A preparation method of photodynamic therapy agent guided by t1-mri imaging

A T1-MRI, imaging-guided technology, applied in the field of photodynamic therapeutic agent preparation under the guidance of T1-MRI imaging, can solve the problems of strong oxygen dependence, difficult monitoring, restricted development, etc. The effect of reducing toxic side effects

Active Publication Date: 2020-06-26
HUBEI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, the main problems of photodynamic therapy are strong oxygen dependence, low dose of sensitizer reaching the tumor site, and difficult monitoring before and after treatment, which limit its development.

Method used

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  • A preparation method of photodynamic therapy agent guided by t1-mri imaging
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  • A preparation method of photodynamic therapy agent guided by t1-mri imaging

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preparation example Construction

[0042] In an embodiment of the present invention: a method for preparing a photodynamic therapeutic agent guided by T1-MRI imaging, comprising the following steps:

[0043] The first step: preparing nanoparticles of gadolinium (manganese)-porphyrin metal organic framework structure; the specific preparation method is as follows:

[0044] (1) Preparation of trivalent gadolinium ion solution (divalent manganese ion solution), its trivalent gadolinium salt is a kind of in gadolinium chloride hexahydrate, gadolinium sulfate or nonahydrate gadolinium nitrate; its divalent manganese salt is chlorine tetrahydrate One of manganese chloride, manganese nitrate tetrahydrate, manganese nitrate tetrahydrate manganese carbonate; specifically weigh 3.72mg GdCl 3 ·6H 2 O is dissolved in the organic solvent (1mmol / L) of 10mL, standby; Then weigh 1.98mg MnCl 2 4H 2 O is dissolved in 10mL of organic solvent (1mmol / L), and the organic solvent is one of N,N-dimethylformamide (DMF), N,N-dimethylac...

Embodiment 1

[0067] Cytotoxicity test: After the prepared nanocomposite material was co-cultured with 4T1 cells for 24 hours, the relative survival rate of the cells was detected by the MTT method. It was found that the composite material had good biocompatibility. At the maximum concentration of the experiment, the cells The relative survival rate can still reach more than 80%.

Embodiment 2

[0069] In vitro photodynamic therapy: After the prepared nanocomposite was co-cultured with 4T1 cells for 24 hours, a 660nm laser was used at 60mW / cm 2 After irradiating at high power for 10 minutes and culturing in a cell incubator for 8 hours, the relative survival rate of the cells was detected by the MTT method. The results showed that the prepared nanocomposite material had a good photodynamic therapy effect on tumor cells.

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Abstract

The invention discloses a preparation method of a T1-MRI imaging guided photo-active therapeutic agent. The method comprises the following steps: preparing nano-particles having a gadolinium (manganese)-porphyrin metal organic frame structure; preparing a protein / sulfadiazine compound; mixing the nanoparticles with the compound to prepare a bovine serum albumin / sulfadiazine-gadolinium (manganese) porphyrin composite nanomaterial, and using the photo-active effect of the composite nanomaterial under the guidance of T1-MRI to prepare the photo-active therapeutic agent. The T1-MRI imaging guided photo-active therapeutic agent is a composite material having a protein / sulfadiazine-gadolinium (manganese) porphyrin metal organic frame structure, the prepared composite material has very good biocompatibility and low cytotoxicity, and a result of MTT test proves that the composite material can trigger the photo-active effect under the illumination of 660 nm laser, and can kill the tumor cells. The prepared composite material is a very good T1-MRI contrast agent, can be used for the screening and guided photo-active therapy of tumors, and has great potential application values in the field of treatment of tumors.

Description

technical field [0001] The invention relates to the technical fields of material science and biomedicine, in particular to a method for preparing a photodynamic therapeutic agent guided by T1-MRI imaging. Background technique [0002] At present, malignant tumors, also known as cancer, have become one of the diseases that threaten human health the most. The earlier the tumor is found, the better the treatment of the tumor will be. Magnetic resonance imaging (MRI) is currently one of the most powerful detection methods for clinical diagnosis of tumors. It has the advantages of high resolution, no ionizing radiation damage, and multi-parameter, multi-sequence imaging. In order to achieve clearer and more accurate imaging, contrast agents are usually used clinically to improve the contrast of the imaging site and obtain more comprehensive information about the lesion. Therefore, it is particularly important to develop novel MRI contrast agents. Substances with paramagnetism ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/62A61K41/00A61K49/08A61K49/18A61K47/64A61K47/69A61K47/54A61P35/00B82Y30/00B82Y5/00
CPCA61K41/0076A61K49/08A61K49/1827A61K49/1833A61K49/1869B82Y5/00B82Y30/00
Inventor 徐祖顺朱伟李全涛王晶阳哲张力
Owner HUBEI UNIV
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