Extraction method of wormwood essential oil and its application in inhibiting plant pathogens
A technology of wormwood essential oil and plant pathogens, applied in the direction of plant/algae/fungus/moss components, applications, plant raw materials, etc., can solve the problems that the application is still blank, and achieve the effect of low price, wide source and high extraction rate
Active Publication Date: 2021-01-08
TOBACCO RES INST CHIN AGRI SCI ACAD
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Problems solved by technology
At present, the application of wormwood essential oil is mainly used for animal-derived pathogens such as Escherichia coli and Staphylococcus aureus, and its application in the inhibition of plant-derived pathogens is still blank
Method used
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Embodiment 1
[0019] Example 1 Supercritical CO 2 Extraction of wormwood essential oil
[0020] Weigh 70g wormwood powder sample, put into supercritical CO 2 Extraction device; turn on the water bath, make the extraction temperature reach 35°C, the extraction pressure 200bar (20MPa), CO 2 The flow rate is 20g / min, the temperature of the separation tank is 30°C, and the operation is 90min. After the extraction is completed, wash it out with absolute ethanol, centrifuge to remove waxy components, and anhydrous sodium sulfate to remove water. The filtrate was decompressed at 30°C, and the ethanol was recovered to obtain the dewaxed Artemisia argyi volatile oil.
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The invention relates to an extraction method of essential oil of artemisia argyi. The extraction method comprises the following steps: obtaining plants of artemisia argyi from saline land, drying plants of artemisia argyi at a temperature of 40 DEG C until the weight is constant, grinding and sieving the plants to obtain artemisia argyi powder; adding an artemisia argyi powder sample into a supercritical CO2 extraction apparatus, starting a water bath until the extraction temperature reaches 35 DEG C, carrying out extraction for 90 minutes under following conditions: extraction pressure: 20 MPa, CO2 flowing speed: 20 g / min, and temperature of separation kettle: 30 DEG C; after extraction, washing out the extract by anhydrous ethanol, carrying out centrifugation to remove wax, removing water by anhydrous sodium sulfate; and subjecting filtrate to reduced pressure distillation at a temperature of 30 DEG C to recover ethanol and obtain wax free volatile oil of artemisia argyi. The provided method can quickly and efficiently extract essential oil of artemisia argyi. The essential oil comprises many components. The aromatic components of essential oil of artemisia argyi can be well maintained. The essential oil of artemisia argyi has a good effect on inhibiting xanthomonas oryzae, ralstonia solanacearum, pathogenic bacteria that causes cucumber bacterial angular leaf spot, pathogenic bacteria that causes bacterial fruit blotch of cucurbit, potato rhizoctonia solani, soft-rot fungi of cabbage, and the like.
Description
technical field [0001] The invention belongs to the field of plant disease prevention and control, and in particular relates to a method for extracting wormwood essential oil and its application in inhibiting plant pathogenic bacteria. Background technique [0002] The contribution of pesticides to human beings is self-evident. In my country, through the use of pesticides, the economic loss caused by plant diseases can be reduced by about 30 billion yuan each year. However, pesticides are chemicals with high toxicity and high environmental release rate. , Substances with a wide range of effects, they also enter the environment while protecting crops. At the beginning of the development of organic chemical pesticides, people ignored the adverse effects of irrational use of chemical pesticides on humans and the environment, such as environmental pollution, human and animal poisoning, damage to natural enemies and harmless organisms, pesticide residues, harmful Bio-resistance a...
Claims
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IPC IPC(8): A61K36/282C11B9/02A01N65/12A01P1/00A01P3/00
CPCA01N65/00A01N65/12A61K36/282C11B9/025
Inventor 付秋娟荆常亮杜咏梅刘新民解胜男
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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