Icariin nanoparticle and preparation method thereof

An icariin and nanoparticle technology is applied in the field of icariin nanoparticle and its preparation, which can solve the problems of difficult icariin, poor oral absorption, low bioavailability and the like, and achieves simple and cheap materials and high solubility. Improved performance and good repeatability

Inactive Publication Date: 2018-02-02
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, icariin is poorly soluble in water, h

Method used

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  • Icariin nanoparticle and preparation method thereof
  • Icariin nanoparticle and preparation method thereof
  • Icariin nanoparticle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Dissolve 10 mg of icariin raw material drug in 10 ml of methanol / ethyl acetate solution (volume ratio 1:1) to prepare an icariin solution with a concentration of 1.0 mg / ml; dissolve 300 mg of PVA in 10 ml water, configure a PVA solution with a concentration of 30 mg / ml. Measure 0.75 ml of icariin solution and add it to 2.25 ml of PVA solution, and then carry out ultrasonic emulsification of the mixture; the ultrasonic frequency is 20-25 KHz, the power is 250 W, the ultrasonic time is 6 min, every ultrasonic 5 s, interval 10 s. The emulsified solution was transferred to a rotary evaporator, and the methanol, dichloromethane, and chloroform were completely evaporated by rotary evaporation at room temperature for 10 minutes, and then the sample was centrifuged at high speed for 30 minutes (4°C, 20,000 rpm) to collect the precipitate and add Resuspend in deionized water and centrifuge again. The operation was repeated 5 times, and the nanoparticles were cleaned to obtain ...

Embodiment 2

[0025] Dissolve 20 mg of icariin crude drug in 10 ml of methanol / dichloromethane solution (volume ratio of 5:1) to prepare an icariin solution with a concentration of 2.0 mg / ml; dissolve 300 mg of PVA in 10 ml water, configure a PVA solution with a concentration of 30 mg / ml. Measure 0.75 ml of icariin solution and add it to 2.25 ml of PVA solution, and then carry out ultrasonic emulsification of the mixture; the ultrasonic frequency is 20-25 KHz, the power is 100 W, the ultrasonic time is 6 min, every ultrasonic 5 s, interval 10 s. The emulsified solution was transferred to a rotary evaporator, and the methanol, dichloromethane, and chloroform were completely evaporated by rotary evaporation at room temperature for 10 minutes, and then the sample was centrifuged at high speed for 30 minutes (4°C, 20,000 rpm) to collect the precipitate and add Resuspend in deionized water and centrifuge again. The operation was repeated 5 times, and the nanoparticles were cleaned to obtain ic...

Embodiment 3

[0027] Dissolve 30 mg of icariin raw material drug in 10 ml of methanol / chloroform solution (volume ratio of 9:1), and prepare an icariin solution with a concentration of 3.0 mg / ml; dissolve 300 mg of PVA in 10 ml of water, configure a PVA solution with a concentration of 30 mg / ml. Measure 0.75 ml of icariin solution and add it to 2.25 ml of PVA solution, and then carry out ultrasonic emulsification of the mixture; the ultrasonic frequency is 20-25 KHz, the power is 400 W, the ultrasonic time is 6 min, every ultrasonic 5 s, interval 10 s. The emulsified solution was transferred to a rotary evaporator, and the methanol, dichloromethane, and chloroform were completely evaporated by rotary evaporation at room temperature for 10 minutes, and then the sample was centrifuged at high speed for 30 minutes (4°C, 20,000rpm), and the precipitate was collected and added to Resuspend in deionized water and centrifuge again. The operation was repeated 5 times, and the nanoparticles were c...

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Abstract

The invention discloses an icariin nanoparticle and a preparation method thereof. The preparation method includes preparing an icariin solution and a PVA solution, adding the icariin solution into thePVA solution, and performing emulsification after even mixing; collecting the mixed solution after emulsification, and evaporating an organic solvent; centrifuging a sample, washing sediments by purified water, performing centrifugation again, and repeating operations to wash the nanoparticle clean to obtain the icariin nanoparticle. The average fluid dynamics diameter of the obtained icariin nanoparticle is 100-400 nanometers. Compared with icariin crude drugs, the icariin nanoparticle has the advantages that water dissolving property of the icariin nanoparticle is improved by more than 10 times, and dispersing performance is also improved remarkably, so that biological utilization rate and medicinal effect of the icariin nanoparticle can be improved effectively. The preparation method does not need carriers, used materials are simple and cheap, the preparation process is simple and convenient to operate, and repeatability is high.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine pharmacy, and in particular relates to icariin nanoparticles and a preparation method thereof. technical background [0002] Icariin is an effective active ingredient extracted from the traditional Chinese medicine Epimedium. It is a flavonoid glycoside compound that has pharmacological activities such as anti-tumor, promoting bone cell proliferation and differentiation, protecting brain hypoxia, and regulating body immunity. However, icariin is poorly soluble in water, has poor oral absorption and low bioavailability. Drug absorption requires appropriate water solubility and lipid solubility in order to penetrate the lipid bilayer of biological membranes. Using modern nanotechnology to nanometerize insoluble drugs can greatly improve solubility and dispersibility, enhance biological activity and target utilization; at the same time reduce the use of organic solvents, reduce ...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/7048A61P35/00A61P19/10A61P39/00A61P37/02
CPCA61K9/14A61K31/7048A61K36/296
Inventor 姜建芳
Owner ZUNYI MEDICAL UNIVERSITY
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