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Inactivated vaccine of porcine epidemic diarrhea virus and preparation method of inactivated vaccine

A technology for porcine epidemic diarrhea and virus inactivation, which is used in vaccines, antiviral agents, veterinary vaccines, etc., to achieve the effects of improving the level, simple preparation method, and shortening the immune window period

Active Publication Date: 2017-12-01
广州渔跃生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Schizophyllum polysaccharide (SPG) is a water-soluble polysaccharide extracted from the fruiting body, mycelium and fermentation broth of Schizophyllum fungus. Its structure is β-(1-3) glycoside as the main chain, with β-(1-6 ) β-D-glucan on the glycosidic side chain; canna flower is the flower of canna, a perennial herbaceous plant in the family Cannaceae; indomethacin is an anti-inflammatory drug for nonzizia. enhanced reporting

Method used

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  • Inactivated vaccine of porcine epidemic diarrhea virus and preparation method of inactivated vaccine
  • Inactivated vaccine of porcine epidemic diarrhea virus and preparation method of inactivated vaccine
  • Inactivated vaccine of porcine epidemic diarrhea virus and preparation method of inactivated vaccine

Examples

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Embodiment 1

[0027] Embodiment 1, the preparation of fresh canna flower extract

[0028] Take canna flowers or flower buds, take their petals, grind them, add distilled water, put them in a triangular flask, seal them, take a constant temperature water bath at 95°C for 30 minutes, cool them down rapidly, centrifuge them at room temperature for 10 minutes, and take the supernatant to obtain the final product.

[0029] Embodiment 2-4 of the present invention formula and dosage of immunopotentiator

[0030]

Embodiment 5

[0031] Embodiment 5, the preparation of porcine epidemic diarrhea virus inactivated vaccine of the present invention

[0032] A) Expansion culture of ST cells: take ST cells and digest and disperse them with trypsin, add cell growth medium at 37°C, containing 5% CO 2 Cultured in an environment until the cells form a good monolayer, and then wash the cells with serum-free DMEM medium for inoculation of porcine epidemic diarrhea virus;

[0033] B) Virus inoculation: inoculate the porcine epidemic diarrhea virus seed into the cells prepared in the step according to the final volume of 50:500, absorb the virus solution at 37°C for 45min, and then use the cell growth medium at 37°C, containing 5%CO 2 Cultivate in an environment where the cytopathic rate reaches more than 75%, harvest the cell liquid;

[0034] C) Propagation of seedling venom: Take the cell line culture bottle that has formed a good monolayer in step A), discard the cell growth liquid, inoculate the cell liquid ...

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Abstract

The invention belongs to the field of biological products, and in particular relates to an inactivated vaccine for porcine epidemic diarrhea virus which is produced by ST cells and a preparation method of the inactivated vaccine. The method comprises four steps, namely amplification culture of the ST cells, virus inoculation, multiplying of vaccine producing virus liquid, and product preparation. The vaccine, which is produced by the method, is high in immunization level and short in immunological window phase; an immunity period is obviously prolonged; and total secretory antibody (total SIgA) in intestinal mucosa is remarkably improved.

Description

technical field [0001] The invention belongs to the field of biological products, and in particular relates to an inactivated porcine epidemic diarrhea virus vaccine and a preparation method thereof. Background technique [0002] Porcine epidemic diarrhea is an acute contact intestinal infectious disease of pigs caused by porcine epidemic diarrhea virus (PEDV), characterized by vomiting, diarrhea, loss of appetite and dehydration. Pigs are susceptible. The clinical symptoms and pathological changes of this disease are very similar to porcine transmissible gastroenteritis, but the mortality rate of suckling piglets is relatively low, and the transmission speed in pigs is relatively slow. [0003] PEDV is a member of the genus Coronavirus in the family Coronaviridae, and only one serotype has been found so far. PEDV can persist in pig herds, and pigs of all ages are susceptible. The sickness rate of suckling piglet, shelf pig and fattening pig can reach 100%, especially ser...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61K47/42A61P31/14A61K31/715A61K36/88A61K31/405
CPCA61K31/405A61K31/715A61K36/88A61K39/12A61K47/42A61K2039/5252A61K2039/552A61K2300/00
Inventor 张毓金严悌昆
Owner 广州渔跃生物技术有限公司
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