Two-phase deep-eutectic solvent capable of extracting active components of ginkgo leaves, and preparation method and extraction method of two-phase deep-eutectic solvent

A co-melting solvent and active ingredient technology, applied in the field of utilization of agricultural and forestry biological resources, can solve the problems of waste of biological active ingredients, environmental and human hazards, increase extraction steps, etc., and achieve low cost, good fluidity, and low vapor pressure.

Active Publication Date: 2018-03-13
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, water or organic solvents are usually used as extraction solvents for the extraction of active components of ginkgo biloba. Water as an extraction solvent has poor extraction effect, while traditional organic solvents are volatile and easy to remain, causing harm to the environment and human body, and high cost
In addition, since ginkgo flavonoids, terpene lactones, and proanthocyanidins are hydroph

Method used

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  • Two-phase deep-eutectic solvent capable of extracting active components of ginkgo leaves, and preparation method and extraction method of two-phase deep-eutectic solvent
  • Two-phase deep-eutectic solvent capable of extracting active components of ginkgo leaves, and preparation method and extraction method of two-phase deep-eutectic solvent
  • Two-phase deep-eutectic solvent capable of extracting active components of ginkgo leaves, and preparation method and extraction method of two-phase deep-eutectic solvent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1

[0048] (1) Weigh choline chloride and levulinic acid at a molar ratio of 1:2 and mix them evenly. Heat and stir at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES1. Combine DES1 with water. Mix according to the mass ratio of 6:4 to obtain substance A;

[0049] (2) Weigh choline chloride and malonic acid at a molar ratio of 1:2 and mix them evenly. Heat and stir at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES2. Combine DES2 with water. Mix according to the mass ratio of 4.5:5.5 to obtain substance B;

[0050] (3) Mix substance A and substance B at a volume ratio of 8.75:1.25 to obtain the hydrophilic phase of the two-phase deep co-solvent;

[0051] (4) At a molar ratio of 1:2:3, weigh methyl trioctyl ammonium chloride, octanol and octanoic acid and mix them evenly. Heat at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES3 , As the hydrophobic phase of the two-phase deep c...

Example Embodiment

[0053] Example 2

[0054] (1) The ratio of moles is 1:2.5. Weigh choline chloride and levulinic acid and mix them evenly. Heat and stir at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES1. Combine DES1 with water Mix according to the mass ratio of 6:4.5 to obtain substance A;

[0055] (2) Weigh choline chloride and malonic acid at a molar ratio of 1:2.5 and mix them evenly. Heat and stir at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES2. Combine DES2 with water. Mix according to the mass ratio of 4.5:6 to obtain substance B;

[0056] (3) Mix substance A and substance B in a volume ratio of 7.75:2.25 to obtain the hydrophilic phase of the two-phase deep co-solvent;

[0057] (4) At a molar ratio of 1:2:3.5, weigh the methyl trioctyl ammonium chloride, octanol and octanoic acid and mix them evenly. Heat at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES3 , As the hydrophobic phase of the two-...

Example Embodiment

[0059] Example 3

[0060] (1) The ratio of moles is 1:1.5. Weigh choline chloride and levulinic acid and mix them evenly. Heat and stir at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES1. Combine DES1 with water Mix according to the mass ratio of 6:3.5 to obtain substance A;

[0061] (2) Weigh choline chloride and malonic acid at a molar ratio of 1:1.5 and mix them evenly. Heat and stir at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES2. Combine DES2 with water Mix according to the mass ratio of 4.5:5 to obtain substance B;

[0062] (3) Mix substance A and substance B at a volume ratio of 8.75:1.25 to obtain the hydrophilic phase of the two-phase deep co-dissolving solvent;

[0063] (4) At a molar ratio of 1:2:2.5, weigh the methyl trioctyl ammonium chloride, octanol and octanoic acid and mix them evenly. Heat at 80~110℃ until a transparent liquid is formed, which is the deep co-solvent DES3 , As the hydrophobic phase o...

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Abstract

The invention discloses a two-phase deep-eutectic solvent capable of extracting active components of ginkgo leaves, and a preparation method and an extraction method of the two-phase deep-eutectic solvent. The two-phase deep-eutectic solvent consists of a hydrophilic phase and a hydrophobic phase with the same volume, wherein the hydrophilic phase consists of a substance A and a substance B; the substance A consists of DES1 and water; the DES1 is prepared from choline chloride and levulinic acid; the substance B consists of DES2 and water; the DES2 is prepared from choline chloride and propandioic acid; and the hydrophobic phase is prepared from methyl trioctyl ammonium chloride, octanol and octanoic acid. The prepared two-phase deep-eutectic solvent is a green solvent and is safe, pollution-free, low in viscosity, stable in hydrogen bond and high in flowability; and the hydrophobic active component polyisopentene alcohol acetate and the hydrophilic active components such as ginkgo flavones, terpene lactone and procyanidine can be extracted from the ginkgo leaves simultaneously, the extraction rate is high, the use safety is achieved and residues are avoided. The preparation methodof the two-phase deep-eutectic solvent is simple and convenient, low in cost and high in biodegradability.

Description

technical field [0001] The invention relates to the utilization of agricultural and forestry biological resources, in particular to a two-phase deep eutectic solvent for extracting ginkgo biloba active components, a preparation method and an extraction method thereof. Background technique [0002] Ginkgo biloba (scientific name: Ginkgo biloba L.), also known as Gongsun tree and ginkgo, belongs to gymnosperm, Ginkgo family Ginkgo genus, single genus and single species. Ginkgo biloba, fruit, etc. have medicinal development value, and are known as living fossils full of treasures. Ginkgo is native to my country, and my country's ginkgo resources account for more than 70% of the world's total. [0003] The active ingredients of Ginkgo biloba mainly include ginkgo flavonoids, terpene lactones, proanthocyanidins, polyprenyl alcohol, etc. The content of flavonoids in Ginkgo biloba ranks first, accounting for about 2.5-5.9% of the total content. More than 40 kinds of flavonoids ha...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61K127/00
CPCA61K36/16A61K2236/333A61K2236/53
Inventor 苏二正曹君李默涵曹福亮陈璐瑶赵林果王耀松
Owner NANJING FORESTRY UNIV
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