Preparation method of full shrimp cream by debitterized enzymolysis of marine small opossum shrimps and its application

A technology of mysid and debittering enzyme, which is applied in the field of protein enzymatic hydrolysis, can solve the problems of activated carbon only decolorization and high cost of ultrafiltration membrane, and achieve the effect of retaining nutrients, reducing separation cost and shortening the process flow

Active Publication Date: 2018-03-23
浙江五丰海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when producing fish / shrimp pulp by enzymatic hydrolysis, first of all, there is no need to remove color, activated carbon can only remove color, and the ultrafiltration membrane is too fine and the cost is high, so ultrafiltration membrane and activated carbon are not suitable

Method used

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  • Preparation method of full shrimp cream by debitterized enzymolysis of marine small opossum shrimps and its application
  • Preparation method of full shrimp cream by debitterized enzymolysis of marine small opossum shrimps and its application
  • Preparation method of full shrimp cream by debitterized enzymolysis of marine small opossum shrimps and its application

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Experimental program
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Embodiment 1

[0066] A method for preparing whole shrimp paste rich in small peptides by enzymatic hydrolysis of marine mysis, comprising the following steps:

[0067] 1) Granulation, fresh seafood Mysis shrimp is used as raw material, freeze-dried to a water content of 50wt%-70wt%, graded and crushed to protein particles below 3mm;

[0068] 2) Micronization, add pure water at 0-3°C to the protein granules according to the mass ratio of protein granules and water 1:0.2, shear at ultra-high speed to a protein granule slurry above 150 mesh, add pure water to the mass ratio of raw shrimp and water 1:0.5-5, put the protein particle slurry into the holding tank; preferably, the mass ratio of feed to water is 1:0.5-2.

[0069] Specifically, the rotation diameter of the shearing blade is 20-40 mm, the motor speed is above 30,000 revolutions, a conical gathering cover is arranged around the blade, and the inner wall of the conical cover is provided with collision ribs at intervals, and the convex r...

Embodiment 2

[0097] like figure 2 , 3 As shown, in step 4), the preform is a film 5.2, and a plurality of isolation ribs 5.3 are arranged at intervals on the upper surface of the film 5.2, and the isolation ribs 5.3 are sinusoidally spaced at the same period. The upper and lower surfaces of the film 5.2 are uniformly bonded with dispersed carriers 5.4, such as silicon nuclease particles, and the concentration of the immobilized enzyme is determined by the biquinoline formic acid method to reach 2-3 μg / cm 2 , Practice has proved that the immobilized enzyme has good activity. The film 5.2 is wound around the outer peripheral surface of the shaft 5.1 to form a pre-rolled body 5 with a certain diameter, and the pre-rolled body 5 is fixed in the cylindrical immobilized enzyme reactor 1 . Since the preset winding body 5 has a tighter interval, a sinusoidal sinusoidal passage is provided between the spacer rib 5.3 and the upper and lower films, and the sinusoidal passage is opened from one end...

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Abstract

The invention discloses a preparation method of full shrimp cream by debitterized enzymolysis of marine small opossum shrimps, which includes steps of 1), prilling, taking full fishes of fresh marinesmall trashes are raw material fishes; grading and smashing the fishes to acquire protein grains of which maximum dimension is less than 3mm; 2), micronization, performing superspeed shearing to obtain more than 150 meshes of protein particle slurry; 3), minimizing: boiling for 10-20 minutes at 90-100 DEG C; cooling until the micronization temperature is 55-75 DEG C; making an ultrasound probe extend to a holding slot to minimize the ultrasound wave so as to obtain tiny grain protein activating slurry; 4), enzymolysis: encircling and heating the slurry by microwave until the enzymolysis reaction temperature is 40-65 DEG C; adding 0.01-2% of protease by weight of raw material shrimp in the particle protein slurry; circularly and alternatively applying microwave, ultrasound wave and enzymatic hydrolysis for 10-20 minutes / time to obtain enzymolysis protein slurry, wherein the total enzymatic hydrolysis is 20-60 minutes;; performing enzyme deactivation, vacuum-concentrating until the watercontent is 40-60wt%, so as to obtain the full shrimp cream by debitterized enzymolysis of marine small opossum shrimps. The preparation method can replace shrimp flour as the feed protein source of economical feed for aquaculture.

Description

technical field [0001] The invention relates to the technical field of protein enzymolysis, in particular to a method for preparing whole shrimp paste rich in small peptides by enzymatic hydrolysis of marine mysis and its application. Background technique [0002] An important and costly raw material in fish and shrimp feed is protein. A variety of marine fish is the main target of proteolysis. After marine proteolysis, one of the hydrolyzed products is small peptides. Small peptides should be the general term for small molecular peptides, including molecular small peptides with 2 to 3 amino acid residues, and oligopeptides with less than 10 amino acid residues. The left and right small molecule and small peptide products can be classified as small peptide products. Small peptides have good functional properties, not only can be quickly absorbed by organisms in a complete form, directly participate in the synthesis of tissue proteins, but also have physiological functions ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20A23K10/22A23K50/80
CPCA23K10/22A23K50/80A23L5/25Y02A40/818
Inventor 许剑彬孙姜许福土王灵华
Owner 浙江五丰海洋生物科技有限公司
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