Preparation method of nutritional fertilizer for preventing occurrence of plant leaf diseases
A technology of plant leaves and nutrient fertilizers, applied in the direction of animal carcass fertilizers, nitrogen fertilizers, potassium fertilizers, etc., can solve the problems of slow growth of plant fruits, less fruit, loss, etc., to avoid the production of toxic metabolites, adequate nutrition, and safety good performance
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Embodiment 1
[0030] A preparation method of a nutrient fertilizer for preventing plant leaf diseases from occurring, comprising the steps of:
[0031] 1) Mix pig sideburns, chicken feathers and human hair according to the mass ratio of 3:1:2, crush them and pass through a 200-mesh sieve, and take the under-sieve to obtain the mixed hair fragments;
[0032] 2) Mix the broken hair mixture described in step 1) with PBS buffer at pH 6.8 to obtain a mixed suspension, add lipase to the mixed suspension, and perform enzymatic hydrolysis reaction at 60°C for 60 minutes Filtrate and take the filter residue to obtain degreasing mixed hair fragments; the added amount of the lipase is 1% of the mass of the mixed hair fragments;
[0033] 3) Mix the degreased mixed hair fragments with dilute hydrochloric acid aqueous solution with a pH value of 4.5, acid hydrolyze at 60°C for 40 min, and then filter, and the obtained filtrate is neutralized with alkali to pH 7.0 to obtain acid hydrolyzed amino acid solu...
Embodiment 2
[0040] A preparation method of a nutrient fertilizer for preventing plant leaf diseases from occurring, comprising the steps of:
[0041] 1) Mix pig sideburns, chicken feathers and human hair according to the mass ratio of 4:2:3, crush them and pass through a 200-mesh sieve, and take the under-sieve to obtain the mixed hair fragments;
[0042] 2) Mix the broken hair mixture described in step 1) with PBS buffer at pH 7 to obtain a mixed suspension, add lipase to the mixed suspension, perform enzymatic hydrolysis reaction at 70°C for 70 minutes, and then filter. Take the filter residue to obtain degreasing mixed hair fragments; the added amount of the lipase is 2% of the mixed hair fragments;
[0043] 3) Mix the degreased mixed hair fragments with dilute hydrochloric acid aqueous solution with a pH value of 5.0, acid hydrolyze at 65°C for 45 minutes and then filter, and the obtained filtrate is neutralized with alkali to pH 7.0 to obtain acid hydrolyzed amino acid solution 1; de...
Embodiment 3
[0050] A preparation method of a nutrient fertilizer for preventing plant leaf diseases from occurring, comprising the steps of:
[0051] 1) Mix pig sideburns, chicken feathers, and human hair according to the mass ratio of 5:3:4, crush them, pass through a 200-mesh sieve, and take the sieved material to obtain the mixed hair fragments;
[0052] 2) Mix the broken hair mixture described in step 1) with PBS buffer at pH 7.2 to obtain a mixed suspension, add lipase to the mixed suspension, and perform enzymatic hydrolysis reaction at 80°C for 80 minutes Filtrate, take the filter residue, and obtain the degreasing mixed hair broken material; the addition amount of the lipase is 3% of the mixed hair broken material;
[0053] 3) Mix the degreased mixed hair fragments with dilute hydrochloric acid aqueous solution with a pH value of 5.5, acid hydrolyze at 70°C for 50 minutes, and then filter, and the obtained filtrate is neutralized with alkali to pH 7.0 to obtain acid hydrolyzed ami...
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