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Manufacturing method for plant heavy metal enrichment accelerant based on pseudomonas syringae

A technology of Pseudomonas and heavy metals, applied in the biological field, can solve the problems of low biomass, slow growth, low dry matter accumulation, etc.

Inactive Publication Date: 2018-04-20
EASTERN GANSU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the importance and broad application prospects of hyperaccumulator plants are gradually being recognized by more people, their role in remediating soil pollution in large-scale production practices is still limited.
The main reason is that hyperaccumulator plants are adaptive mutants that have been induced and domesticated for a long time under heavy metal stress. They often grow slowly, have low biomass, and accumulate less dry matter. The amount of heavy metals that can be absorbed per unit time is determined by Restricted to a low level, which directly limits the repair effect

Method used

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  • Manufacturing method for plant heavy metal enrichment accelerant based on pseudomonas syringae
  • Manufacturing method for plant heavy metal enrichment accelerant based on pseudomonas syringae
  • Manufacturing method for plant heavy metal enrichment accelerant based on pseudomonas syringae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1, preparation of a heavy metal enrichment accelerator based on Pseudomonas syringae: (1) Preparation of Pseudomonas syringae, Pst The medium of DC3000 strain is all low-salt LB medium (recipe: each liter of medium contains: peptone 10g; yeast extract powder 5g; sodium chloride 5g). The DC3000 strain stored at -80 °C was taken out of the ultra-low temperature refrigerator, streaked onto the low-salt LB solid medium containing an appropriate amount of antibiotics, and cultured at 28 °C for 36-48 h. A single colony was picked, inoculated into a 10 ml seed tube, and cultured on a constant temperature shaker at 28 °C and 120 rpm for about 10 h. The fresh culture was inoculated into low-salt LB liquid medium containing appropriate amount of antibiotics according to the ratio of 1:100, and continued to grow overnight on a constant temperature shaker at 28 °C and 120 rpm until OD600≈0.8. Transfer the final product to a centrifuge tube, centrifuge at 1500 g for 20 mi...

Embodiment 2

[0037] Example 2, soil for testing: collected from uncontaminated wheat field surface soil (0-20cm) in the suburbs of Qingyang City, Gansu Province: the organic matter content is 10.58 g·kg −1 , total nitrogen 0.82 g·kg −1 , Alkaline hydrolyzed nitrogen 52.45 mg·kg −1 , total phosphorus 0.659g·kg −1 , available phosphorus 11.24 mg·kg −1 , available potassium 152.88 mg·kg −1 , total cadmium: 0.039 mg.kg -1 . With reference to my country's "Soil Environmental Quality Standards, 1995", two cadmium levels were set up in this embodiment, Cdl (1 mg / kg soil) and Cd3 (3 mg / kg soil), to simulate light and moderate cadmium pollution in farmland soil respectively. And by artificially adding 3CdSO 4 .8H 2 O solution is realized. 20 kg of well-mixed soil at each cadmium level was packed into plastic turnover boxes respectively, and the size of the turnover box was 42 cm (length) X 32 cm (width) X 16 cm (height). In order to stabilize various adsorption balance characteristics of th...

Embodiment 3

[0040] Embodiment 3, test soil is the same as embodiment 2.

[0041] Test plant: Tobacco (variety name: Yunyan-87), tobacco seeds were sprayed with 3% H before sowing 2 o 2 Soak for 10 minutes, then rinse 3 times with tap water, and grow seedlings in the greenhouse. After 1 month, transplant the seedlings into the turnover box (8 plants / box), water every day to keep the soil moisture suitable, and there is no soil moisture during the whole test period. Add any fertilizer. Randomly grouped, 1 week before the harvest, spray water (as a control) or the plant heavy metal enrichment accelerator (spray) prepared in Example 1 respectively on the leaf surface, and then cover the agricultural film to keep the relative humidity of the environment RH≥ 80% until harvest.

[0042] The plants were transplanted to the turnover box and harvested after 2 months. The residual amount of cadmium in soil was determined by flame atomic absorption spectrometry [flame atomic absorption spectrosco...

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Abstract

The invention relates to a manufacturing method for a plant heavy metal enrichment accelerant based on pseudomonas syringae. The method is characterized in that the plant heavy metal enrichment accelerant comprises the pseudomonas syringae, potassium bisulfate and a surface active agent. A preliminary test is conducted on tomatoes and tobaccos. Results show that (1) the accelerant can remarkably promote the enrichment effect of plants on Cd, Pb and Cu, and the enrichment capacity of the tested plants can be improved by at least two times; and (2) the accelerant can remarkably promote improvement of expression of heavy metal transfer related protein genes, including LeNRAMP1, LeNRAMP3 and LeIRT1, in the tomatoes. In conclusion, the plant heavy metal enrichment accelerant based on the pseudomonas syringae is a new efficeint agent for promoting heavy metal enrichment of the plants and has broad application prospects.

Description

technical field [0001] The invention relates to a preparation method of a plant heavy metal enrichment accelerator based on Pseudomonas syringae, and belongs to the field of biotechnology. Background technique [0002] Heavy metal pollution is one of the major environmental hazards in the world, and may enter the human food chain through crops, seriously threatening human life and health. Phytoremediation is an environmentally friendly and cheap method for the treatment of heavy metal pollution, but the effect of remediation is not satisfactory, and it is difficult to promote and apply it. The enrichment effect is the key factor restricting the phytoremediation of heavy metal pollution. Although the importance and broad application prospects of hyperaccumulator plants are gradually being recognized by more people, their role in remediating soil pollution in large-scale production practices is still limited. The main reason is that hyperaccumulator plants are adaptive mutan...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/00C12N1/20C12R1/38
CPCB09C1/10B09C1/105C12N1/20
Inventor 欧晓彬燕晓娟张文杰王东
Owner EASTERN GANSU UNIVERSITY
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