Composite microbial bacterial agent for controlling soil pollution and its preparation method and application
A technology of compound microbial inoculum and culture, applied in the field of microbial inoculum and its preparation, can solve the problems of aggravating the deterioration of soil alkalinity and accelerating the decomposition of organic matter, and achieve the effect of safe and effective degradation and inhibiting the formation of harmful spores
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Embodiment 1
[0027] Raw material components: 30 parts of culture of Acetobacter aceti ATCC 8303, 35 parts of culture of Fusarium cerevisiae ATCC 60881, 15 parts of culture of Bacillus sarcina NBRC 105378, 10 parts of culture of Bacillus subtilis ATCC 21556, culture of yeast NYNU14719 10 parts, 2 parts of Streptomyces brown ATCC 23896 culture, 2 parts of Trichoderma viride ATCC 26802 culture, 2 parts of Penicillium citrus ATCC 48955 culture, adsorbent (solute is fiber powder, carbon powder, kaolin, solvent is water ) 420 copies.
[0028] Preparation and application: (1) Expansion culture: each strain in the raw material components is expanded by shake flask or solid medium to obtain the culture of each strain; Sterilize for 1 hour, place and cool to normal temperature, and culture the shake flasks of various strains. The conditions for the shake flask culture are: inoculate the other strains in the raw material components except Fusarium graminearum respectively in the shake flasks, Cultiv...
Embodiment 2
[0037] Raw material components: 35 parts of culture of Acetobacter aceti ATCC 23747, 50 parts of culture of Fusarium cerevisiae ATCC 60881, 18 parts of culture of Bacillus sarcina NBRC 105378, 15 parts of culture of Bacillus subtilis ATCC 21556, culture of yeast NYNU14719 15 parts, 3 parts of Streptomyces brown ATCC 23896 culture, 3 parts of Trichoderma viride ATCC 26802 culture, 3 parts of Penicillium citrus ATCC 48955 culture and adsorbent (solute is fiber powder, carbon powder, solvent is water) 710 share.
[0038] Preparation and application: (1) Expansion culture: each strain in the raw material components is expanded by shake flask or solid medium to obtain the culture of each strain; Sterilize for 0.75 hours, place and cool to normal temperature, and culture the shake flasks of various strains. The conditions for the shake flask culture are: inoculate the other strains in the raw material components except Fusarium graminearum respectively in the shake flasks, Cultivat...
Embodiment 3
[0047] Raw material components: 45 parts of culture of Acetobacter aceti ATCC 23751, 60 parts of culture of Fusarium cerevisiae ATCC 200362, 20 parts of culture of Bacillus sarcina NBRC 105378, 20 parts of culture of Bacillus subtilis ATCC 21556, yeast NYNU 14719 20 parts of culture, 5 parts of culture of Streptomyces cellulose ATCC 3313, 5 parts of culture of Aspergillus niger AS 3.3289, 5 parts of culture of Penicillium oxalicum CGMCC No.9092 and adsorbent (solute is fiber powder, carbon powder, kaolin And anionic polyacrylamide, the solvent is water) 1100 parts.
[0048] Preparation and application: (1) Expansion culture: each strain in the raw material components is expanded by shake flask or solid medium respectively to obtain the culture of each strain; Sterilize for 1 hour, place and cool to normal temperature, and culture the shake flasks of various strains. The conditions for the shake flask culture are: inoculate the other strains in the raw material components excep...
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Abstract
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Application Information
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