Lactobacillus rhamnosus and application thereof in preparation of feed additives

A technology of Lactobacillus rhamnosus and bacterial agent, which is applied in the field of Lactobacillus rhamnosus and its application in the preparation of feed additives, can solve the problems of separation of alfalfa leaves and stems, pollution of silage raw materials, and reduction of nutritional components. Achieve the effect of improving the quality of alfalfa silage, solving the problem of silage and improving the quality of silage

Inactive Publication Date: 2018-02-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Most of the areas where alfalfa is grown in my country are Northwest, North China, Northeast and other regions. Affected by my country's monsoon climate, the rainfall in Northeast China, Huanghuaihai and Northeast China is concentrated. The harvest time of alfalfa is summer, and it is accompanied by the same rainy season. Rain during the process will lead to mildew and corruption and reduce the nutritional content, and factors such as leaf fall during the drying process will cause a large loss of alfalfa nutrients
In actual production, alfalfa has the risk of being exposed to rain whenever it is dried, and manual turning during the drying process will cause the separation of alfalfa leaves and stalks. Pollution, solving alfalfa preparation and safe storage of alfalfa in rainy season is still a difficult problem

Method used

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  • Lactobacillus rhamnosus and application thereof in preparation of feed additives
  • Lactobacillus rhamnosus and application thereof in preparation of feed additives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1, Isolation, screening and identification of Lactobacillus rhamnosus

[0048] 1. Isolation and screening of strains

[0049] Collect alfalfa silage, take 20g of alfalfa silage and add 180ml of normal saline, soak for 2h, filter the leachate, shake it with an oscillator for 30min, 10 -1 -10 -6 For gradient dilution, take 1mL from each dilution gradient dilution and inoculate it into a petri dish, add 10-15ml sterilized MRS solid medium, shake well, wait for it to cool and solidify, and let it stand for anaerobic culture for 48h. Pick single colonies with obvious differences in colony shape, size, color and gloss, and repeat streaking until pure colonies are obtained. Single colonies were picked for Gram staining and catalase test. All Gram-positive, catalase-negative strains were initially identified as lactic acid bacteria, and then a lactic acid bacterium with high growth and acid production capacity was screened out with alfalfa fresh grass extract medium,...

Embodiment 2

[0060] Embodiment 2, Lactobacillus rhamnosus prepares alfalfa silage

[0061] 1. Preparation of alfalfa silage agent

[0062] 1. Inoculate Lactobacillus rhamnosus YLGG2017 into 15ml MRS liquid medium, and culture it statically at 37°C for 48h to obtain seed solution (OD 600nm = 1.864).

[0063] 2. Inoculate 0.5 μl of the seed liquid obtained in step 1 into 15 ml of MRS liquid medium (initial OD 600nm =0.02), cultured statically at 37°C for 24 hours to obtain a bacterial suspension (OD 600nm = 2.2).

[0064] 3. Centrifuge the bacterial suspension obtained in step 2 at 8000 rpm for 15 minutes to collect the bacterial cells, and wash the bacterial cells repeatedly with physiological saline.

[0065] 4. Freeze-dry after mixing the thalline precipitation after the processing of step 3 with 10ml sterilized skim milk to obtain the alfalfa silage bacterial agent (comprising 2.3 × 10 10 CFU / g of Lactobacillus rhamnosus YLGG2017).

[0066] 2. Preparation of alfalfa silage

[0067...

Embodiment 3

[0069] Embodiment 3, the detection of alfalfa silage

[0070] Get the alfalfa silage that embodiment 2 obtains and detect as follows:

[0071] 1. Take alfalfa silage samples, dry them in an oven at 65°C for 48 hours, and calculate the water content of alfalfa silage according to the weight loss.

[0072] 2. Take alfalfa silage samples, dry them in an oven at 65°C for 48 hours, and measure the content of water-soluble carbohydrates, crude protein, reducing sugars, free amino acids, neutral detergent fiber (NDF) and acid detergent fiber (NDF) content.

[0073] Water-soluble carbohydrates were determined by anthrone-sulfuric acid colorimetry.

[0074] Crude protein was determined by the Kjeldahl method (Full-automatic Kjeldahl analyzer from FOSS Company).

[0075] The reducing sugar was determined by 3,5-dinitrosalicylic acid colorimetric method.

[0076] Free amino acids were determined by the ninhydrin colorimetric method.

[0077] Neutral detergent fiber (NDF) content and...

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Abstract

The invention discloses Lactobacillus rhamnosus and application thereof in the preparation of feed additives. The Lactobacillus rhamnosus YLGG2017 is collected under CGMCC No. 14463. The Lactobacillusrhamnosus YLGG2017 may serve as an alfalfa silage additive; the alfalfa silage additive can provide improved alfalfa silage quality under high moisture condition, the silage problem is effectively solved for high-moisture alfalfa, and silage quality is improved.

Description

technical field [0001] The invention relates to the field of silage quality and safety, in particular to a lactobacillus rhamnosus and its application in the preparation of feed additives. Background technique [0002] Alfalfa (Medicago sativa L.) is the most important leguminous forage crop in the world. It is rich in protein, vitamins and other nutrients. Macro and trace mineral elements, various vitamins and unknown growth and reproduction factors can significantly improve the digestion efficiency of herbivores, improve the yield and quality of livestock products, and play a very important role in the dairy industry. Therefore, alfalfa is also called "milk production". of the first workshop". [0003] Alfalfa is high in protein and has the reputation of the king of forage. The protein content of the alfalfa hay cut from the late budding stage to the flowering stage is more than 16%, the protein content of the alfalfa hay cut later can reach 12%-15%, and the protein dige...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18A23K10/12C12R1/225
CPCA23K10/12A23K30/18C12N1/20C12R2001/225C12N1/205A23V2400/175
Inventor 玉柱吴哲杨玉玺张英俊杨富裕
Owner CHINA AGRI UNIV
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