Novel environmental modifier for livestock housing as well as preparation method and application of novel environmental modifier

A technology of improving agent and environment, which is applied in the field of new-type livestock and poultry house environmental improver and its preparation field, can solve the problems of livestock and poultry toxicity, secondary pollution, health hazards of breeders, etc., and achieves purification of breeding environment and reduction of ammonia gas content. , the effect of non-toxic side effects

Inactive Publication Date: 2018-05-04
BEIJING DAWEIJIA BIOTECH SHARE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the process of modern large-scale livestock and poultry breeding, a large amount of dust, harmful gases and pathogenic bacteria will be produced in the pen, which will not only affect the quality of livestock and poultry, but also cause harm to the health of the breeders
Traditional ventilation methods cannot fundamentally solve ammonia pollution and cannot effectively inhibit pathogenic bacteria; although spraying chemical disinfectants can quickly eliminate ammonia and kill pathogenic bacteria, it is easy to cause secondary pollution, and it will also harm the livestock and poultry itself. cause certain toxicity

Method used

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  • Novel environmental modifier for livestock housing as well as preparation method and application of novel environmental modifier
  • Novel environmental modifier for livestock housing as well as preparation method and application of novel environmental modifier
  • Novel environmental modifier for livestock housing as well as preparation method and application of novel environmental modifier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Enterococcus faecium ( Enterococcus faecium ) WEI-9's first-level shake flask seed preparation:

[0043] Pick a loop of lawn from the freshly activated Enterococcus faecium WEI-9 slant (MRS medium) and inoculate it into a 500 mL Erlenmeyer flask containing 200 mL of WEI-9 seed medium. Inoculate 2 flasks in total and let stand at 37°C Cultivate for 12 h. After the cultivation, the pH of the culture solution and the number of viable bacteria were measured, and the purity of the bacterial solution was confirmed by microscopic examination.

[0044] The composition of Enterococcus faecium WEI-9 seed medium is as follows: peptone 18 g / L, glucose 12 g / L, sodium acetate trihydrate 2 g / L, dipotassium hydrogen phosphate trihydrate 0.8 g / L, magnesium sulfate heptahydrate 0.6 g / L, manganese sulfate monohydrate 0.02 g / L, Tween 80 1 g / L, pH 7.5.

[0045] Result analysis: After the above-mentioned culture of Enterococcus faecium WEI-9, the number of viable bacteria can reach 1...

Embodiment 2

[0046] Example 2: Lactobacillus plantarum ( Lactobacillus plantarum ) Preparation of WEI-12 first-level shake flask seeds:

[0047] The freshly activated Lactobacillus plantarum WEI-12 (MRS medium) slope was washed with 5 mL of sterile saline, and 2 mL was inoculated into a 500 mL Erlenmeyer flask containing 200 mL of WEI-12 seed medium for a total of 2 Incubate the bottle at 37°C for 24 h. After the cultivation, the pH of the culture solution and the number of viable bacteria were measured, and the purity of the bacterial solution was confirmed by microscopic examination.

[0048] The composition of Lactobacillus plantarum WEI-12 seed culture medium is as follows: yeast extract 16 g / L, whey powder 20 g / L, ammonium citrate 3 g / L, anhydrous sodium acetate 2 g / L, heptahydrate magnesium sulfate 0.3 g / L, Tween 80 1 g / L, pH 7.0.

[0049] Result analysis: After the above-mentioned cultivation of Lactobacillus plantarum WEI-12, the number of viable bacteria can reach 1.3×10 9 CFU / mL, c...

Embodiment 3

[0050] Example 3: Bacillus coagulans ( Bacillus coagulans ) Preparation of WEI-125 first-level shake flask seeds:

[0051] Pick a loop of lawn from the freshly activated Bacillus coagulans WEI-125 slant (MRS medium) and inoculate it into a 1 L Erlenmeyer flask containing 200 mL of WEI-125 seed medium. Inoculate 2 bottles in total, 30°C, 220 rpm Cultivate for 24 h. After the cultivation, the pH of the culture solution and the number of viable bacteria were measured, and the purity of the bacterial solution was confirmed by microscopic examination.

[0052] The composition of the Bacillus coagulans WEI-125 seed medium is as follows: peptone 10 g / L, yeast extract 10 g / L, glucose 20 g / L, pH 6.5.

[0053] Result analysis: After the above-mentioned cultivation of Bacillus coagulans WEI-125, the number of viable bacteria can reach 1.5×10 8 CFU / mL, the pH of the culture medium is 4.70, and the microscopic purity is 100%.

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Abstract

The invention belongs to the technical field of breeding environment improvement and purification based on probiotics and relates to a novel environmental modifier for livestock housing as well as a preparation method and an application of the novel environmental modifier. According to the preparation method, first-grade shake-flask seeds are prepared from Enterococcus faecium WEI-19, Lactobacillus plantarum WEI-12 and Bacillus coagulans WEI-125 and cultured in a seed tank and a fermentation tank, and the livestock housing environmental modifier containing thalli and fermentation products is obtained. When the environmental modifier for the livestock housing is sprayed to excrement and air of the livestock housing, ammonia gas content of the livestock housing can be reduced by 80%, contentof pathogenic bacteria in excrement is reduced by 90%, content of pathogenic bacteria in air is reduced by 70%, infection probability of bacterial livestock diseases is reduced, and an excellent growth environment is provided for the livestock.

Description

Technical field: [0001] The invention belongs to the technical field of improving and purifying breeding environment by probiotics, and relates to a novel livestock and poultry house environment improver, and a preparation method and application thereof. Background technique: [0002] In the modern large-scale livestock and poultry breeding process, a large amount of dust, harmful gases and pathogenic bacteria will be generated in the pen, which not only affects the quality of livestock and poultry, but also harms the health of the keepers. Traditional ventilation methods cannot fundamentally solve ammonia pollution and cannot effectively inhibit pathogenic bacteria. Although spraying chemical disinfectants can quickly eliminate ammonia and kill pathogenic bacteria, it is easy to cause secondary pollution and also affect livestock and poultry itself. Cause certain toxicity. [0003] Probiotic culture solution is a new and effective green environment improver. The lactic acid and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P1/00B01D53/84B01D53/58C12R1/46C12R1/25C12R1/07
CPCA01N63/00A01N63/10B01D53/58B01D53/84B01D2251/95B01D2257/91C12N1/20A01N2300/00Y02A40/70Y02A50/20
Inventor 乔琳姚宏明于遵星高长斌冯媛媛刘蕊
Owner BEIJING DAWEIJIA BIOTECH SHARE CO LTD
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