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A preparation method of polypeptide hydrogel for encapsulating medicine

A technology for hydrogels and drugs, applied in the field of preparation of polypeptide hydrogels, can solve the problems of long gelation time, several hours or even two or three days, poor water solubility of sequence polypeptides, etc., and achieves simple operation method and good repeatability. Effect

Active Publication Date: 2019-07-09
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although polypeptide hydrogels have many advantages, there are also some sequences of polypeptides with poor water solubility, and the formed hydrogels are not completely transparent. Some sequence polypeptides have good water solubility, but the gelation time is too long, which generally takes more than ten hours. even two or three days

Method used

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  • A preparation method of polypeptide hydrogel for encapsulating medicine
  • A preparation method of polypeptide hydrogel for encapsulating medicine
  • A preparation method of polypeptide hydrogel for encapsulating medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The method of the present invention adopts the conventional solid-phase Fmoc method, that is, the monomer amino acid protected by Fmoc on the solid-phase resin is deprotected to expose the amino group, and forms a peptide bond with the carboxyl group of the amino acid in the solution through a condensation reaction, thereby linking the amino acid to the On the resin, the peptide chain is extended from the C-terminus to the N-terminus.

[0032] 1. Basic materials:

[0033] (1) Resin and linking molecule: The resin selected by the solid-phase Fmoc method is Rink Amide- resin. This kind of resin has very good swelling property, which can make the condensation reaction between the peptide chains better, and there is enough network space to meet the growing peptide chains. HBTU and HOBt are used as linking molecules to immobilize the polypeptide molecules on the resin.

[0034] (2) Monomer: The monomer used in the synthesis is a chemically modified α-amino acid.

[0035...

Embodiment 2

[0052]For the polypeptide hydrogel obtained by the method in Example 1, absorb 500 microliters of PBS buffer solution with a pH of 7.4 and a salt ion concentration of 0.01M on the upper layer of the hydrogel, then place it in a water bath at 37°C, and take out the release solution after 0.5h , and add 500 microliters of new PBS buffer under the same conditions, and replace the buffer again after 1 hour, and the replacement time doubles. Then, the absorbance of the release liquid was measured by an ultraviolet spectrophotometer, and the amount of doxorubicin hydrochloride was calculated according to Lambert-Beer's law, and the calculated contents of multiple release liquids were added to obtain the total release amount. And draw the total release amount-time graph according to the relationship between the total release amount and time. In addition, the PBS buffer conditions were selected as pH 5.5 0.01M and pH 6.5 0.01M, and the hydrochloric acid in the IKFQFHFD polypeptide hydr...

Embodiment 3

[0054] Select the PBS buffer conditions as: pH7.4 0.01M, pH7.4 0.025M and pH7.4 0.05M, and measure the relationship between the amount of doxorubicin hydrochloride released in the IKFQFHFD polypeptide hydrogel and time at 37 ° C, the steps With embodiment 2.

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Abstract

The invention belongs to the field of biological materials, and particularly relates to a preparation method of polypeptide hydrogel for wrapping a medicine. Dissolution is assisted by an ultrasonic concussion method, amphiphilic polypeptide hydrogel is dissolved in deionized water solution of 1mg / ml doxorubicin hydrochloride to obtain doxorubicin hydrochloride water solution with the polypeptide concentration of 10mg / ml, and the polypeptide hydrogel for wrapping doxorubicin hydrochloride is formed by self-assembly. Results indicate that the pH (potential of hydrogen) value of the solution, the concentration of salty ions and environmental temperature have a large effect on release of the medicine in the polypeptide hydrogel. The prepared hydrogel has good biocompatibility and degradability, and has a wide application prospect in medicine release control.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a preparation method of a polypeptide hydrogel which can be used to wrap medicine. Background technique [0002] Hydrogel is a kind of polymer with hydrophilic groups, which can be swelled by water but insoluble in water, and has a three-dimensional network structure. Capacitance and degradability have received more and more attention and research. Polypeptide hydrogel can absorb a large amount of water and swell significantly in water, and maintain its original structure without being dissolved after significant swelling. It can produce corresponding changes to external stimuli, such as: temperature, pH, salt ion concentration and light. Changes in various conditions will affect the volume and stability of the polypeptide hydrogel to varying degrees. This characteristic of polypeptide hydrogel makes it have great potential applications in drug controlled release...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K47/42A61K31/704C07K7/06C07K1/06C07K1/04
CPCY02P20/55
Inventor 王建浩程永江邱琳蒋鹏举柳丽丁淑敏朱志兰顾婷
Owner CHANGZHOU UNIV