A method for detecting bisphenol A based on the principle of acetylcholinesterase signal amplification
A technology of acetylcholinesterase and signal amplification, which is applied in the field of detection, can solve the problems of in-situ detection, etc., and achieve the effect of simple and portable equipment, good selectivity, and low detection limit
Active Publication Date: 2019-05-24
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF3 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] Aiming at the deficiencies in this field, the present invention aims at the inability to realize in-situ detection by large-scale detection instruments that the existing bisphenol A detection relies on, and provides a method based on the amplification principle of acetylcholinesterase, through general-purpose pH test paper or pH Method for realizing quantitative detection of bisphenol A
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0035] (1) Preparation of magnetic nanoparticles (Fe 3 o 4 );
[0036] Magnetic nanomaterials (Fe 3 o 4 ) were synthesized by hydrothermal method. Accurately weigh 2g of ferric chloride hexahydrate and 4.8g of sodium acetate and dissolve them in 60mL of ethylene glycol, then add 1.5g of polyethylene glycol and fully stir for 2 hours under the action of a mechanical stirrer, and transfer all the yellow liquid to a container with polyethylene glycol In a sealed stainless steel hydrothermal reaction kettle with a tetrafluoroethylene liner (100 mL), heat and react in an oil bath at 210° C. for 12 h. After the reaction was completed, the obtained black substance was repeatedly washed 6 times with absolute ethanol, and then vacuum-dried at room temperature.
[0037] The resulting magnetic nanomaterials (Fe 3 o 4 ) see figure 1 The a, its particle size is about 100nm.
[0038] (2) Preparation of aptamer-functionalized magnetic nanoparticles, integrating the excellent charact...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
Login to View More
Abstract
The invention provides a bisphenol A detection method based on an AchE (acetylcholine esterase) signal amplification principle. 1-(3-dimethylamino propyl)-3-ethylcarbodiimide hydrochloride and NHS (N-hydroxysuccinimide) are added to a Fe3O4 magnetic nanoparticle suspension for activation, the activated suspension is coupled with an aptamer DNA1, and a Fe3O4-DNA1 suspension is prepared; AuNPs are modified with an aptamer complementary chain DNA2 and AchE, and a DNA2-AuNPs-AchE polymer is formed; the Fe3O4-DNA1 suspension, a to-be-detected sample and the DNA2-AuNPs-AchE polymer solution are mixed, and the pH is detected. According to the method, functionalization of AchE and the complementary chain is performed with a gold nanomaterial as a carrier, assembly of functional gold / magnetic nanoparticles is driven by a target product, a linear relation between the concentration of the target product and the pH value is established, and the detection method is convenient, quick, high in sensitivity and high in selectivity.
Description
technical field [0001] The invention belongs to the detection field, and in particular relates to a method for detecting bisphenol A based on the signal amplification principle of acetylcholinesterase. Background technique [0002] In recent years, food safety incidents related to bisphenol A have occurred frequently, which have had a certain impact and harm on people's physical and mental health. Bisphenol A is the main raw material for the production of various polymer materials such as polycarbonate and epoxy resin, and can also be used in the production of fine chemicals such as antioxidants, coatings, and pesticides. The residues of bisphenol A in food mainly come from food raw materials and food packaging materials: (1) Bisphenol A is difficult to degrade in the environment and widely exists in nature, especially in environmental water and plastic products, and is abundant in organisms. (2) Since bisphenol A is fat-soluble, in products such as canned food with high fa...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N21/78
CPCG01N21/78G01N33/54326
Inventor 许宙程云辉唐瑶陈茂龙汪荣梅博鲁士珍
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY