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Method for separating and preparing gelsemium alkaloid from gelsemium elegans benth by virtue of ion exchange method

A technology of Gelkins alkaloids and ion exchange method, which is applied in the field of preparation of Gelkins alkaloids of medicinal plants, can solve the problems of large extraction liquid volume and long time consumption, and achieve the effect of simple process

Active Publication Date: 2018-04-20
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid water immersion extraction method takes a long time and the volume of the extraction solution is large

Method used

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  • Method for separating and preparing gelsemium alkaloid from gelsemium elegans benth by virtue of ion exchange method
  • Method for separating and preparing gelsemium alkaloid from gelsemium elegans benth by virtue of ion exchange method

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The herb was dried at 60°C, crushed through a 60-mesh sieve, added with 95% ethanol and heated under reflux for extraction, the extraction time was 1-3 h, and the filtrate was collected; the extraction was repeated 1-3 times, the combined filtrate was concentrated under reduced pressure, and the ethanol was recovered to No mellow smell, a crude extract is obtained. Add 5% hydrochloric acid to the obtained crude extract to adjust the pH to 3, filter; add hydrochloric acid solution (pH=2) to dissolve the precipitate, filter, operate 1-2 times, combine the filtrates, and prepare a concentration of 1.0 g crude drug / mL of the sample solution.

[0034] At room temperature, the sample solution with a concentration of 1.0 g crude drug / mL was passed through the chromatographic column equipped with pretreated 732 ion exchange resin (Shanghai Yuanye Biotechnology) at a flow rate of 2 BV / h for exchange adsorption, and 6 BV distilled water , 6 BV volume concentration 80% ethanol an...

Embodiment 2

[0036] The herb was dried at 60°C, crushed through a 60-mesh sieve, added with 95% ethanol and heated under reflux for extraction, the extraction time was 1-3 h, and the filtrate was collected; the extraction was repeated 1-3 times, the combined filtrate was concentrated under reduced pressure, and the ethanol was recovered to No mellow smell, a crude extract is obtained. Add 5% hydrochloric acid to the obtained crude extract to adjust the pH to 3, filter; add hydrochloric acid solution (pH=2) to dissolve the precipitate, filter, operate 1-2 times, combine the filtrates, and prepare a concentration of 1.0 g crude drug / mL of the sample solution.

[0037] At room temperature, the sample solution with a concentration of 1.0 g crude drug / mL was passed through the chromatographic column equipped with pretreated 732 ion exchange resin (Shanghai Yuanye Biotechnology) at a flow rate of 3 BV / h for exchange adsorption, and 6 BV distilled water , 6 BV volume concentration 80% ethanol an...

Embodiment 3

[0039]The herb was dried at 60°C, crushed through a 60-mesh sieve, added with 95% ethanol and heated under reflux for extraction, the extraction time was 1-3 h, and the filtrate was collected; the extraction was repeated 1-3 times, the combined filtrate was concentrated under reduced pressure, and the ethanol was recovered to No mellow smell, a crude extract is obtained. Add 5% hydrochloric acid to the obtained crude extract to adjust the pH to 3, filter; add hydrochloric acid solution (pH=2) to dissolve the precipitate, filter, operate 1-2 times, combine the filtrates, and prepare a concentration of 1.0 g crude drug / mL of the sample solution.

[0040] At room temperature, the sample solution with a concentration of 1.0 g crude drug / mL was passed through the chromatographic column equipped with pretreated 732 ion exchange resin (Shanghai Yuanye Biotechnology) at a flow rate of 5 BV / h for exchange adsorption, and 6 BV distilled water , 6 BV volume concentration 80% ethanol and...

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Abstract

The invention discloses a method for separating and preparing gelsemium alkaloid from gelsemium elegans benth by virtue of an ion exchange method. The method comprises the following steps: 1) heatingand conducting reflux extraction on a medicinal material, namely the separating and preparing gelsemium alkaloid from gelsemium elegans benth by virtue of ion exchange method, so that a coarse extracting solution is obtained; adding hydrochloric acid to the coarse extracting solution and regulating pH value to 2-5, and conducting filtering; dissolving a precipitate by virtue of a hydrochloric acidwater solution which is 2-5 in pH value, implementing filtering, and combining filtrates so as to prepare a loading solution which is 0.75-1.5g crude drug / mL in concentration; processing the loadingsolution by virtue of a pre-processed strong acid ion exchange resin column, wherein the flow rate of the loading solution is 1-5BV / h; after conducting exchange, rinsing the resin column to be neutral, removing impurities by virtue of ethanol which is 80% in volume concentration, and eluting the resin column by virtue of ammonium hydroxide which is 50% in volume concentration and ethanol which is90% in volume concentration; and collecting eluents, implementing decompressing and recovering the ethanol, and conducting drying, so that a gelsemium alkaloid extract is obtained. With the application of the method provided by the invention, the content of koumine is improved from 4.6% of the coarse extracting solution to be greater than 20% of the coarse extracting solution, so that a foundationis laid for further separation and preparation of the high-purity koumine.

Description

technical field [0001] The invention relates to the technical field of preparation of alkaloids of medicinal plants, in particular to a method for preparing kinesin alkaloids extracts from kinesin plants. Background technique [0002] hook kiss ( Gelsemium elegans Benth.) is an evergreen woody vine belonging to the genus of Strychtenaceae. The whole plant is used as medicine, which has the effects of "reducing swelling and pain, dispelling wind and blood stasis, attacking poison and killing insects". The main active ingredients of Gelkins are indole alkaloids, which have pharmacological effects such as anti-inflammatory and analgesic, anti-tumor, anti-psoriasis, and promoting the growth of poultry and livestock. Gelkins alkaloids include nearly 100 kinds of monomers such as keloids, jia, yin, and jiu. In recent years, a series of studies have found that kelezin has significant analgesic and anti-anxiety effects, and has a good prospect for industrial medicinal use. The p...

Claims

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Application Information

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IPC IPC(8): A61K36/56C07D491/22A61P29/00A61P35/00A61P17/06A61P43/00A23L33/105A23K20/116
CPCA23V2002/00A61K36/56A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A23K20/116A23L33/105C07D491/22A23V2200/30A23V2250/21
Inventor 俞昌喜石冬梅许盈苏燕评何灿强
Owner FUJIAN MEDICAL UNIV
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