Composite microbial bacterial agent for controlling soil pollution and its preparation method and application

A technology of compound microbial inoculum and culture, applied in the field of microbial inoculum and its preparation, can solve the problems of aggravating the deterioration of soil alkalinity and accelerating the decomposition of organic matter, and achieve the effect of safe and effective degradation and inhibiting the formation of harmful spores

Active Publication Date: 2021-03-23
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the application of chemical methods, such as chemical modifiers, surfactants or biological improvement measures, increases the environmental capacity of the soil and enhances the ability of soil purification to apply lime, alkaline phosphate, iron oxide, carbonate and Chemical modifiers such as sulfides can accelerate the decomposition of organic matter; however, due to the different soil acidity and alkalinity in my country, especially in coastal plains, estuary deltas, and Huanghuaihai plains, coastal saline areas and yellow rivers are formed due to the comprehensive influence of natural conditions and soil intrinsic factors. In the saline area of ​​the Huaihai Plain, when the commonly used chemical methods are used to treat the polluted soil, the rich calcium, excessive application of lime and irrigation of alkaline sewage will aggravate the deterioration of the soil alkaline condition

Method used

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  • Composite microbial bacterial agent for controlling soil pollution and its preparation method and application
  • Composite microbial bacterial agent for controlling soil pollution and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Raw material components: 30 parts of culture of Acetobacter aceti ATCC 8303, 35 parts of culture of Fusarium cerevisiae ATCC 60881, 15 parts of culture of Bacillus sarcina NBRC 105378, 10 parts of culture of Bacillus subtilis ATCC 21556, culture of yeast NYNU14719 10 parts, 2 parts of Streptomyces brown ATCC 23896 culture, 2 parts of Trichoderma viride ATCC 26802 culture, 2 parts of Penicillium citrus ATCC 48955 culture, adsorbent (solute is fiber powder, carbon powder, kaolin, solvent is water ) 420 copies.

[0028] Preparation and application: (1) Expansion culture: each strain in the raw material components is expanded by shake flask or solid medium to obtain the culture of each strain; Sterilize for 1 hour, place and cool to normal temperature, and culture the shake flasks of various strains. The conditions for the shake flask culture are: inoculate the other strains in the raw material components except Fusarium graminearum respectively in the shake flasks, Cultiv...

Embodiment 2

[0037] Raw material components: 35 parts of culture of Acetobacter aceti ATCC 23747, 50 parts of culture of Fusarium cerevisiae ATCC 60881, 18 parts of culture of Bacillus sarcina NBRC 105378, 15 parts of culture of Bacillus subtilis ATCC 21556, culture of yeast NYNU14719 15 parts, 3 parts of Streptomyces brown ATCC 23896 culture, 3 parts of Trichoderma viride ATCC 26802 culture, 3 parts of Penicillium citrus ATCC 48955 culture and adsorbent (solute is fiber powder, carbon powder, solvent is water) 710 share.

[0038] Preparation and application: (1) Expansion culture: each strain in the raw material components is expanded by shake flask or solid medium to obtain the culture of each strain; Sterilize for 0.75 hours, place and cool to normal temperature, and culture the shake flasks of various strains. The conditions for the shake flask culture are: inoculate the other strains in the raw material components except Fusarium graminearum respectively in the shake flasks, Cultivat...

Embodiment 3

[0047] Raw material components: 45 parts of culture of Acetobacter aceti ATCC 23751, 60 parts of culture of Fusarium cerevisiae ATCC 200362, 20 parts of culture of Bacillus sarcina NBRC 105378, 20 parts of culture of Bacillus subtilis ATCC 21556, yeast NYNU 14719 20 parts of culture, 5 parts of culture of Streptomyces cellulose ATCC 3313, 5 parts of culture of Aspergillus niger AS 3.3289, 5 parts of culture of Penicillium oxalicum CGMCC No.9092 and adsorbent (solute is fiber powder, carbon powder, kaolin And anionic polyacrylamide, the solvent is water) 1100 parts.

[0048] Preparation and application: (1) Expansion culture: each strain in the raw material components is expanded by shake flask or solid medium respectively to obtain the culture of each strain; Sterilize for 1 hour, place and cool to normal temperature, and culture the shake flasks of various strains. The conditions for the shake flask culture are: inoculate the other strains in the raw material components excep...

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Abstract

The invention discloses a compound microbial agent. The compound microbial agent comprises, by weight, 30-45 parts of acetobacter aceti culture, 35-60 parts of fusarium graminearum culture, 10-20 parts of yeast culture, 15-20 parts of micrococcus culture and 10-20 parts of bacillus subtilis culture; according to the composite microbial agent, through the acetobacter aceti culture, the fusarium graminearum culture, the mixed culture of micrococcus and bacillus subtilis and other mixed microbial agents prepared by mixing other microorganisms used for treating contaminated soil, safe and effective decomposition of contaminant petroleum hydrocarbons in alkaline soil is achieved, and at the same time, the mixed culture of strains and the preparation of culture media effectively inhibit the formation of harmful spores in fusarium graminearum.

Description

technical field [0001] The invention relates to a microbial bacterial agent and its preparation method and application, in particular to a composite microbial bacterial agent for treating alkaline soil polluted by organic matter and its preparation method and application. Background technique [0002] Pollutants produced by human activities enter the soil and accumulate to a certain extent, causing soil quality to deteriorate, and then causing some indicators in crops to exceed national standards, resulting in soil pollution. There are various ways for pollutants to enter the soil. Pollutants contained in exhaust gas, especially particulate matter, settle to the ground and enter the soil under the action of gravity. Wastewater carries a large amount of pollutants into the soil. Pollutants in solid waste directly enter the soil or Its exudate enters the soil. Among them, the pollution and omission accidents in the process of oil mining, smelting, use and transportation, as w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14B09C1/10C12R1/77C12R1/125C12R1/265C12R1/02C12R1/72C12R1/465C12R1/885C12R1/80C12R1/685
CPCB09C1/105C12N1/14C12N1/16C12N1/20
Inventor 谢悦波姚竣耀刘才群
Owner 江苏世邦生物工程科技有限公司
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