Application of mir-146a-5p to treating visceral hypersensitivity of irritable bowel syndrome

A technology of 1. mir-146a-5p, mir-146a-5pagomir, applied in the medical field, can solve the problems of intestinal permeability serotonin signaling pathway and abnormal visceral or somatic hypersensitivity, etc. effect of visceral hypersensitivity

Active Publication Date: 2018-04-24
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that microRNA is abnormally expressed in patients with irritable bowel syndrome (IBS), which leads to abnormalities in intestinal permeability, serotonin sign

Method used

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  • Application of mir-146a-5p to treating visceral hypersensitivity of irritable bowel syndrome
  • Application of mir-146a-5p to treating visceral hypersensitivity of irritable bowel syndrome
  • Application of mir-146a-5p to treating visceral hypersensitivity of irritable bowel syndrome

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Experimental program
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Effect test

Embodiment approach

[0028] Visceral hypersensitivity refers to the increased sensitivity and responsiveness of visceral tissue to various mechanical and chemical stimuli, including visceral hyperalgesia and allodynia, and the volume and pressure thresholds of the colorectum are significantly reduced during dilation stimulation. At present, the mechanism of visceral hypersensitivity in IBS has not been fully elucidated, which may include increased sensitivity of visceral receptors, abnormal sensory afferents, and increased excitability of central visceral sensory neurons. Low-grade inflammation is also involved in the formation of visceral hypersensitivity in IBS.

[0029] Agomirs are specially labeled and chemically modified double-stranded small RNAs that modulate the biological functions of target genes by mimicking endogenous miRNAs.

[0030] In order to make the above-mentioned and other objects, features and advantages of the present invention more obvious and easy to understand, the preferr...

Embodiment 1

[0031] Example 1 Detection of serum mir-146a-5p expression in patients with irritable bowel syndrome

[0032]The included research subjects included 5 patients with irritable bowel syndrome (IBS) who were treated in the Department of Gastroenterology, Qilu Hospital of Shandong University (with high visceral hypersensitivity), and 5 healthy volunteers were included in the healthy control group. The diagnostic criteria of diarrhea-predominant irritable bowel syndrome refer to Rome III diagnostic criteria. Exclusion criteria were: patients with other diseases causing diarrhea (tumor, thyroid disease, celiac disease, etc.); patients with a history of abdominal surgery or alarm symptoms (anemia, gastrointestinal bleeding, significant weight loss, abdominal mass); patients with coagulation Abnormal function or serious organic disease affecting heart, liver, kidney function; pregnant or breastfeeding. 3ml of venous blood from 10 subjects was drawn into a coagulation tube, left stand...

Embodiment 2

[0034] Example 2 Establishment of a mouse model of irritable bowel syndrome and agomir intervention

[0035] The mice were fasted for 24 hours, and the mice were anesthetized by intraperitoneal injection of 1% pentobarbital (0.2ml / mice). The anal tube was inserted into the anus of the mouse, and TNBS (80mg / kg) was injected into the anus about 5cm away from the anus. A model was established, and the blank control group was given an equal volume of normal saline for enema. After the enema, the mice were inverted for about 30 minutes to prevent the liquid from flowing out. The Agomir intervention method was to start hsa-mir-146a-5p agomir (3nmol / a) enema seven days after TNBS modeling, and enema every three days. The disease model control group and blank control group were given equal volume of normal saline enema. On the 28th day, colonic dilatation test was performed to evaluate visceral hypersensitivity, and then the mice were sacrificed to collect colonic tissue for follow-up...

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Abstract

The invention discloses application of mir-146a-5p to treating visceral hypersensitivity of irritable bowel syndrome. The mir-146a-5p is low in expression in peripheral serum of patients with visceralhypersensitivity of irritable bowel syndrome and intestinal mucosa of disease model mice with irritable bowel syndrome; meanwhile, after the disease model mice of irritable bowel syndrome receive intraperitoneal injection of hsa-mir-146a-5p agomir to achieve high expression of mir-146a-5p, the visceral sensitivity of the disease model mice is significantly reduced, so that the effectiveness of mir-146a-5p on reducing visceral hypersensitivity of the irritable bowel syndrome can be proved, and an effective way for treating visceral hypersensitivity of irritable bowel syndrome can be provided.

Description

technical field [0001] The invention belongs to the field of medical technology, in particular to the application of non-coding small RNA gene mir-146a-5p, in particular to the application of mir-146a-5p in the preparation of a medicine for treating irritable bowel syndrome patients with high visceral sensitivity. Background technique [0002] Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain or discomfort and changes in bowel habits without morphological and biochemical changes. The pathophysiological mechanism of IBS has not yet been explored. One of the most common types of digestive system diseases, it has become an important factor affecting the quality of life of patients and increasing the social and economic burden. About 10%-20% of adults and adolescents worldwide have symptoms of IBS, and the majority are women. [0003] Several studies have shown that about 30% to 40% of IBS patients have symptoms of hypersen...

Claims

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Application Information

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IPC IPC(8): A61K31/7105A61P1/00
CPCA61K31/7105
Inventor 李延青李竹青左秀丽李理想
Owner SHANDONG UNIV QILU HOSPITAL
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