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A polyamino acid-terminated tissue repair material and preparation method thereof

A technology of polyamino acid and repair material, which is used in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problem of not completely removing the toxicity of cross-linking agents, end-capping treatment toxicity, and increasing the number of free carboxyl groups. and anti-degradation properties, reduce residual toxicity, and stabilize the effect of surface treatment

Active Publication Date: 2020-07-17
重庆大清医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have certain defects: (1) simple cleaning cannot completely remove the toxicity problem caused by the crosslinking agent; (2) complex end-capping treatment may bring new toxicity problems, and the cost is also high; (3) The simple amino acid capping treatment only solves the problem of free aldehyde groups, and may increase the number of free carboxyl groups, and the increase of free carboxyl groups makes the problem of calcification after implantation of materials still serious; (4) The current capping technology It cannot make up for the problem of excessive free carboxyl groups on the surface of materials that have been out of balance

Method used

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  • A polyamino acid-terminated tissue repair material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1 Preparation method of a polyamino acid modified tissue repair material

[0024] Proceed as follows:

[0025] (1) Prepare the porcine small intestinal submucosa after deantigenation, soak it in 0.1M glutaraldehyde pH=8.8 phosphate buffer, put it in a shaker at 37°C to make it fully cross-linked for 12 hours, and take it out with deionized Rinse fully with water; then dry the surface moisture with filter paper, put it into liquid nitrogen for 2 hours and freeze it quickly for 2 hours, and vacuum freeze-dry it at -80°C for 8 hours to obtain the cross-linked ECM;

[0026] (2) Soak the obtained cross-linked ECM in 0.5% polylysine solution with a molecular weight of 6000, shake it in a shaker at 37°C at a speed of 120 rpm for 4 hours, take it out and wash it thoroughly with deionized water . Put it into liquid nitrogen and freeze it quickly for 2 hours, then vacuum freeze-dry it at -80°C for 8 hours to obtain the ECM after cross-linking treatment;

[0027] (3) Th...

Embodiment 2

[0028] Example 2 Preparation method of a polyamino acid modified tissue repair material

[0029] Proceed as follows:

[0030] (1) Prepare the bovine dermis matrix after antigen removal, soak it in 1M sodium chloride solution in MDI, put it in a shaker at 37°C to make it fully cross-linked for 10 minutes, take it out and rinse it with deionized water; then use filter paper Wipe off the surface moisture, freeze in the refrigerator for 6 hours, and vacuum freeze-dry at -20°C for 16 hours to obtain the cross-linked ECM;

[0031] (2) Soak the obtained cross-linked ECM in 0.001% polylysine-aspartic acid copolymer solution with a molecular weight of 100,000, and shake it for 4 hours at a speed of 120 rpm in a shaker at 37°C, Remove and rinse thoroughly with deionized water. Put it into liquid nitrogen and freeze it quickly for 2 hours, then vacuum freeze-dry it at -80°C for 16 hours to obtain the cross-linked ECM;

[0032] (3) The material was soaked in 0.01% SGF solution for 2 ho...

Embodiment 3

[0033] Example 3 Preparation method of a polyamino acid modified tissue repair material

[0034] Proceed as follows:

[0035] (1) Prepare the bovine Achilles tendon matrix after antigen removal, soak it in 1M polyethylene glycol glycidyl ether, put it in a shaker at 37°C to make it fully cross-linked for 20 days, take it out and rinse it with deionized water; then use Dry the surface moisture with filter paper, put it into liquid nitrogen to freeze rapidly for 2 hours, and carry out vacuum freeze-drying at -80°C for 8 hours to obtain the ECM after cross-linking treatment;

[0036] (2) Soak the obtained cross-linked ECM in 40% polylysine solution with a molecular weight of 1000, and shake it in a shaker at 37°C at a speed of 120 rpm for 48 hours, take it out and wash it thoroughly with deionized water . Put it into liquid nitrogen and freeze it quickly for 2 hours, then vacuum freeze-dry it at -80°C for 8 hours to obtain the ECM after cross-linking treatment;

[0037] (3) So...

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Abstract

The invention relates to a preparation method of a tissue repair material modified by polyamino acid. The preparation method is characterized in that an acellular outer matrix material subjected to de-antigen treatment serves as a skeleton structure, after being treated by a cross-linking agent and an end-capping agent, the skeleton structure is compounded with bioactive substance. After treatment of the end-capping agent, the residual toxicity of the cross-linking agent is reduced, the cross-linking groups such as a free aldehyde group with the end capped by a covalent bond are formed, the formed protection is stable, the safety of the product and the anti-calcification capability of the material are improved, meanwhile, polycations are adopted for end capping, so that polyanions (sodium hyaluronate, chondroitin sulfate and the like) can be well adsorbed to the surface of the material through hydrogen bonds, and the surface treatment of the material is more stable; finally, polysaccharide molecules or growth factors with biological activity are modified on the surface of the obtained material, so that the tissue regeneration is promoted, and finally, the tissue repair material with certain strength and anti-calcification capacity is obtained.

Description

technical field [0001] The invention relates to a polyamino acid-terminated tissue repair material and a preparation method thereof, belonging to the technical field of biomedical materials. Background technique [0002] Biomedical materials are materials used to combine with biological systems, treat or replace tissues and organs in biological organisms or enhance their functions. Acellular extracellular matrix material is a relatively new type of tissue repair material, which is mainly composed of fibrous collagen and also contains aminodextran and glycoprotein. Increasingly used in tissue repair surgery. With the application of acellular extracellular matrix materials, problems such as rapid degradation and reduced mechanical strength during tissue regression are gradually exposed, and the cross-linked materials significantly enhance the structural strength and implantation life of biological tissues. [0003] At present, the cross-linking means of this material mainly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/20A61L27/22A61L27/54
CPCA61L27/18A61L27/20A61L27/227A61L27/3633A61L27/54A61L2300/232A61L2300/412A61L2300/414A61L2400/02A61L2400/18C08L77/04C08L5/08C08L5/04C08L89/00
Inventor 牛睿张玉兰李湘杰李次会
Owner 重庆大清医疗器械有限公司
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