Sticky protein hydrogel and preparation method thereof

A hydrogel and protein technology, applied in the field of viscous and tough protein hydrogel and its preparation, can solve problems such as toughness and strength, and achieve good biocompatibility and non-toxic effects

Inactive Publication Date: 2017-12-08
CHANGCHUN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This hydrogel is viscous, but

Method used

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  • Sticky protein hydrogel and preparation method thereof
  • Sticky protein hydrogel and preparation method thereof
  • Sticky protein hydrogel and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0023] Embodiment 1: The steps and conditions of a method for preparing a viscous and tough protein hydrogel are as follows:

[0024] (1) Dissolution of casein

[0025] Dissolve 9g of casein in 60mL of deionized water to prepare a casein solution with a mass concentration of 1.5g / mL. At this time, the casein is insoluble in water. Use a 10M sodium hydroxide solution to adjust the pH to 6.6 and use a disperser to fully Stir for 30 minutes until completely dissolved, then put it in an ultrasonic cleaner for 1 hour to remove air bubbles, and obtain an aqueous solution of casein, and put the solution in a refrigerator at 4°C for later use;

[0026] (2) Preparation of hydrogel

[0027] Add 0 mL (corresponding to 0 g of casein weight) aqueous casein solution, 3.5 g of acrylamide, 0.0175 g of N,N-methylenebisacrylamide and 0.02 g of potassium persulfate into 10 mL of deionized water, and stir at room temperature until completely Dissolve, and finally add 0.0156g tetramethylethylene...

Embodiment 2

[0030] Embodiment 2: The steps and conditions of a method for preparing a viscous and tough protein hydrogel are as follows:

[0031] (1) Dissolution of casein

[0032] Dissolve 9g of casein in 60mL of deionized water to prepare a casein solution with a mass concentration of 1.5g / mL. At this time, the casein is insoluble in water. Use a 10M sodium hydroxide solution to adjust the pH to 6.8 and use a disperser to fully Stir for 40 minutes until completely dissolved, then put it into an ultrasonic cleaner for 1.5 hours to remove air bubbles, and obtain an aqueous solution of casein, put the solution in a refrigerator at 4°C and refrigerate it for later use;

[0033] (2) Preparation of hydrogel

[0034] Add 1.67mL (corresponding to 0.5g of casein weight) aqueous casein solution, 3g of acrylamide, 0.015g of N,N-methylenebisacrylamide and 0.02g of potassium persulfate into 8.33mL of deionized water, and stir at room temperature Until it is completely dissolved, finally add 0.0156...

Embodiment 3

[0036]Embodiment 3: The steps and conditions of a method for preparing a viscous and tough protein hydrogel are as follows:

[0037] (1) Dissolution of casein

[0038] Dissolve 9g of casein in 60mL of deionized water to prepare a casein solution with a mass concentration of 1.5g / mL. At this time, the casein is insoluble in water. Adjust the pH to 7.0 with a 10M sodium hydroxide solution and use a disperser to fully Stir for 50 minutes until completely dissolved, then put it into an ultrasonic cleaner for 1.5 hours to remove air bubbles, and obtain an aqueous solution of casein, put the solution in a refrigerator at 4°C and refrigerate for later use;

[0039] (2) Preparation of hydrogel

[0040] Add 3.33mL (corresponding to 1g of casein) aqueous solution of casein, 2.5g of acrylamide, 0.0125g of N,N-methylenebisacrylamide and 0.02g of potassium persulfate into 6.67mL of deionized water, and stir at room temperature Until it is completely dissolved, finally add 0.0156g of tetr...

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Abstract

The invention discloses sticky protein hydrogel and a preparation method thereof and belongs to the technical field of high polymer hydrogel. According to the method, casein protein (casein) in a milk protein serves as a raw material. The preparation method comprises the following steps: firstly, regulating an aqueous solution of casein protein by a sodium hydroxide solution, and fully dispersing in a dispersion device to enable the casein protein to be completely dissolved in water; then adding the casein protein solution, a hydrophilic monomer acrylamide, a chemical cross-linking agent N,N-methylenebisacrylamide and an initiator potassium persulfate into deionized water, stirring at a room temperature to be completely dissolved; adding an aid tetramethylethylenediamine to be uniformly mixed, and directly pouring the prepared solution into a mold; finally, putting the mold into a drying oven to carry out a redox reaction, and enabling the casein protein and the hydrophilic monomer to perform free radical polymerization, thereby obtaining the high polymer hydrogel. The casein protein is the main protein in milk of mammals including cows, sheep and humans, has excellent biocompatibility, does not have any toxicity and is readily available in raw material and low in cost. The hydrogel has toughness and stickiness and can be applied to the fields of drug controlled release, drug dressings and the like.

Description

technical field [0001] The invention belongs to the technical field of macromolecule hydrogel, and in particular relates to a viscous and tough protein hydrogel and a preparation method thereof. Background technique [0002] Hydrogel is a kind of water-soluble polymer material with water as the dispersion medium and a network cross-linked structure, which contains a part of hydrophobic groups and hydrophilic groups, wherein the hydrophilic groups combine with water molecules to connect water molecules network, while the hydrophobic groups can swell with water to form cross-linked polymers. Hydrogels have good biocompatibility and can be used in biomedical fields and drug delivery systems. [0003] Chinese patent (CN 105111341A) describes a viscous nano-hybrid hydrogel with high mechanical strength. , polyvinyl alcohol and cyclodextrin aqueous dispersion; the preparation method of the viscous hydrogel includes dispersing the inorganic nano-crosslinking agent lethionite and ...

Claims

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Application Information

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IPC IPC(8): C08F289/00C08F220/56C08F222/38
CPCC08F289/00C08F220/56
Inventor 高光辉许健宇任秀艳
Owner CHANGCHUN UNIV OF TECH
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