Preparation method of full shrimp cream by debitterized enzymolysis of marine small opossum shrimps and its application
A technology of mysid and debittering enzyme, which is applied in the field of protein enzymatic hydrolysis, can solve the problems of activated carbon only decolorization and high cost of ultrafiltration membrane, and achieve the effect of retaining nutrients, reducing separation cost and shortening the process flow
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Embodiment 1
[0066] A method for preparing whole shrimp paste rich in small peptides by enzymatic hydrolysis of marine mysis, comprising the following steps:
[0067] 1) Granulation, fresh seafood Mysis shrimp is used as raw material, freeze-dried to a water content of 50wt%-70wt%, graded and crushed to protein particles below 3mm;
[0068] 2) Micronization, add pure water at 0-3°C to the protein granules according to the mass ratio of protein granules and water 1:0.2, shear at ultra-high speed to a protein granule slurry above 150 mesh, add pure water to the mass ratio of raw shrimp and water 1:0.5-5, put the protein particle slurry into the holding tank; preferably, the mass ratio of feed to water is 1:0.5-2.
[0069] Specifically, the rotation diameter of the shearing blade is 20-40 mm, the motor speed is above 30,000 revolutions, a conical gathering cover is arranged around the blade, and the inner wall of the conical cover is provided with collision ribs at intervals, and the convex r...
Embodiment 2
[0097] like figure 2 , 3 As shown, in step 4), the preform is a film 5.2, and a plurality of isolation ribs 5.3 are arranged at intervals on the upper surface of the film 5.2, and the isolation ribs 5.3 are sinusoidally spaced at the same period. The upper and lower surfaces of the film 5.2 are uniformly bonded with dispersed carriers 5.4, such as silicon nuclease particles, and the concentration of the immobilized enzyme is determined by the biquinoline formic acid method to reach 2-3 μg / cm 2 , Practice has proved that the immobilized enzyme has good activity. The film 5.2 is wound around the outer peripheral surface of the shaft 5.1 to form a pre-rolled body 5 with a certain diameter, and the pre-rolled body 5 is fixed in the cylindrical immobilized enzyme reactor 1 . Since the preset winding body 5 has a tighter interval, a sinusoidal sinusoidal passage is provided between the spacer rib 5.3 and the upper and lower films, and the sinusoidal passage is opened from one end...
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