Compound bacterial agent for remediating metamitron-polluted water body

A technology of compound bacterial agent and mefenazone, applied in the field of microorganisms, can solve the problem of rare degrading bacterial agent, and achieve the effects of good synergy, improved removal rate, and high bacterial adhesion.

Active Publication Date: 2018-01-19
湖北茂盛生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few researches on bacterial agents for treating water bodies polluted by methacridone pesticide, and degrading bacterial agents are rare

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A composite bacterial agent for repairing methacridone polluted water, which is prepared according to the following steps:

[0031] Soak the polyphenylene ether in saturated saline for 2 hours, then drain the liquid, then soak it in 3wt% sodium hydroxide solution for 6 hours, drain the liquid, then rinse it with 75% ethanol until neutral, then add chitosan, glycerin and water, mix well, heat in a water bath at 60°C for 1 hour, vacuum filter, add 2% glutaraldehyde solution, cross-link for 1 hour, rinse with water to remove excess glutaraldehyde, then add 0.5% glutaraldehyde M CaCl 2 The aqueous solution was allowed to stand at room temperature for 12 hours, and dried in vacuum to obtain a carrier; the polyphenylene ether, chitosan, glycerin, water, glutaraldehyde solution and CaCl 2 The ratio of the aqueous solution is: 1kg:50g:5kg:2kg:5L:3L; the pore diameter of the polyphenylene ether is 100-300μm, and the specific surface area is 40-90m 2 / g;

[0032] Acidovorax de...

Embodiment 2

[0037] A composite bacterial agent for repairing methacridone polluted water, which is prepared according to the following steps:

[0038]Soak the polyphenylene ether in saturated saline for 2 hours, then drain the liquid, then soak it in 3wt% sodium hydroxide solution for 6 hours, drain the liquid, then rinse it with 75% ethanol until neutral, then add chitosan, glycerin and water, mix well, heat in a water bath at 60°C for 1 hour, vacuum filter, add 2% glutaraldehyde solution, cross-link for 1 hour, rinse with water to remove excess glutaraldehyde, then add 0.5% glutaraldehyde M CaCl 2 The aqueous solution was allowed to stand at room temperature for 12 hours, and dried in vacuum to obtain a carrier; the polyphenylene ether, chitosan, glycerin, water, glutaraldehyde solution and CaCl 2 The ratio of the aqueous solution is: 1-2kg:50-80g:5-7kg:2-3kg:5-7L:3-5L; the pore diameter of the polyphenylene ether is 100-300μm, and the specific surface area is 40-90m 2 / g;

[0039] A...

Embodiment 3

[0044] Test one:

[0045] Water body sample selection: Prometrione-contaminated water comes from the agricultural planting area in Hangzhou, and the concentration of fenmetrione in the water is set at 100mg / L and 1000mg / L;

[0046] Group: Example 1 composite bacterial agent is the experimental group; Control group 1: without adding Acidovorax delft, the rest is the same as in Example 1; Control group 2: without adding Pseudomonas aeruginosa, the rest is the same as in Example 1 ; Control group 3: do not add Bacillus atrophaeus, all the other are the same as embodiment 1; Control group 4: do not add Pseudomonas niger, all the other are the same as embodiment 1;

[0047] How to use: Fence intercepts to remove solid impurities, adjusts the pH of the water to 6.5-7.2, and then follows

[0048] The amount of 3g per cubic meter is added with compound bacterial agent, and the treatment time is 72 hours. See Table 1 for the content of promethazone pollutants in the discharged water ...

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Abstract

The invention belongs to the technical field of microorganisms and discloses a compound bacterial agent for remediating a metamitron-polluted water body. The compound bacterial agent is prepared by the following steps: (1) uniformly mixing a Delftia acidovorans seed solution, a Pseudomonas aeruginosa seed solution, a Bacillus atrophaeus seed solution, and a Pseudomonas black seed solution, inoculating the mixture into an inorganic salt domestication culture medium, and culturing, so as to obtain compound bacteria liquid; and (2) putting the compound bacteria liquid into a carrier of which themass is two times that of the compound bacteria liquid. The compound bacterial agent is capable of effectively remediating the metamitron-polluted water body, high in removal rate and strong in environmental friendliness.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a composite bacterial agent for repairing methacridone polluted water bodies. Background technique [0002] The chemical name of metamitron is 4-amino-3-methyl-6-phenyl-1,2,4-triazin-5(4H)-one, which belongs to triazinone selective preemergence Herbicides have a wide range of applications. They are mainly absorbed through the roots of plants and then transported to the leaves. They play a herbicidal role by inhibiting the Hill reaction of photosynthesis. It can control monocotyledonous and dicotyledonous weeds. It is a herbicide used to control gramineous and broad-leaved weeds in beet fields. It is mainly used for field weeding of dryland crops, such as corn and sugar beets. Straw, wild sesame, bluegrass and other weeds. [0003] The loss of fenmetrione pesticide into the environment will cause serious environmental pollution, and sometimes even cause extremely da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C02F101/30C12R1/385C12R1/07C12R1/38
Inventor 孙雪华秦斐潘青青
Owner 湖北茂盛生物有限公司
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