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Charge reversible ion exchange resins, chromatography column, method, and system thereof

A technology of ion-exchange chromatography and ion-exchange groups, which is applied in the direction of ion exchange, anion exchange, and cation exchange materials, and can solve problems such as difficult removal

Active Publication Date: 2017-12-29
DIONEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such polymeric ions tend to form very strong complexes with the stationary phase which, once formed, can be extremely difficult to remove

Method used

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  • Charge reversible ion exchange resins, chromatography column, method, and system thereof
  • Charge reversible ion exchange resins, chromatography column, method, and system thereof
  • Charge reversible ion exchange resins, chromatography column, method, and system thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1-DV

[0103] Example 1 - DVB / EVB particles

[0104] This example describes the preparation of resin carrier particles based on divinylbenzene and ethylvinylbenzene, known as DVB particles. A solution of 20 grams of 75% benzoyl peroxide in a mixture of 230 grams (g) of divinylbenzene (actually 55% divinylbenzene) and 230 grams of ethylvinylbenzene can be added as fine droplets The form was dispersed in 1600 mL of an aqueous solution containing water and 8 grams of polyvinyl alcohol (Polysciences Inc. Cat. No. 4398, 125,000 g / mole, 88% hydrolyzed). The entire mixture can be shielded from air by maintaining a nitrogen atmosphere within the reaction vessel. The mixture can be heated to 80°C and held at this temperature for twenty hours, during which time polymerization occurs. The liquid can be drained from the resin pellets and then can be washed with water to remove water soluble products, resulting in a white opaque polymer in the form of spherical particles.

example 2

[0105] Example 2-Vinylbenzyldimethylglycine

[0106] This example describes the synthesis of hydrolyzed dimethylglycine ethyl ester (DMGEE) quaternized with vinylbenzyl chloride (VBC), a monomer with tunable ionization states and known as vinylbenzyldimethyl Glycine (see Figure 9 ). 9.955 g H 2 O was added to tall vials (~1" diameter x 5" height). Subsequently, a first portion of 1.962 grams of DMGEE was added to the tall vial and then stirred at room temperature until homogeneous. 10.119 grams of VBC was added to the tall vial and the tall vial was then placed on the stirrer set to medium speed. After 4 hours and 44 minutes, a second portion of 2.125 grams of DMGEE was added to the stirred mixture. About 18 hours after the last addition, a third portion of 2.020 grams of DMGEE was added to the stirred mixture. About 9 hours after the last addition, a fourth portion of 2.163 grams of DMGEE was added to the stirred mixture. About 5 days after the last addition, a fifth ...

example 3

[0108] This example describes the grafting of copolymers to DVB particles (see Figure 10 ) and subsequently packed into the column. 4.007 g of DVB granules from Example 1, 0.613 g of sodium styrene sulfonate, 0.326 g of trimethylvinylbenzyl ammonium chloride, 1.008 g of VBC quaternized hydrolyzed dimethylglycine ethyl Ester, 0.347 grams of V50 initiator (a water-soluble cationic azo initiator, which is available from Wako Specialty Chemicals), 0.515 grams of 2M HNO 3 and 5.096 g H 2 O combined in a container and shaken vigorously to homogenize the slurry. Subsequently, the mixture was sonicated for 2 minutes and the container was then placed on a rotating drum in a 62°C oven for 4.75 days. The resin forms in the container as a dry solid mass. Add water to the container and sonicate to remove resin. Additionally, 20 mL of 1M sodium acetate was added to the vessel and sonicated to remove any remaining resin. Transfer the sonication liquid to the sample cup containing the ...

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Abstract

An ion exchange chromatographic packing material is described that includes a copolymer grafted to support resin particles. The copolymer includes an ion exchange group, an ionic crosslinking group configured to ionically bind to the ion exchange group, and an adjustable ionization state group having at least a first net charge at the first pH and a second net charge at the second pH. An overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to the overall second net charge of the chromatographic packing material. This allows impurities to be removed from the chromatographic packing material at the second pH.

Description

technical field [0001] The field of the invention relates to chromatography packing materials or resins in applications such as ion chromatography, liquid chromatography and high pressure liquid chromatography. Background technique [0002] Chromatography is a widely used analytical technique for the chemical analysis and separation of molecules. Chromatography involves the separation of one or more analyte species from other matrix components present in a sample. The stationary phase of a column is usually chosen so that it interacts with the analyte. Such interactions can be ionic, hydrophilic, hydrophobic or combinations thereof. For example, the stationary phase can be derivatized with ionic moieties that will ideally bind to ionic analytes and matrix components with varying levels of affinity. The mobile phase permeates the stationary phase and competes with analytes and matrix components for binding to the ionic moieties. Mobile phase or eluent is the term used to ...

Claims

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Application Information

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IPC IPC(8): C08F212/36C08F212/12C08F257/02C08F212/14B01J20/281B01D15/22
CPCB01D15/22B01J20/281C08F212/12C08F212/36C08F257/02C08F212/14B01D15/361B01J41/14B01J41/20B01J20/3071B01J39/05B01J41/05B01J39/20B01J39/26G01N30/96
Inventor C·A·波尔
Owner DIONEX CORP
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