitable for dry scab wounds. [0003] A good wound covering can create a microenvironment suitable for tissue repair. Therefore, researchers have been seeking a better wound dressing in recent years. In 1985, Heck et al. reported in The Journal of Tra...

Claims

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Application Information

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IPC IPC(8): A61L15/40A61L15/42
CPCA61L15/40A61L15/42
Inventor 张国强柳轶男杨新广董骧
Owner BEIJING NATON TECH GRP CO LTD
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