Preparation method of nutritional fertilizer for preventing occurrence of plant leaf diseases
A technology of plant leaves and nutrient fertilizers, applied in the direction of animal carcass fertilizers, nitrogen fertilizers, potassium fertilizers, etc., can solve the problems of slow growth of plant fruits, less fruit, loss, etc., to avoid the production of toxic metabolites, adequate nutrition, and safety good performance
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[0029] Example 1
[0030] A preparation method of nutrient fertilizer for preventing plant leaf diseases, including the following steps:
[0031] 1) Mix pig temples, chicken feathers and human hair according to the mass ratio of 3:1:2, crush and pass through a 200-mesh sieve, take the sieve and obtain the mixed hair crushed product;
[0032] 2) Mix the mixed hair fragments described in step 1) with pH 6.8 PBS buffer to obtain a mixed suspension, add lipase to the mixed suspension, and enzymatically react at 60°C for 60 min. Filtering and taking the filter residue to obtain the degreasing mixed hair crushed material; the added amount of the lipase is 1% of the mass of the mixed hair crushed material;
[0033] 3) Mix the degreasing mixed hair fragments with a dilute hydrochloric acid aqueous solution with a pH of 4.5, acidolyze at 60°C for 40 min and filter, and the resulting filtrate is neutralized with alkali to pH 7.0 to obtain acid hydrolyzed amino acid solution 1; The mass-volume ...
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[0039] Example 2
[0040] A preparation method of nutrient fertilizer for preventing plant leaf diseases, including the following steps:
[0041] 1) Mix pig temples, chicken feathers and human hair according to the mass ratio of 4:2:3, crush and pass through a 200-mesh sieve, take the sieve and obtain the mixed hair shreds;
[0042] 2) Mix the mixed hair fragments described in step 1) with pH 7 PBS buffer to obtain a mixed suspension, add lipase to the mixed suspension, and filter after enzymatic hydrolysis at 70°C for 70 min. Taking the filter residue to obtain the degreasing mixed hair fragment; the added amount of the lipase is 2% of the mass of the mixed hair fragment;
[0043] 3) Mix the degreasing mixed hair fragments with a dilute hydrochloric acid aqueous solution with a pH of 5.0, acidolyze at 65°C for 45 minutes and then filter, and the resulting filtrate is neutralized with alkali to pH 7.0 to obtain acid hydrolyzed amino acid solution 1; The mass-volume ratio of the mixed...
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[0049] Example 3
[0050] A preparation method of nutrient fertilizer for preventing plant leaf diseases, including the following steps:
[0051] 1) Mix pig temples, chicken feathers and human hair according to the mass ratio of 5:3:4, crush and pass through a 200-mesh sieve, take the sieve and obtain the mixed hair crushed product;
[0052] 2) Mix the mixed hair fragments described in step 1) with pH 7.2 PBS buffer to obtain a mixed suspension, add lipase to the mixed suspension, and enzymatically react at 80°C for 80 min. Filtering and taking the filter residue to obtain a degreasing mixed hair crushed material; the added amount of the lipase is 3% of the mass of the mixed hair crushed material;
[0053] 3) Mix the degreasing mixed hair fragments with a dilute hydrochloric acid aqueous solution with a pH of 5.5, acidolyze at 70°C for 50 min and filter, and the resulting filtrate is neutralized with alkali to pH 7.0 to obtain acid hydrolyzed amino acid solution 1; The mass-volume ra...
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