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Preparation method of dopamine stabilized Pickering emulsion and application thereof on immobilized enzymes

A Pickering emulsion, dopamine technology, applied in biochemical equipment and methods, immobilized on/in organic carriers, enzymes, etc., can solve the problem of large resistance to enzyme catalysis mass transfer, complicated operation and production steps, and high preparation costs problems, to achieve good economic feasibility and practicability, good reusability, and low cost.

Inactive Publication Date: 2018-05-01
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation and production steps of this method are complicated, the preparation cost is high, and the enzyme-catalyzed mass transfer resistance at the oil-water interface is large, and the enzyme activity is not high.

Method used

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  • Preparation method of dopamine stabilized Pickering emulsion and application thereof on immobilized enzymes
  • Preparation method of dopamine stabilized Pickering emulsion and application thereof on immobilized enzymes
  • Preparation method of dopamine stabilized Pickering emulsion and application thereof on immobilized enzymes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 20 μL free enzyme solution and add 180 μL Tris buffer (10 mM, pH 8.5), mix well and add to the solution dispersed with 20 mg hydrophobically modified SiO 2 In 2mL of n-heptane containing nanoparticles, homogenize at high speed (15000rpm, 2min), then add 0.25mg / mL dopamine into the emulsion, stir homogeneously for 1min, and incubate in a shaker for 24h (180rpm, 28°C) to obtain A dopamine-stabilized Pickering emulsion-immobilized enzyme system.

[0035] The catalytic effects of free and immobilized enzymes were measured by the esterification of n-hexanol and n-hexanoic acid in water / n-heptane system. Will contain 400mmol L -1 N-hexanoic acid and 400mmol L -1 A solution of n-hexanol in 2 mL of n-heptane was added to the enzyme-coated Pickering emulsion. The enzyme-catalyzed reaction was carried out at 37°C in a water-bath shaker at 200 rpm for 1 hour, and 1 μL of the product was taken to analyze the results by gas chromatography, as shown in image 3 shown. The r...

Embodiment 2

[0037] Take 20 μL free enzyme solution and add 180 μL Tris buffer (10 mM, pH 8.5), mix well and add to the solution dispersed with 20 mg hydrophobically modified SiO 2 In 2mL of n-heptane containing nanoparticles, homogenize at high speed (20000rpm, 2min). Then 2mg / mL dopamine was added to the emulsion, stirred homogeneously for 1min, and incubated in a shaker for 24h (180rpm, 28°C) to obtain a dopamine-stabilized Pickering emulsion-immobilized enzyme system.

[0038] The catalytic effects of free and immobilized enzymes were measured by the esterification of n-hexanol and n-hexanoic acid in water / n-heptane system. Will contain 400mmol L -1 N-hexanoic acid and 400mmol L -1 A solution of n-hexanol in 2 mL of n-heptane was added to the enzyme-coated Pickering emulsion. The enzyme-catalyzed reaction was carried out at 37°C in a water-bath shaker at 200 rpm for 1 hour, and 1 μL of the product was taken to analyze the results by gas chromatography, as shown in image 3 shown. ...

Embodiment 3

[0040] Take 20 μL free enzyme solution and add 180 μL Tris buffer (10 mM, pH 8.5), mix well and add to the solution dispersed with 20 mg hydrophobically modified SiO 2 In 2mL of n-heptane containing nanoparticles, homogenize at high speed (20000rpm, 2min). Afterwards, 5 mg / mL dopamine was added to the emulsion, stirred homogeneously for 1 min, and incubated in a shaker for 24 h (180 rpm, 28°C) to obtain a dopamine-stabilized Pickering emulsion-immobilized enzyme system.

[0041] The catalytic effects of free and immobilized enzymes were measured by the esterification of n-hexanol and n-hexanoic acid in water / n-heptane system. Will contain 400mmol L -1 N-hexanoic acid and 400mmol L -1 A solution of n-hexanol in 2 mL of n-heptane was added to the enzyme-coated Pickering emulsion. The enzyme-catalyzed reaction was carried out at 37°C in a water-bath shaker at 200 rpm for 1 hour, and 1 μL of the product was taken to analyze the results by gas chromatography, as shown in image...

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Abstract

The invention relates to a preparation method of a dopamine stabilized Pickering emulsion and application thereof on immobilized enzymes. The preparation method is characterized by that hydrophobic modified SiO2 particles are used as a stabilizer, n-heptane is used as an oil phase, a Tris buffer is used as an aqueous phase, and a high speed homogenizer is used for stirring to obtain a SiO2 particle stabilized Pickering emulsion; dopamine is added into the SiO2 particle stabilized Pickering emulsion, and the obtained emulsion is incubated in a water bath shaker at room temperature to produce ablack Pickering emulsion stabilized by both SiO2 and polydopamine. Enzymes are immobilized in an emulsion aqueous phase to determine the stability and application performance of the Pickering emulsion. The prepared dopamine stabilized Pickering emulsion consists of SiO2 nanometer particles and a polymer coating layer, so that the stability is good, and coating of various enzymes can be realized, thereby making the immobilized enzymes have very good thermal stability, storage stability and reusability; and the Pickering emulsion is low in cost, and the preparation method is easy for operation,so that the Pickering emulsion has very good economic feasibility and practicality.

Description

technical field [0001] The invention relates to a preparation method of a dopamine-based stable water-in-oil Pickering emulsion and the application of an immobilized enzyme, belonging to the technical field of emulsifiers. Background technique [0002] Pickering emulsions are special emulsions stabilized by solid particles at the oil-water interface. The biggest difference between it and ordinary emulsions is that the system is stabilized by solid particles, while traditional emulsions are stabilized by various types of surfactants. The advantages of Pickering emulsion mainly include: low cost, less amount of emulsifier used; less toxicity, less environmental pollution; easy to form stable emulsion, less affected by external pH, temperature and system type. Therefore, Pickering emulsions are widely used in many systems such as drug sustained release, enzyme immobilization, and renewable energy. For the Pickering emulsion with stable solid particles, its stability depends o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/03C08L79/04C08K9/00C08K3/36C08G73/06C12N11/08
CPCC08G73/0672C08J3/03C08J2379/04C08K3/36C08K9/00C12N9/20C12N11/08C12Y301/01003
Inventor 齐崴黄仁亮屈彦宁苏荣欣
Owner TIANJIN UNIV
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