Preparation method of mushroom bran bio-fertilizer
A technology of bio-fertilizer and fungus chaff, applied in the field of bio-fertilizer preparation, can solve the problems of no zinc-rich fungus chaff method, and achieve the effects of sustainable development, thorough fermentation, and enhanced fermentation effect
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Embodiment 1
[0017] Embodiment 1: the preparation of bacterium chaff
[0018] (1) Substrate: 60% sawdust, 30% sawdust, 3% rice bran, 7% bran; pH is 5.8, water content is 65%, and zinc sulfate powder is mixed into the bacteria bag to make the bacteria bag per kilogram Zinc-rich bacterial packs containing 1000mg of zinc sulfate.
[0019] (2) Sterilization: 100°C, 90 minutes-110°C, 90 minutes-121°C, 150 minutes-simmer for 30 minutes-exhaust steam.
[0020] (3) Inoculation: use forced cooling in the cooling room to inoculate the material temperature below 18°C, and inoculate manually and seal with a manual sealing machine.
[0021] (4) Cultivation: After inoculation, it is sent to the bacteria room for cultivation.
[0022] (5) Acquisition of fungus chaff: After the growth cycle of the mushroom is completely over (about 10 months), zinc that has not been completely absorbed by the edible fungi will remain in the substrate, which is the fungus chaff.
Embodiment 2
[0023] Embodiment 2: the preparation of fermented bacterium bran
[0024] (1) Dried, crushed and sieved the fungus chaff;
[0025] (2) get the urea of 1% of bacterial chaff weight, urea is dissolved in water and make urea water, urea water is mixed with bacterial chaff, and the water content in the bacterial fertilizer after mixing is 65%;
[0026] (3) place the bacterium chaff prepared in step (2) naturally for 24 hours;
[0027] (4) Take the flocculent rice bran, mix it with the bacterial bran evenly to obtain the mixed bacterial bran; the weight of the flocculent rice bran is 5% of the weight of the bacterial bran after the treatment in step (3);
[0028] (5) The pH value of the mixed bacterial fertilizer is adjusted to 9;
[0029] (6) Put the mixed fungus chaff in a light-shielding, air-permeable closed container for natural fermentation. When the fermentation temperature reaches a high temperature of 62°C, after the high temperature lasts for 36 hours, the first stirr...
Embodiment 3
[0031] Embodiment 3: the preparation of bacterial agent
[0032] (1) Configuration of broth medium: beef extract 3g, peptone 10g, agar 20g, tap water 1000mL. Adjust the pH to 7.2 and sterilize in an autoclave at 121°C. After sterilizing, pour it into a Petri dish to make a flat plate.
[0033] (2) Separation and purification of strains
[0034] Weigh 10 g of fermented bacterium bran prepared in Example 2 in 90 mL of sterile water, and heat it in a water bath at 80° C. for 5 min. The gradient is 10 -1 , diluting it 10-fold to 10 -6 , take 10 -4 、10 -5 、10 -6 Each 0.1ml was coated on the prepared medium, and each gradient was coated with 3 plates. After coating, incubate for 48 hours in a constant temperature incubator at 37°C. take 10 -5 Microscopic examination was carried out on the microorganisms in the gradient, and they were picked out and drawn on the inclined surface to be cultivated and preserved for later use. Microscopic examination found that various types of...
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