Engineering bacteria and application thereof

A technology for engineering strains and genetically engineered bacteria, applied in the field of bioengineering, can solve the problems of difficulty in efficiently producing tyrosol, being difficult to provide, increasing conversion costs, etc., and achieving good industrial application prospects, poor substrate specificity, and production process. simple effect
CN107586752AActive Publication Date: 2018-01-16HONGTAOSIM RES INST OF ANALYCAL SCI & TECH LTD CO
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HONGTAOSIM RES INST OF ANALYCAL SCI & TECH LTD CO
Publication Date
2018-01-16

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Abstract

The invention discloses escherichia coli gene engineering bacteria for four enzyme co-expression. The engineering bacteria is characterized by introducing an L-amino acid oxidase gene, an alpha-ketonic acid decarboxylase gene, an alcohol dehydrogenase gene, and an enzyme gene capable of reducing NAD(P) to NAD(P)H. The invention further discloses a construction method and application of recombinantescherichia coli. The engineering bacteria is applied to biological synthesis of phenylethyl alcohol compounds, has the characteristics of simple operation, low cost, high product synthetic efficiency, and high optical purity, and has bright industrial prospects.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to the construction of an Escherichia coli genetically engineered bacterium co-expressing four enzymes, and the application of the engineered bacterium in producing 2-phenylethanol, tyrosol and hydroxytyrosol. Background technique

[0002] Phenylethanol compounds (Phenylethanoids), there are three main structural analogs: 2-phenylethanol (2-Phenylethanol, β-Phenylethanol), tyrosol (4-hydroxyphenylethanol, Tyrosol, 4-hydroxyphenylethanol, 2-(4-Hydroxyphenyl) ethanol), hydroxytyrosol (3,4-dihydroxyphenylethanol, Hydroxytyrosol, 3,4-Dihydroxyphenylethanol, 2-(3,4-Dihydroxyphenyl)ethanol), are all L-α-aromatic amino acid derivatives. 2-Phenylethyl alcohol is a kind of aromatic alcohol with elegant and delicate rose fragrance. It is widely used in food, medicine, cosmetics, tobacco and daily chemical products. It is not only the basic component of all rose fragrances, ...

Claims

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