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Preparation and application methods of blended ion exchange membrane

An ion exchange membrane and application method technology, applied in separation methods, chemical instruments and methods, membrane technology, etc., can solve the problems of reducing membrane flux, difficult to control the pH of the membrane chamber, and reducing the separation effect.

Active Publication Date: 2018-04-13
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the pore size is too small, the membrane flux will be reduced, and even protein molecules will be intercepted, thereby reducing the separation effect; if the pore size is too large, it will be difficult to control the pH of the membrane chamber and achieve the separation effect

Method used

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  • Preparation and application methods of blended ion exchange membrane

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The preparation and application method of a blended ion-exchange membrane in this embodiment relates to a blended ion-exchange membrane for separating myosin and bovine serum albumin and a separation device thereof, including the following steps: using N-methylpyrrolidone as a Solvent, prepare a casting solution containing 10% sulfonated polyaryl ether ketone and 15% polysulfone, and use a 250 μm scraper to prepare a blended membrane on a non-woven fabric to obtain an ion exchange membrane with a membrane pore size of 20 nm.

[0016] The separation device is divided into four membrane chambers by a composite membrane. Using 20mM tris(hydroxymethyl)aminomethane as a buffer solution, the pH values ​​of the four membrane chambers are controlled by acid-base automatic titration to be 4, 4.8, 7.0, and 9.5, respectively. The electrode solution of the anode is a phosphoric acid solution with a concentration of 0.033M, the electrode solution of the cathode is a sodium hydroxide ...

Embodiment 2

[0018] The concentration of the anode electrode solution of the above isoelectric focusing membrane system was changed to 0.033M phosphoric acid solution, the concentration of the cathode electrode solution was 0.1M sodium hydroxide solution, the buffer solution concentration was 50mM, and the current during the experiment was controlled at 80mA. , the measured myosin flux was 2.47 mg / LMh, and the purity in chamber 3 was 98.9%.

Embodiment 3

[0020] The concentration of the anode electrode solution of the above isoelectric focusing membrane system was changed to 0.1M phosphoric acid solution, the concentration of the cathode electrode solution was 0.2M sodium hydroxide solution, the buffer solution concentration was 35mM, and the current during the experiment was controlled at 80mA. , the measured myosin flux was 4.79 mg / LMh, and the purity in chamber 3 was close to 100%.

[0021] H 3 PO 4 (M)

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Abstract

The invention discloses a preparation method of a blended ion exchange membrane and an application method for protein separation. The preparation method comprises the following steps: taking N-methylpyrrolidone as a solvent, and taking sulfonated polyaryletherketone and polysulfone as film materials for preparing a casting solution, taking an aqueous solution of isopropyl alcohol as a coagulationbath, and using a scraper to prepare the blended membrane on a nonwoven fabric. The blended ion exchange membrane is applied to an isoelectric focusing membrane electrophoresis system, a device is divided into multiple membrane chambers by the ion exchange membrane, tris(hydroxymethyl)aminomethane is taken as a buffer solution, the pH value of each membrane chambers is controlled at a certain gradient by acid-base automatic titration, and different proteins are separated into different membrane chambers by using difference of the isoelectric point of different proteins and biomolecules under electric field conditions. The blended ion exchange membrane of the present invention can achieve product purity of up to 100%.

Description

technical field [0001] The invention belongs to the technical field of membrane separation, and in particular relates to a preparation and application method of a blended ion exchange membrane. Background technique [0002] Isoelectric focusing electrophoresis technology is a high-efficiency protein separation technology. Its principle is to use ampholyte to create a pH gradient in the gel, and to use the characteristics of amphoteric dissociation and isoelectric point of proteins to separate and analyze proteins. The protein isoelectric focusing electrophoresis technology has the advantages of high separation efficiency and simultaneous separation and enrichment. [0003] The isoelectric focusing thin film electrophoresis technology uses an ion exchange membrane to divide the continuous liquid phase into several membrane chambers, and forms a gradient by controlling the pH of the membrane chambers, so as to achieve the separation effect under the action of an electric field...

Claims

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Application Information

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IPC IPC(8): B01D57/02B01D67/00B01D71/76B01D69/12
CPCB01D57/02B01D67/0013B01D69/125B01D71/76
Inventor 周政忠陈莲花郑涛袁浩然
Owner CHANGZHOU UNIV
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