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Bifidobacterium bifidum M2017063 and application thereof in preparation of drugs for relieving symptoms of chronic renal diseases

A technology of Bifidobacterium bifidum and strains, applied in the field of microbiology, can solve the problems of unsatisfactory effect and high cost of kidney transplantation, and achieve the effect of strong ability to remove creatinine and urea nitrogen, high research value and application value

Inactive Publication Date: 2018-01-16
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods require a lot of economic foundation, and the effect is not ideal. Kidney transplantation is not only expensive, but the survival period after transplantation is generally 5-7 years

Method used

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  • Bifidobacterium bifidum M2017063 and application thereof in preparation of drugs for relieving symptoms of chronic renal diseases
  • Bifidobacterium bifidum M2017063 and application thereof in preparation of drugs for relieving symptoms of chronic renal diseases
  • Bifidobacterium bifidum M2017063 and application thereof in preparation of drugs for relieving symptoms of chronic renal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation and identification of Bifidobacterium bifidum CCTCC M2017063

[0023] Bifidobacterium bifidum CCTCC M2017063 was isolated from feces of healthy young children. Dissolve 1g of feces in 10mL of 10% peptone solution, stir and dissolve the glass rod fully, filter out the residue with filter paper, collect the filtrate and centrifuge at 5000rmp for 10min, discard the supernatant, and resuspend the precipitate with 1mL of 10% peptone water. Inoculate 1% into the liquid medium of MRS+0.5% L-cysteine, and culture overnight at 37°C anaerobically. The culture solution was serially diluted 10 times, and 10 -4 、10 -5 、10 -6 、10 -7 1 mL of the gradient dilution solution was evenly spread on the agar plate of MRS+0.5% L-cysteine, and cultured anaerobically at 37°C for 48h. Pick a single colony, separate it by streaking on the plate, carry out repeated purification and culture, observe the colony morphology with Gram staining method to select Gram-positive ba...

Embodiment 2

[0026] Embodiment 2: the effect of bacterial strain on adenine-induced chronic kidney injury mice

[0027] 30 female Balb / C mice were randomly divided into three groups, normal group (10): intragastric administration of 100 μl of normal saline; model group (10): intragastric administration of 100 μl of 2% adenine suspension every day, a total of 4 week. Bifidobacterium bifidum CCTCC M 2017063 group (10 rats): after intragastric administration of 100 μl of 2% adenine suspension daily for 1 week, resuspend Bifidobacterium bifidum CCTCC M 2017063 in 2% adenine suspension daily Suspension, respectively, 100μl orally for 3 weeks. After feeding, blood was collected from the eye sockets, and the mice were sacrificed by neck dislocation. After the plasma was allowed to stand at 4°C, the serum was collected to detect relevant biochemical indicators. The kidneys were taken for HE staining, and the degree of renal failure and improvement of the mice were observed.

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Abstract

The invention discloses a strain namely bifidobacterium bifidum CCTCC No.M2017063. The strain has a strong performance on assisting the treatment of renal diseases, is separated from feces of healthyinfants, is a gram positive polymorphic bacillus, has a strong performance on degrading serum creatinine and urea nitrogen of mice, can be made into a microbial inoculant, can be used as a ferment oradditive, and can improve the health of patients of renal diseases.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a newly isolated strain of Bifidoacferium bifidum and its probiotic function, which are mainly used for reducing creatinine and blood urea nitrogen in patients with chronic kidney disease. Background technique [0002] Kidney disease is a serious public health problem that threatens the physical and mental health of patients and brings a heavy economic burden to patients and society. At present, more than 500 million people in the world suffer from different kidney diseases, and more than one million people die from cardiovascular and cerebrovascular diseases associated with chronic kidney disease every year, while the prevalence of chronic kidney disease in the adult population in my country is 10.8% (1.2 100 million). In the advanced stage of the disease or in the uremia stage, it is extremely difficult to treat, not only to endure the pain of the disease, but also to cause an unbea...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P13/12A23C9/12A23L33/135A23L2/84C12R1/01
Inventor 徐锋蔚晓敏魏华杨栋
Owner NANCHANG UNIV
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