Cadmium-contaminated paddy field soil microbial passivating agent
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A technology of microorganism and cadmium pollution, applied in the field of biochemistry, can solve the problems of increasing the pH of the solution and reducing the bioavailability of Cd, and achieves the effects of excessive adsorption, good popularization and application value, and reduced bioavailability.
Active Publication Date: 2017-12-19
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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During the growth process, Pseudomonas alcaligenes will also use hydrogen protons and release hydroxide ions to increase the pH of
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Embodiment 1
[0024] Culture of Pseudomonas alcaligenes
[0025] Strains: Pseudomonas alcaligenes (DSM-50342 or DSM-19550).
[0026] Medium: Yeast powder 5.0g, KNO 3 1.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 1.0g, FeCl 3 ·6H 2 O0.05g, CaCl 2 2H 2 O 0.2g, trisodium citrate 5.0g, distilled water 1000mL, pH 7.2-7.4.
[0027] Cultivation: Inoculate 2 rings of slant strains into 100ml liquid medium, place at 30°C, shake at 120rpm for 2-3 days, then transfer to fresh medium according to 10% inoculation amount and expand culture for 48 hours, effective The number of bacteria is 1.0-2.0×10 9 cfu / g.
Embodiment 2
[0029] Cultivation of Bacillus subtilis
[0030] Strains: Bacillus subtilis (DSM-100605, or DSM-8565, or DSM-2109)
[0032] Cultivation: Inoculate 2 rings of slant strains into 100ml liquid medium, place at 30°C, shake at 120rpm for 2-3 days, then transfer to fresh medium according to 10% inoculation amount and expand culture for 48 hours, effective The number of bacteria is 3.0-5.0×10 9 cfu / g.
Embodiment 3
[0034] Culture of sulfate-reducing bacteria
[0035] Sulfate-reducing bacteria strains: Desulfurization Enterobacteriaceae (DSM574) or Desulfovibrio Desulfurization (DSM 642), or a mixture thereof;
[0036] Medium: KNO 3 1.0g, K 2 HPO 4 0.5g, NH4Cl 1.0, Na 2 SO 4 1.0, CaCl 2 .2H 2 O 0.1, MgSO 4 ·7H 2 O 2.0g, sodium lactate 2.0g, yeast extract 1.0g, FeSO 4 ·7H 2 O 0.5g, distilled water 1000mL, pH 7.6-7.8.
[0037] Cultivation: inoculate 2 rings of slant strains into 100ml liquid medium, place at 37-40°C, 50rpm shaker and shake for 7 days, then transfer to fresh medium according to 10% inoculation amount and expand culture for 7-9 days , the effective bacterial count is 3.0-5.0×10 8 cfu / g.
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Abstract
The invention relates to the technical field of biochemistry and aims at providing a cadmium-contaminated paddy field soil microbial passivating agent. According to the technical scheme, for the cadmium-contaminated paddy field soil microbial passivating agent and application, the cadmium-contaminated paddy field soil microbial passivating agent is prepared from the following components including pseudomonas alcaligenes, bacillus subtilis, sulfate reducing bacteria, nutrients and a carrier. In microorganisms, the pseudomonas alcaligenes and the bacillus subtilis are mainly solidified by directly or indirectly adsorbing cadmium ions onto the surfaces of the microorganisms or into cells, so that the migration and the bioavailability of cadmium are lowered; and in addition, the pseudomonas alcaligenes has the effect of regulating a pH value of the soil. In the microorganisms, the sulfate reducing bacteria enables cadmium to be solidified in the soil by mainly reducing sulfate into sulfur ions to form cadmium sulfide with the cadmium. By applying the cadmium-contaminated paddy field soil microbial passivating agent, the cadmium in the soil can be passivated, and absorption of the cadmium ions by rice is greatly eliminated. The technical application is low in cost and convenient to operate and has very good popularization and application values.
Description
technical field [0001] The invention relates to the technical field of biochemistry, in particular to a microbial passivator for treating cadmium-contaminated soil and its application in the solidification and passivation of cadmium in paddy field soil. Background technique [0002] Rice is an important food crop in my country. In recent years, cadmium has been widely used in smelting, chemical and electronic industries, which makes soil cadmium (Cd) pollution increasingly serious. How to effectively prevent and solve the problem of Cd pollution in paddy field soil and maintain the health and safety of soil production has attracted more and more attention in our country. [0003] Cd is a heavy metal element with strong biological toxicity. When it enters the human body, it will form bioaccumulation and damage the liver, kidney, brain and bones. The United Nations Environment Program and the International Occupational Health and Heavy Metals Committee have identified it as ...
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