Step-by-step lignocellulose saccharifying fermentation method with polyvinyl glycol-water as medium

A technology of lignocellulose and polyethylene glycol, applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effect of reducing energy consumption of ethanol distillation, reducing equipment investment, and reducing total cost

Active Publication Date: 2018-04-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem of heat transfer, mass transfer and the suppression of ethanol fermentation caused by excessive toxic compounds in the enzymatic hydrolysis process of lignocellulose, and provides a step-by-step saccharification and fermentation of lignocellulose with polyethylene glycol-water as the medium Law

Method used

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  • Step-by-step lignocellulose saccharifying fermentation method with polyvinyl glycol-water as medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The fast-growing poplar pretreated by the steam explosion method, polyethylene glycol (PEG-1000), and deionized water are mixed according to a solid-to-liquid ratio of 10%, and the mass ratio of polyethylene glycol to deionized water is 20%, and then cellulase 15FPU is added / g substrate, under the conditions of pH 4.85 and temperature 50°C, carry out preliminary enzymatic hydrolysis for 24 hours, and conduct solid-liquid separation of the obtained enzymatic hydrolysis slurry, and the glucose concentration in the obtained hydrolyzate is 40g / L; The solid-liquid ratio is 10%, and the fast-growing poplar and cellulase 15FPU / g substrate pretreated by steam explosion method are added to carry out enzymatic hydrolysis for 24 hours again, and the obtained enzymatic hydrolysis slurry is subjected to solid-liquid separation again, and the glucose concentration of the obtained hydrolyzate is 70g / L; Add Saccharomyces cerevisiae OD to the obtained hydrolyzate 600 The temperature i...

Embodiment 2

[0027] The fast-growing poplar pretreated by the steam explosion method, polyethylene glycol (PEG-200), and deionized water are mixed according to a solid-to-liquid ratio of 10%, and the mass ratio of polyethylene glycol to deionized water is 20%, and then cellulase 15FPU is added / g substrate, under the conditions of pH 4.85 and temperature 50°C, carry out preliminary enzymatic hydrolysis for 24 hours, and conduct solid-liquid separation of the obtained enzymatic hydrolysis slurry, and the glucose concentration in the obtained hydrolyzate is 41g / L; Solid-to-liquid ratio 10% added fast-growing poplar pretreated by steam explosion method, cellulase 15FPU / g substrate and carried out enzymatic hydrolysis again for 24 hours, and the enzymatic hydrolysis slurry obtained was subjected to solid-liquid separation again, and the glucose concentration of the obtained hydrolyzate was 72g / L; Saccharomyces cerevisiae OD was added to the obtained hydrolyzate 600 The temperature is 2.0, and ...

Embodiment 3

[0029] The straw pretreated by steam explosion method, polyethylene glycol (PEG-2000), and deionized water are mixed according to the solid-to-liquid ratio of 10%, the mass ratio of polyethylene glycol to deionized water is 20%, and then cellulase 15FPU / g substrate, under the conditions of pH 4.85 and temperature 50°C, carry out preliminary enzymatic hydrolysis for 24 hours, and carry out solid-liquid separation of the obtained enzymatic hydrolysis slurry, and the glucose concentration in the obtained hydrolyzate is 45g / L; The liquid ratio is 10%, and the fast-growing poplar and cellulase 15FPU / g substrate pretreated by steam explosion method are added to carry out enzymatic hydrolysis for 24 hours again, and the obtained enzymatic hydrolysis slurry is subjected to solid-liquid separation again, and the glucose concentration of the obtained hydrolyzate is 86g / L; Add Saccharomyces cerevisiae OD to the obtained hydrolyzate 600 The temperature is 2.0, and the temperature is 33°...

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Abstract

The invention discloses a step-by-step lignocellulose saccharifying fermentation method with polyvinyl glycol-water as a medium. Pretreated lignocelluloses is used as a substrate, a polyvinyl glycol/water mixture is used as a fermentation medium, the strategy that the substrate and cellulase are supplemented in batches and enzymatic hydrolysate solid residues are removed step by step before fermentation is adopted, and the concentration of toxic inhibitors is reduced while the solid content is increased, so that high-concentration ethanol is obtained. The method has the advantages as follows:material supplement in batches contributes to heat transfer and mass transfer in the enzymolysis process, and a hydrosylate of high-concentration reducing sugar can be obtained; solid residues produced through enzymolysis can be used as an adsorbent of fermentation toxic inhibitors, fermentation toxic substances are effectively removed through centrifugal separation, the enzymatic hydrolysate canbe directly used for fermentation without detoxification treatment, and polyvinyl glycol can effectively relieve inhabitation of ethanol fermentation by residual toxic compounds in the hydrolysate; the hydrosylate of high-concentration reducing sugar can improve the concentration of ethanol produced through fermentation later, so that the production cost of cellulosic ethanol is reduced.

Description

technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis of lignocellulose, and in particular relates to a stepwise saccharification and fermentation method of lignocellulose using polyethylene glycol-water as a medium. Background technique [0002] With the depletion of fossil energy and the aggravation of environmental pollution, the development and utilization of renewable clean energy fuel ethanol has received widespread attention. Traditional ethanol fermentation uses sugar or starch as raw materials, both of which are the main sources of food. The production of fuel ethanol from grains has already constituted a threat to world food security, and it is imperative to find other raw materials to replace grains. Lignocellulose is the most abundant and cheap renewable resource in nature, and its main component, cellulose hemicellulose, is a potential raw material for the production of fuel ethanol. The use of lignocellulose to produce fuel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCC12P7/10C12P19/14C12P2201/00C12P2203/00Y02E50/10
Inventor 张宗超刘秀梅毛燎原
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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