Preparation of oxalate decarboxylase as well as product and application of oxalate decarboxylase

An oxalate decarboxylase, acid-induced technology, applied in the directions of application, medical preparations containing active ingredients, biochemical equipment and methods, etc., can solve the problems of unindustrialization, low normal expression of oxalate decarboxylase, etc., and reduce food consumption. The effect of oxalic acid absorption and prevention of calcium oxalate urinary tract stones

Inactive Publication Date: 2018-04-03
WUHAN KANGFUDE BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem in the prior art that the normal expression of oxalate decarboxylase in edible fungus of the genus Acromanus genus oxalate decarboxylase is low and cannot be industrialized, the present invention provides a preparation method of oxalate decarboxylase, and also provides products prepared by the method and its application

Method used

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  • Preparation of oxalate decarboxylase as well as product and application of oxalate decarboxylase
  • Preparation of oxalate decarboxylase as well as product and application of oxalate decarboxylase
  • Preparation of oxalate decarboxylase as well as product and application of oxalate decarboxylase

Examples

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Effect test

Embodiment 1

[0035] Example 1: Effects of different induction methods on the production of oxalate decarboxylase

[0036] This embodiment illustrates the impact of different induction methods on the enzyme production of the oxalate decarboxylase of the fungus of the genus Amanita genus, and the specific operations are as follows:

[0037] Tea Tree Mushroom was used as the strain to culture and produce oxalate decarboxylase in shake flasks. The medium formula was: yeast powder, 4g / L; soybean peptone, 3g / L; KH 2 PO 4 , 2g / L; MgSO 4 ·7H 2 O, 0.2g / L; CaCl 2 , 1g / L; Glucose, 20g / L; Corn starch, 10g / L; Vitamin B 1 10mg / L; pH value 5.0-6.0; shake bottle liquid volume is 20-30%, sterilized at 121°C for 30 minutes (vitamin B 1 For filter sterilization, add to culture medium before inoculation). Inoculate the mycelium of tea tree mushroom cultured on the PDA plate into 3 groups (6 shake flasks) of sterilized liquid culture medium respectively, the inoculum amount remains the same, and the cult...

Embodiment 2

[0040] Example 2: Effect of Bacillus Control on Enzyme Activity

[0041] This example provides the effect of the control of mycospheres on the production of oxalate decarboxylase enzyme activity during the fermentation process by comparing the fermentation of two 7L fermenters. Specific steps are as follows:

[0042] 1) Take tea tree mushroom as the strain, use shake flask to cultivate the seed solution, and divide it into two parts. Seed medium: yeast powder, 4g / L; soybean peptone, 6g / L; KH 2 PO 4 , 1g / L; MgSO 4 ·7H 2 O, 0.5g / L; CaCl 2 , 0.1g / L; Glucose, 20g / L; Corn starch, 10g / L; Vitamin B 1 10mg / L; pH value 5.0-6.0; shake flask filling volume 20-30%, culture conditions: 23-28°C, 100rpm-350rpm for 3 days as seed solution. When a seed liquid is subcultured and amplified, use a hand-held emulsifier to crush the fungus balls; when the other seed liquid is amplified, transfer directly without crushing the emulsifier. The seed liquid is transferred to the fermentation med...

Embodiment 3

[0049] This embodiment provides a method for preparing enzyme powder and edible fungus powder containing oxalate decarboxylase, and the specific steps are as follows:

[0050] 1) Use tea tree mushroom as strain, adopt shake flask to cultivate seed solution, seed medium is: yeast powder, 4g / L; soybean peptone, 3g / L; KH 2 PO 4 , 2g / L; MgSO 4 ·7H 2 O, 0.2g / L; CaCl 2 , 1g / L; Glucose, 20g / L; Corn starch, 10g / L; Vitamin B 1 10mg / L; pH value 5.0~6.0; shake flask filling volume is 20~30%, culture conditions: 23~28℃, 100rpm~350rpm for 2~5 days, when the seed liquid is subcultured and enlarged, the method of mechanical crushing is adopted Break the bacterium ball and transfer it to the next-level seed medium with an inoculation amount of 10% to 30%;

[0051] 2) The mycelial balls of the seed liquid are mechanically crushed and inoculated into a 7L mechanically stirred fermenter with an inoculum size of 10% to 30% for fermentation. The liquid fermentation medium includes the followi...

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Abstract

The invention discloses a preparation method of oxalate decarboxylase. According to the method, via biological fermentation technical means, acid-induced expression is carried out under a condition of0.001-50mM of manganese ions, so that the oxalate decarboxylase is produced. The invention also discloses a preparation prepared by the method and an application of the preparation. With the application of the method provided by the invention, the efficient preparation of the oxalate decarboxylase can be achieved.

Description

technical field [0001] This application relates to the preparation of an oxalate decarboxylase, its preparation and application. technical background [0002] Oxalic acid (oxalic acid) is a molecular formula C 2 o 4 h 2 , a dibasic organic acid with a molecular weight of 90.04, which exists in most common foods, and is very high in specific foods (such as spinach or other green vegetables, bamboo shoots, green tea, cocoa beans, soybean milk, and coffee, etc.). Oxalic acid is the final product in the body and cannot be further decomposed. The main way of excretion is to excrete it with urine. Oxalic acid in the human body includes exogenous oxalic acid and endogenous oxalic acid. Exogenous oxalic acid refers to oxalic acid ingested through diet, and endogenous oxalic acid refers to oxalic acid produced by the human liver itself. Exogenous oxalic acid in people with a high risk of kidney stones is much greater than endogenous oxalic acid. [0003] When the oxalic acid con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12N1/14A23L31/00A23L33/10A23K10/30A23K20/189A61K38/51A61P13/04A61P13/00C12R1/645
CPCA23V2002/00A61K38/00A61K38/51A23K10/30A23K20/189A23L29/06A23L31/00A23L33/10C12N1/14C12N9/88C12Y401/01002A23V2200/30A23V2250/208A23V2300/14A61P13/00A61P13/04
Inventor 宋保平刘海峰陈火晴陈先桥童佩汪小锋
Owner WUHAN KANGFUDE BIOTECH CO LTD
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