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Type-A baculovirus insecticide for preventing hyphantria cunea and preparation method

A technology of American white moth and baculovirus, which is applied in the direction of insecticides, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of small virus content and unsuitable production of virus insecticides, etc., and achieve a solution Effects of waste pollution, solution to UV light sensitivity, and ease of operation

Inactive Publication Date: 2018-02-23
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The virus content in these pupae is not large, so they are not suitable for the production of virus insecticides and can only be treated as waste.

Method used

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  • Type-A baculovirus insecticide for preventing hyphantria cunea and preparation method
  • Type-A baculovirus insecticide for preventing hyphantria cunea and preparation method
  • Type-A baculovirus insecticide for preventing hyphantria cunea and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] An alpha baculovirus insecticide against American white moth, comprising, per liter of insecticide:

[0031]

[0032] Described 20,000,000,000 PIB / grams of American white moth nuclear polyhedrosis virus parent drug are made by following method:

[0033] The larvae of H. americanum were fed with artificial feed, and were inoculated with H. americanum nuclear polyhedrosis virus-T98 strain (CCTCC NO:V201639) when the larvae reached the fourth instar, and the concentration of the inoculated infection solution was 6 million PIB / mL. The virus-infected larvae were cultivated under the conditions of temperature 27±1° C. and relative humidity 75-80%, and virus-killed worms were collected from the 5th day after the infection, and ended on the 10th day after the infection. The virus-killed insects were collected and processed to prepare 20 billion PIB / gram of the virus master drug, and the virus master drug prepared here was also used in Examples 2-4.

[0034] The insect-deriv...

Embodiment 2

[0038] An alpha baculovirus insecticide against American white moth, comprising, per liter of insecticide:

[0039]

[0040] The preparation method is the same as in Example 1.

[0041] The product has a good shelf life, and it can be stored for 2 years in a warehouse at room temperature, and its insecticidal activity remains above 80%.

[0042] In the comparative test of ultraviolet light irradiation, the nuclear polyhedrosis virus preparation of cotton bollworm was used as the control product. Both this product and the cotton bollworm virus insecticide were irradiated for 4 hours at a distance of 20 centimeters from a 15-watt ultraviolet lamp. The products were then removed for bioassay testing. The test results show that after the cotton bollworm virus insecticide is treated with ultraviolet light, the virus insecticide maintains 32.18% of the original activity, and after the product is treated with ultraviolet light, the virus biological activity is 59.82% of the orig...

Embodiment 3

[0044] An alpha baculovirus insecticide against American white moth, comprising, per liter of insecticide:

[0045]

[0046]

[0047] The preparation method is the same as in Example 1.

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Abstract

The invention discloses a type-A baculovirus insecticide for preventing hyphantria cunea and a preparation method. The insecticide is prepared from 20 billion PIB / g of hyphantria cunea karyotype polyhedral virus technical concentrate, insect source amino acids, tyrosine, tryptophan, fluorescent whitening powder, sodium lignin sulfonate, diflubenzuron, xanthan gum, rapeseed oil, alkyl polyglycosideand water. In view of the problem of environment pollution caused by waste due to a great number of dead moths and waste pupae during production of insect viruses, the dead moths and the waste pupaeare subjected to scaly hair removal and degreasing, and then the insect source amino acids and oligopeptide are extracted. The extraction process method of these amino acids is simple and easy to operate, and well solves the problem of waste pollution during production. The extracted insect source amino acids and oligopeptide, serving as insect virus protecting agents, are added into an insect virus insecticide preparation, so that the light protection capability of viruses can be improved, the problem of sensitivity of the insect viruses to ultraviolet light is solved, and the shelf life of avirus preparation reaches two years.

Description

technical field [0001] The invention belongs to the technical field of biological control of forestry pests, and in particular relates to a type A baculovirus insecticide for preventing and controlling white moth and a preparation method thereof. Background technique [0002] The American white moth (Hyphantria cunea Drury) belongs to the Lepidoptera and the Diphrididae family. It is an alien invasive pest and is an important quarantine object in forestry in my country and many countries in the world. Native to the United States, the worm now occurs in more than 100 countries in North America, Europe, and Asia. In 1979, the introduction of the insect was first discovered in Dandong, Liaoning, my country, and it is currently harmful in Beijing, Liaoning, Tianjin, Hebei, Shandong and other provinces and cities. American white moth is an imported quarantine pest. There is no natural enemy system in China. In addition, the insect has a variety of diets, strong adaptability, lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01N63/02A01N47/34A01N43/38A01N37/44A01N43/16A01P7/04C12N7/00
CPCA01N37/44A01N43/16A01N43/38A01N47/34A01N63/10A01N65/00A01N65/08C12N7/00C12N2710/14151A01N2300/00Y02A50/30
Inventor 张忠信孙修炼类承凤吴柳柳
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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