A fermentation feed optimization control system and method

Abstract

The invention discloses a fermentation feeding optimization control system and method. The system includes a fermentation tank, a feeding bottle, a glucose electrode for real-time monitoring of the sugar concentration in the fermentation tank, and a glucose electrode for real-time monitoring of the sugar concentration in the fermentation tank. The optical density electrode of the bacterial cell OD, as well as an external feeding pump and an external computer control system for pumping the feeding medium, which are connected to the glucose electrode, optical density electrode and feeding pump, according to the sugar concentration, bacteria The body OD and the set specific growth rate control the running speed of the feeding pump, and the feeding medium is replenished from the feeding bottle to the fermentation tank in the feeding mode of controlling the specific growth rate and in the form of continuous feeding. . The system and method of the present invention can improve the automation process of fermentation feeding and are beneficial to increasing the fermentation density of bacterial cells and improving the expression of target proteins.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a system and method for optimizing and controlling fermentation feed. Background technique [0002] Fed-batch fermentation, also known as semi-continuous fermentation, refers to the method of adding fresh medium or certain nutrients intermittently or continuously during batch fermentation. At present, the scope of application is very wide, almost throughout the entire fermentation industry. The theoretical research on fed-batch fermentation was almost blank before the 1970s. In the early industrial production, the method of feeding was very simple, and the methods used were limited to intermittent feeding and constant feeding to control fermentation. It is also based on experience. Until 1973, when Japanese scholar Yoshida et al proposed the term "fed-batch fermentation" and theoretically established the first mathematical model, the research on fed-batch fermentation ente...

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Problems solved by technology

[0004] The deficiencies of the prior art are as follows: (1) it is necessary to continuously measure the OD in the fermentation process and the residual sugar to carry out feedback adjustment feeding speed; (2) the manual operation requirement is high and the time is long in the fermentation process; (3) the fermentation process The probability of bacterial infection increases during the process; (4) In the traditional fermentation broth production process, the feeding system is a "semi-starved" feeding process, or all the nutrients in the medium are added at one time in the early stage of fermentation and allowed to be consumed gradually; Manual and intermittent feeding in the middle and late stage of fermentation increases the fluctuation of the overall ecological environment of the culture medium, and the loss of fermentation yield is large

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