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Solid phase extraction column for simultaneous purification of various mycotoxins and application thereof

A solid-phase extraction column and mycotoxin technology, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of poor enrichment and purification effect, undetectable, complicated reagents, etc., and achieve convenient pre-treatment, reduce consumption, and prepare Effects in simple steps

Active Publication Date: 2018-02-23
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to solve the problem that the existing solid-phase extraction column technology has poor detection, enrichment and purification effects on various mycotoxins in agricultural products, low recovery rate, and relatively complicated reagents, which cannot accurately detect various fruits, vegetables, grains and food samples. detection problem, and then proposed a solid-phase extraction column for the simultaneous purification of multiple mycotoxins and its application

Method used

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  • Solid phase extraction column for simultaneous purification of various mycotoxins and application thereof
  • Solid phase extraction column for simultaneous purification of various mycotoxins and application thereof
  • Solid phase extraction column for simultaneous purification of various mycotoxins and application thereof

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Effect test

Embodiment 1

[0041] Embodiment 1: raw material and preparation of solid phase extraction column

[0042] 1. Raw materials:

[0043] (1) Column tube: 6mL polypropylene or glass column tube with an inner diameter of 12.7mm;

[0044] (2) Sieve plate: polyethylene or polytetrafluoroethylene sieve plate with a pore size of 10-30 μm;

[0045] (3) Carboxylated multi-walled carbon nanotube filler: 10 mg of carboxylated multi-walled carbon nanotube filler with a length of 50 μm;

[0046] (4) Reversed-phase chromatography packing: 200 mg of reverse-phase chromatography packing C with a particle size of 40-60 μm 18 ;

[0047] 2. Assembly method (see attached figure 1 )

[0048] Carboxylated multi-walled carbon nanotubes (attached figure 1 4) and reversed-phase chromatography packing (attached figure 1 3) After mixing uniformly according to a certain ratio to obtain the compound packing, pour it into polypropylene or glass column tube (attached figure 1 In step 1), first fill a piece of sieve ...

Embodiment 2

[0049] Embodiment 2: raw material and preparation of solid phase extraction column

[0050] 1. Raw materials

[0051] (1) Column tube: 6mL polypropylene or glass column tube with an inner diameter of 12.7mm;

[0052] (2) Sieve plate: polyethylene or polytetrafluoroethylene sieve plate with a pore size of 10-30 μm;

[0053] (3) Carboxylated multi-walled carbon nanotube filler: 20 mg of carboxylated multi-walled carbon nanotube filler with a length of 50 μm;

[0054] (4) Reversed-phase chromatography packing: 200 mg of reverse-phase chromatography packing C with a particle size of 40-60 μm 18 ;

[0055] 2. Assembly method

[0056] First mix carboxylated multi-walled carbon nanotubes and reversed-phase chromatographic fillers in a certain proportion to obtain a compound filler, then fill a piece of sieve plate into a polypropylene or glass column tube, add the mixed compound filler, and then fill Insert the second sieve plate and press firmly.

Embodiment 3

[0057] Embodiment 3: raw material and preparation of solid phase extraction column

[0058] 1. Raw materials

[0059] (1) Column tube: 6mL polypropylene or glass column tube with an inner diameter of 12.7mm;

[0060] (2) Sieve plate: polyethylene or polytetrafluoroethylene sieve plate with a pore size of 10-30 μm;

[0061] (3) Carboxylated multi-walled carbon nanotube filler: 20 mg of carboxylated multi-walled carbon nanotube filler with a length of 50 μm;

[0062] (4) Reverse-phase chromatography filler: 200 mg reverse-phase chromatography filler HLB with a particle size of 40-60 μm;

[0063] 2. Assembly method

[0064] First mix carboxylated multi-walled carbon nanotubes and reversed-phase chromatographic fillers in a certain proportion to obtain a compound filler, then fill a piece of sieve plate into a polypropylene or glass column tube, add the mixed compound filler, and then fill Insert the second sieve plate and press firmly.

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Abstract

The invention provides a solid phase extraction column for simultaneous purification of various mycotoxins and an application thereof. The solid phase extraction column comprises multi-walled carbon nanotubes and a reversed-phase chromatography filler. Through compounding of the multi-walled carbon nanotubes and the reversed-phase chromatography filler, simultaneous one-step purification of various mycotoxins is effectively realized, a cost and analysis time are saved and the solid phase extraction column is suitable for various fruits, vegetables, grains, feed and food samples. The solid phase extraction column is especially suitable for simultaneous purification of various fusarium toxins.

Description

technical field [0001] The invention relates to the technical field of agricultural product safety detection, in particular to a solid-phase extraction column for simultaneously purifying multiple mycotoxins and an application thereof. Background technique [0002] Mycotoxins are toxic metabolites produced by toxin-producing fungi under suitable environmental conditions, which can widely contaminate plant-derived products such as crops, food, and feed. At present, more than 400 kinds of mycotoxins have been found in nature, among which the most concerned are aflatoxin (Aflatoxin, AFT), deoxynivalenol (Deoxynivalenol, DON), T-2 toxin, zearalenone (Zearalenone, ZEN), fumonisin (Fumonisin, FB), ochratoxin (Ochratoxin, OTA), tenuazonic acid (TeA), etc. Mycotoxin contamination of food not only causes huge economic losses, but also causes human poisoning, nausea, vomiting, abdominal distension, abdominal pain in mild cases, and coma or even death in severe cases, posing a great t...

Claims

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Application Information

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IPC IPC(8): G01N30/60G01N30/14
CPCG01N30/14G01N30/60
Inventor 王蒙冯晓元韦迪哲田晓琴姜冬梅王刘庆姜楠
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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