A method for increasing antigen excess and linear range of latex immunoturbidimetric assays

A technology of latex immunity and linear range, applied in the direction of material inspection products, biological testing, etc., can solve the problem of high cost, achieve the effect of increasing raw material cost, wide application range, and reducing reagent cost

Active Publication Date: 2019-06-28
BYRON DIAGNOSTICS SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this solution encounters the problem of individually optimizing the antibody production process for each different project, and the cost remains high

Method used

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  • A method for increasing antigen excess and linear range of latex immunoturbidimetric assays
  • A method for increasing antigen excess and linear range of latex immunoturbidimetric assays
  • A method for increasing antigen excess and linear range of latex immunoturbidimetric assays

Examples

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Embodiment 1

[0044] A method for improving the antigen excess and linear range of the latex immunoturbidimetric method in this embodiment is applied to a urine microalbumin detection kit (latex immunoturbidimetric method), abbreviated as Malb. Include the following steps:

[0045] Step 1, use the CB0225D latex solution of Polymicrospheres company of 500ml, comprise the latex that the particle diameter of 10% mass concentration is 225nm, add distilled water to carry out cross-flow filtration through the 50KD cut-off pore size ultrafiltration membrane bag of Pall company, complete 16 times of liquid change, Remove residual styrene ST and carboxyl monomers.

[0046] Step 2, adding the latex solution after the replacement of distilled water into a four-hole reaction kettle with a capacity of 1000ml, adding 1.5g lithium chloride and 0.4g sodium dodecylbenzenesulfonate, the reaction kettle is placed in a constant temperature water bath, and kept Constant temperature of 55°C, mechanical stirring...

Embodiment 2

[0067] A method of increasing the antigen excess and linear range of the latex immunoturbidimetric method in this embodiment is applied to a C-reactive protein detection kit (latex immunoturbidimetric method), abbreviated as CRP. Include the following steps:

[0068] Step 1. Use 500ml of Agilent’s product number PL6115 latex solution, including 10% mass concentration of latex with a particle size of 138nm, through Millipore’s 50KD cut-off pore size ultrafiltration membrane bag, add distilled water for cross-flow filtration, and complete an 8-fold liquid change .

[0069] Step 2, adding the latex solution after the replacement of distilled water into a four-hole reaction kettle with a capacity of 1000ml, adding 1.5g lithium chloride and 0.4g sodium dodecylbenzenesulfonate, the reaction kettle is placed in a constant temperature water bath, and kept Constant temperature of 50°C, mechanical stirring at 260rpm for 10min. Then, after argon gas was passed to remove oxygen for 5 mi...

Embodiment 3

[0086] A method of increasing the antigen excess and linear range of the latex immunoturbidimetric method in this embodiment is applied to the alpha-fetoprotein detection kit (latex immunoturbidimetric method), abbreviated as AFP. This reagent uses two secondary convergent polymer latexes with different particle sizes, and uses the conventional technique of mixing two types of latex, one large and one small, to take into account sensitivity and linear range.

[0087] 1. Preparation of small latex reagent 2:

[0088] Step 1. Use 500ml of JSR company's no-clean latex solution, including 5% mass concentration of latex with a particle size of 157nm, and add distilled water to perform cross-flow filtration through Pall's 50KD cut-off pore size ultrafiltration membrane bag to complete a 2-fold liquid change .

[0089] Step 2, adding the latex solution after the replacement of distilled water into a four-hole reaction kettle with a capacity of 1000ml, adding 0.75g lithium chloride a...

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Abstract

The invention discloses a method for improving latex immune turbidity method antigen excess and linearity range. The method comprises the steps of firstly, adding distilled water into a latex solutionto perform cross flow filtration; secondly, adding the latex solution after replacing the distilled water into a reaction pot, adding lithium chloride and sodium dodecyl benzene sulfonate and stirring evenly, then inletting argon or nitrogen, eliminating oxygen, adding potassium peroxodisulfate, and triggering convergence polymerization reaction in a microsphere for the second time; thirdly, adding a buffer solution required by latex reagent labeling reaction into the latex solution after finishing the convergence polymerization reaction to perform the cross flow filtration. The method startsfrom the latex particle aspect, directly makes improvement on quadratic convergence polymerization through a simple amplifiable process, does not involves in improvement of antibody affinity, does not increase raw material cost, has wide range of application, effectively reduces difficulty of wide linear high antigen excess project development, and improves the latex immune turbidity method antigen excess and the linearity range.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis, and in particular relates to a method for improving the antigen excess and linear range of latex immunoturbidimetric method. Background technique [0002] In the field of in vitro diagnosis, antibodies or antigens are usually labeled on the surface of functionalized latex microspheres in latex immunoturbidimetric kits, and react with the tested antigen or antibody in a certain buffer solution to form antigen-antibody latex microsphere complexes, bringing Absorbance signal value changes. A standard curve of signal value and concentration is drawn using a standard product of known concentration. When measuring a sample of unknown concentration, the concentration of the analyte is calculated by the automatic analysis instrument based on the comparison between the signal value obtained from the sample and the standard curve. [0003] Antigen excess (safety zone) and linear range are one o...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor王钊王金凤蒋欣
OwnerBYRON DIAGNOSTICS SHANGHAI