Fermentation feeding optimizing control system and method thereof

Abstract

The invention discloses a fermentation feeding optimizing control system and a method thereof. The system comprises a fermenting tank, a feeding bottle, a glucose electrode used for monitoring the concentration of sugar in the fermenting tank in real time, an optical density electrode used for monitoring the OD value of thalli in the fermenting tank in real time, an external feeding pump used forpumping a feeding medium, and an external computer control system connected with the glucose electrode, the optical density electrode and the feeding pump, and the running speed of the feeding pump iscontrolled according to the concentration of the sugar, the OD value of the thalli and a preset specific growth rate in order to supplement the feeding medium to the fermenting tank from the feedingbottle in a growth rate control feeding mode and a continuous fed-batch form. The system and the method can improve the automation degree of fermentation feeding, and facilitates the improvement of the fermenting density of the thalli in order to improve the expression of target proteins.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a system and method for optimizing and controlling fermentation feed. Background technique [0002] Fed-batch fermentation, also known as semi-continuous fermentation, refers to the method of adding fresh medium or certain nutrients intermittently or continuously during batch fermentation. At present, the scope of application is very wide, almost throughout the entire fermentation industry. The theoretical research on fed-batch fermentation was almost blank before the 1970s. In the early industrial production, the method of feeding was very simple, and the methods used were limited to intermittent feeding and constant feeding to control fermentation. It is also based on experience. Until 1973, when Japanese scholar Yoshida et al proposed the term "fed-batch fermentation" and theoretically established the first mathematical model, the research on fed-batch fermentation ente...

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Problems solved by technology

[0004] The deficiencies of the prior art are as follows: (1) it is necessary to continuously measure the OD in the fermentation process and the residual sugar to carry out feedback adjustment feeding speed; (2) the manual operation requirement is high and the time is long in the fermentation process; (3) the fermentation process The probability of bacterial infection increases during the process; (4) In the traditional fermentation broth production process, the feeding system is a "semi-starved" feeding process, or all the nutrients in the medium are added at one time in the early stage of fermentation and allowed to be consumed gradually; Manual and intermittent feeding in the middle and late stage of fermentation increases the fluctuation of the overall ecological environment of the culture medium, and the loss of fermentation yield is large

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