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Anti-cyclic citrullinated peptide antibody detection kit

A technology of anti-cyclic citrullinated peptide and detection kit, which is applied in the fields of medicine and biochemistry, can solve the problems of difficulty in meeting clinical detection requirements, inconvenient use, and the inability of the kit to meet the wide linear range and high sensitivity at the same time, and achieve stable Improved performance, wide linear range, and improved linear range

Active Publication Date: 2018-04-20
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are kits for the determination of anti-CCP antibody concentration by latex-enhanced immunoturbidimetric method on the market, but these kits cannot meet the requirements of wide linear range and high sensitivity at the same time, so it is necessary to dilute the sample to measure high-concentration anti-CCP antibody. Inconvenient to use
The existing latex-enhanced immunoturbidimetric method, such as the detection sensitivity of the kit disclosed in patent CN102507918B and CN 104198725B, is greater than or equal to 1.25AU / ml, and the linear measurement range is below 200AU / ml, which are difficult to meet the clinical detection needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 kit preparation 1

[0038] Reagent R1: pH7.4

[0039] Tris 50mM

[0040] Triton X-100 1%

[0041] Polyethylene glycol 6000 4g / l

[0042] Sodium chloride 10g / l

[0043] The preparation process of reagent R2 is as follows:

[0044] (1) Dilute polystyrene particles with carboxyl functional groups on the surface and 0.8 μm in diameter with MES buffer to a final concentration of 0.5-3% (wt), add 50-90mM EDAC, mix well, and shake at room temperature for 30 -80 minutes;

[0045] (2) Centrifuge the mixture obtained in step (1), discard the supernatant, wash twice with MES buffer, and finally resuspend the latex particles in MES buffer to a final concentration of 2%, and disperse them by ultrasonic to obtain a particle dispersion ;

[0046] (3) Dilute the anti-cyclic citrullinated peptide antigen with MES buffer, mix it with the microparticle dispersion in step (2) in equal volume, and react at room temperature for 2-4 hours;

[0047] (4) After centrifuging ...

Embodiment 2

[0061] Embodiment 2 kit preparation 2

[0062] Reagent R1: pH7.4

[0063] Tris 50mM

[0064] Triton X-100 1%

[0065] Polyethylene glycol 6000 4g / l

[0066] Sodium chloride 10g / l

[0067] The preparation process of reagent R2 is as follows:

[0068] (1) Dilute polystyrene particles with carboxyl functional groups on the surface and 0.8 μm in diameter with MES buffer to a final concentration of 0.5-3% (wt), add 50-90mM EDAC, mix well, and shake at room temperature for 30 -80 minutes;

[0069] (2) Centrifuge the mixture obtained in step (1), discard the supernatant, wash twice with MES buffer, and finally resuspend the latex particles in MES buffer to a final concentration of 2%, and disperse them by ultrasonic to obtain a particle dispersion ;

[0070] (3) Dilute the anti-cyclic citrullinated peptide antigen with MES buffer, mix it with the microparticle dispersion in step (2) in equal volume, and react at room temperature for 2-4 hours;

[0071] (4) After centrifuging ...

Embodiment 3

[0087] Embodiment 3 kit preparation 3

[0088] Reagent R1: pH7.4

[0089] Tris 50mM

[0090] Triton X-100 1%

[0091] Polyethylene glycol 6000 4g / l

[0092] Sodium chloride 10g / l

[0093] The preparation process of reagent R2 is as follows:

[0094] (1) Dilute polystyrene particles with carboxyl functional groups on the surface and 0.8 μm in diameter with MES buffer to a final concentration of 0.5-3% (wt), add 50-90mM EDAC, mix well, and shake at room temperature for 30 -80 minutes;

[0095] (2) Centrifuge the mixture obtained in step (1), discard the supernatant, wash twice with MES buffer, and finally resuspend the latex particles in MES buffer to a final concentration of 2%, and disperse them by ultrasonic to obtain a particle dispersion ;

[0096] (3) Dilute the anti-cyclic citrullinated peptide antigen with MES buffer, mix it with the microparticle dispersion in step (2) in equal volume, and react at room temperature for 2-4 hours;

[0097] (4) After centrifuging ...

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Abstract

The invention belongs to the technical fields of medical science and biochemistry, and concretely relates to an anti-cyclic citrullin peptide antibody detection kit. The kit comprises a reagent R1 anda reagent R2, and the reagent R1 includes a biological buffer agent, a coagulating accelerator, a surfactant, an osmotic pressure regulator and water; and the reagent R2 includes latex particles coated with the anti-cyclic citrullin peptide antigen, the biological buffer agent, a blocking agent, a preservative and water. Compared with traditional anti-cyclic citrullinated peptide antibody detection kits, the anti-cyclic citrullinated peptide antibody detection kit which uses latex-enhanced immunoturbidimetry to perform detection in the invention has the advantages of rapidity, sensitivity, good accuracy, high specificity and good stability.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring anti-cyclic citrullinated peptide antibodies. Background technique [0002] Rheumatoid arthritis (RA) is a common multi-systemic inflammatory autoimmune disease mainly involving the surrounding joints. Irreversible bone and joint damage can occur within two years of onset and can cause a variety of concurrent complications. Diseases, such as nervous system diseases, pericarditis, lymphadenitis, etc. Early diagnosis and judgment of the development trend of the disease will help clinicians to control the disease with drugs that improve the course of the disease and effectively reduce the occurrence of joint erosion. At present, the diagnosis of RA is still mainly based on the classification diagnosis revised by the American Rheumatology Association in 1987, such as clinical manifestations, X-rays, and rheumatoid factor (RF). Patients who meet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6854G01N33/6893G01N2800/102
Inventor 吴正伟章晓庆
Owner 浙江夸克生物科技有限公司
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