Furans fluorescence enhanced probe for visual detection of zinc ions, preparation method and purpose thereof
A technology of fluorescence enhancement and zinc ions, which is applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., can solve the problems of spectrum and instrument interference, complex synthesis steps, poor water solubility, etc., and achieve good selectivity and sensitivity. Strong interference ability and good stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] The synthesis of embodiment 1 probe ZY10
[0032] The synthetic route of probe ZY10 is as follows:
[0033]
[0034] Specific steps are as follows:
[0035] (1) Add 3.34g (0.035mol) of guanidine hydrochloride, 5.256g (0.105mol) of hydrazine hydrate and 20mL of 1,4-dioxane into a round-bottomed flask, stir electromagnetically, and wait until the solid reactants are completely dissolved , Refluxed at 105°C for 4h. After the reaction, the reaction solution was cooled to room temperature, vacuum filtered to obtain a purple solid, and then recrystallized with 20 mL of absolute ethanol to obtain a white crystal, and intermediate 1, namely aminoguanidine hydrochloride, was obtained. 3.16 grams, the yield was 82% , m.p. 215-216°C.
[0036] (2) Dissolve 1.4g (0.010mol) of intermediate 1, aminoguanidine hydrochloride, in 3mL of water, then add 3.36g (0.035mol) of furfural and 15mL of ethanol, and reflux at 85°C for 4h. After the reaction was complete, cool to room temperat...
Embodiment 2
[0037] The selectivity research of embodiment 2 probe ZY10 to different metal ions
[0038] The probe ZY10 prepared in Example 1 is dissolved in ethanol, and it is prepared into a probe mother liquor with a concentration of 1mmol / L; distilled water is used as a solvent to prepare various concentrations of the same detection species (K + , Ca 2+ , Na + , Mg 2+ ,Zn 2+ ,Cr 3+ , Fe 3+ , Fe 2+ , Ni 2+ ,Co 2+ ,Mn 2+ ,Cu 2+ ,Pb 2+ ,Hg 2+ , Ag + ,Cd 2+ ) stock solution. Use a pipette gun to add probes and metal ions with a substance ratio of 1:4, and then dilute with ethanol and double distilled water (V EtOH :V H2O =1:19), so that the final concentration of probe and metal ion in the fluorescence cuvette is 10 μmol / L and 40 μmol / L respectively. The prepared solution was shaken, reacted for 30min, and the excitation wavelength of the probe was 294nm, and then their fluorescence spectra were tested. Depend on figure 1 It can be seen that the fluorescence of the probe...
Embodiment 3
[0039] Example 3 Different detection species recognize Zn to probe ZY10 2+ interference research
[0040] Add various competing species (K + , Ca 2+ , Na + ,Mg 2+ ,Cr 3+ , Fe 3+ , Fe 2+ , Ni 2+ ,Co 2+ ,Mn 2+ ,Cu 2+ ,Pb 2+ , Hg 2+ , Ag + ,Cd 2+ ), then diluted with ethanol and double distilled water (V EtOH :V H2O =1:19), so that the final concentrations of the probe and competing species in the fluorescence cuvette were 10 μmol / L and 100 μmol / L, respectively. The prepared solution was mixed evenly, then shaken for 30min, and tested for fluorescence intensity. Then add the same concentration of Zn as the competing species 2+ , to detect the fluorescence intensity after the competition reaction. Such as figure 2 As shown, relative to the blank test solution (no competing species), various competing species will not significantly affect the probe on Zn 2+ detection, indicating that the probe ZY10 on Zn 2+ The identification has good anti-interference abilit...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More