Method for preparing high-purity alpha-linolenic acid through beta-cyclodextrin embedded coupling-simulation moving bed chromatographic separation

A simulated moving bed and chromatographic separation technology, applied in the field of β-cyclodextrin embedding combined with simulated moving bed chromatographic separation to prepare high-purity α-linolenic acid, can solve the problems of unable to separate and purify fatty acids, and facilitate the realization of industrial production , High preparation efficiency and easy operation

Inactive Publication Date: 2018-01-09
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is impossible to screen specific fatty acids from fatty acids for separation and purification by simply using the β-cyclodextri

Method used

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  • Method for preparing high-purity alpha-linolenic acid through beta-cyclodextrin embedded coupling-simulation moving bed chromatographic separation
  • Method for preparing high-purity alpha-linolenic acid through beta-cyclodextrin embedded coupling-simulation moving bed chromatographic separation
  • Method for preparing high-purity alpha-linolenic acid through beta-cyclodextrin embedded coupling-simulation moving bed chromatographic separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] This example illustrates the process of extracting triglycerides in olives, perilla seeds, linseeds, and silkworm chrysalis.

[0015] Mix the dried above-mentioned powders with the solvent diethyl ether at a mass ratio of 1:50, heat and extract for 0.5 h, remove impurities by suction filtration, and spin evaporate under reduced pressure to obtain crude fat, which is dehydrated and then refined to obtain refined triglycerides. The fatty acid compositions of the triglycerides from the four raw materials are shown in Table 1.

[0016] Table 1 Fatty acid composition of different raw materials (%)

[0017]

Embodiment 2

[0019] This example illustrates the process of pretreating fatty acid ethyl esters with β-cyclodextrin to remove impurities.

[0020] Mix β-cyclodextrin: free fatty acid ethyl ester (V:V) = 1:1, the reaction temperature is 120°C, the rotor speed is 1000rpm, and the reaction is 10h. After cooling at -20°C for 15h, suction filtration is performed. , after drying for 6 hours, an effective amount of petroleum ether was added for decomplexation reaction, and the collected liquid components were rotary evaporated to obtain refined fatty acid ethyl esters. The obtained refined fatty acid ethyl ester is detected by gas chromatography to obtain the free fatty acid composition and content of each oil after pretreatment. After pretreatment with β-cyclodextrin, the unsaturated fatty acid content of silkworm chrysalis oil, perilla seed oil, linseed oil, and olive oil increased by 7.2%, 1.7%, 1.2%, and 5.5%, respectively, and the purity of α-linolenic acid increased respectively. 3%, 1.9%,...

Embodiment 3

[0024] This example illustrates the process of pretreating fatty acid ethyl esters with β-cyclodextrin to remove impurities.

[0025]Mix β-cyclodextrin: free fatty acid ethyl ester (V:V) = 15:1, react at 20°C for 0.5h at a rotor speed of 100rpm, cool at -20°C for 15h and pump After filtering and drying for 6 hours, an effective amount of petroleum ether was added for decomplexation reaction, and the collected liquid components were rotary evaporated to obtain refined fatty acid ethyl esters. The obtained refined fatty acid ethyl ester is detected by gas chromatography to obtain the free fatty acid composition and content of each oil after pretreatment. After β-cyclodextrin pretreatment, the unsaturated fatty acid content of silkworm chrysalis oil, perilla seed oil, linseed oil, and olive oil increased by 2.7%, 3.4%, 1.3%, and 2.7%, respectively, and the purity of α-linolenic acid increased respectively. 1.8%, 0.8%, 0.7%, 0.3%.

[0026] Table 3 Free fatty acid composition of ...

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Abstract

The invention discloses a method for preparing high-purity alpha-linolenic acid through beta-cyclodextrin embedded coupling-simulation moving bed chromatographic separation. The method comprises the following steps: converting raw oil into free fatty acid ethyl esters, removing impurities through a beta-cyclodextrin embedding method, and separating the pretreated fatty acid ethyl esters by utilizing simulated moving bed preparative chromatography, thereby obtaining the alpha-linolenic acid ethyl ester with the purity of more than 95%. The product prepared by the method disclosed by the invention is high in purity, the process is continuous, the operation is simple and convenient, the technical problems that the conventional alpha-linolenic acid ethyl ester separation process is complicatedand discontinuous, and the purity is difficulty controlled are solved, the industrial production of the alpha-linolenic acid ethyl ester can be realized, and the method is a novel pathway for diversified comprehensive utilization of oil resources and high-efficiency separation of the high-purity alpha-linolenic acid.

Description

technical field [0001] The invention belongs to the technical field of food engineering, and in particular relates to a method for preparing high-purity alpha-linolenic acid by embedding beta-cyclodextrin combined with a simulated moving bed chromatography separation method. Background technique [0002] α-linolenic acid, also known as 9,12,15-octadecatrienoic acid (cis 9,12,15-oc-tadecatrienoic acid), is an essential unsaturated fatty acid. Studies have shown that α-linolenic acid and its long-chain metabolites play a key role in many physiological metabolic activities. Such as: it can effectively prevent cardiovascular disease, enhance immunity, lower blood sugar, reduce inflammation, regulate blood lipid metabolism, inhibit the occurrence and spread of cancer, enhance memory, improve vision, and anti-aging effects. Clinical studies have shown that α-linolenic acid can effectively treat some cardiovascular and cerebrovascular diseases, and prevent and treat hyperlipidemia...

Claims

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Application Information

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IPC IPC(8): C07C67/03C07C67/48C07C67/56C07C69/587C11C3/10
Inventor 王俊刘曦潘希伟朱长通盛晟吴福安
Owner JIANGSU UNIV OF SCI & TECH
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