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Bioactive polypeptide QSLTLTDVE, preparation method thereof and application of bioactive polypeptide

A technology of biologically active polypeptides and derivatives, applied in the protein field, can solve the problems of poor digestibility and absorption, no public performance, etc., and achieve the effects of reducing the incidence of the body, improving the vitality, and reducing the aging of the body.

Active Publication Date: 2018-03-23
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The β-casein-H disclosed in this patent is a macromolecular protein with poor digestibility and absorption
At the same time, the patent discloses that the protein has excellent dietary functions, but does not disclose whether it has other properties

Method used

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  • Bioactive polypeptide QSLTLTDVE, preparation method thereof and application of bioactive polypeptide
  • Bioactive polypeptide QSLTLTDVE, preparation method thereof and application of bioactive polypeptide
  • Bioactive polypeptide QSLTLTDVE, preparation method thereof and application of bioactive polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The artificial synthesis of embodiment 1 active peptide QSLTLTDVE

[0040] 1. Synthesis of Bioactive Peptides

[0041] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0042] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0043] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0044] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0045] 5. ...

Embodiment 2

[0081] Anti-inflammatory activity experiment of embodiment 2 bioactive peptides

[0082] 1. Experiment of the bioactive peptide QSLTLTDVE promoting macrophages to secrete cytokines (ELISA method)

[0083] 1. Experimental reagents and instruments:

[0084]Reagents: experimental animal balb / c mouse (male 6-8 weeks old), Shanghai Slack Experimental Animal Co., Ltd.; mouse lymphocyte extract, Shanghai Suo Laibao Biotechnology Co., Ltd.; RPMI1640 medium, GIBCO company; bovine Serum albumin (bovine serum albumin, BSA), Genebase Company; milk-derived bioactive peptide QSLTLTDVE obtained in Example 1; ELISA cytokine rapid kit (TNF-α, IL-1β and IL-6), Wuhan Boster Bioengineering Ltd.

[0085] Instruments and equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO2 incubator Heraeus; Dragon WellscanMK3 microplate reader Labsystems.

[0086] 2. Experiment...

Embodiment 3

[0105] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0106] 1. The effect of biologically active polypeptide QSLTLTDVE on the structure of spleen tissue in vivo

[0107] 1. Experimental reagents and instruments:

[0108] Reagents: experimental animal ICR mice (male 5 weeks old), Shanghai Experimental Animal Center; D-gal, Sinopharm Chemical Reagent Co., Ltd.; paraformaldehyde, Sinopharm Chemical Reagent Co., Ltd.; sodium chloride, Sinopharm Chemical Reagent Co., Ltd. Co., Ltd.; the milk-derived bioactive polypeptide QSLTLTDVE obtained in Example 1.

[0109] Instruments and equipment: CM-230 Mole ultra-clean water, Shanghai Moller Scientific Instrument Co., Ltd.; MilliporeMILLEX GP0.22μm filter membrane, Millipore Corporation; GL-22M high-speed refrigerated centrifuge, Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.

[0110] 2. Experimental method:

[0111] (1) Modeling of animal aging models

[0112] After one week of adaptive feeding, the ICR mice...

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Abstract

The invention relates to the field of proteins, in particular to a bioactive polypeptide QSLTLTDVE, a preparation method thereof and an application of the bioactive polypeptide. The amino acid sequence of the bioactive polypeptide QSLTLTDVE is Gln-Ser-Leu-Thr-Leu-Thr-Asp-Val-Glu. In-vitro anti-inflammatory activity experiments and in-vivo anti-aging experiments prove that the polypeptide QSLTLTDVEhas good anti-inflammatory activity and anti-aging activity, on one hand, the bioactive polypeptide QSLTLTDVE can promote macrophage secretory cell factors, promote increase of macrophage nitric oxide inducting amount, improve external pathogen infection resistance of organisms and reduce morbidity of the organisms, on the other hand, activity of an in-vivo peroxidase resistance system can be improved, the exogenous stimulation resisting function of the organisms is enhanced, so that the probability of aging, senescence and sickness is reduced, and the bioactive polypeptide is of great importance for development of food, health care products and medicines with anti-inflammatory functions and anti-aging functions.

Description

technical field [0001] The invention relates to the field of protein, in particular to a biologically active polypeptide QSLTLTDVE and its preparation method and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] It is especially important to find safe bioactive peptides in natural food sources. In recent years, it has been found that some food-derived polypeptides have good biological activity, such as corn short peptides, soybean peptides, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A61K38/17A61P29/00A61P39/06A23L33/18
CPCA23L33/18A23V2002/00A61K38/00C07K14/4732A23V2200/30A23V2200/302A23V2250/55
Inventor 张少辉张伯宇范梦珠李云飞李婉如汪超李阜烁林学海
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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