Paenibacillus strain T1-1 with phosphorus-dissolving effect and application thereof

A technology of bacillus and strains, applied in the field of microorganisms, can solve the problems of single strain, short shelf life, and single function, and achieve good results

Active Publication Date: 2018-05-15
HUBEI FORBON TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, outstanding problems such as single strain, single function and short shelf life still need to be solved.

Method used

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  • Paenibacillus strain T1-1 with phosphorus-dissolving effect and application thereof
  • Paenibacillus strain T1-1 with phosphorus-dissolving effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 microbial bacterial agent

[0032] Inoculate the Paenibacillus strain T1-1 into the liquid medium, cultivate in a 30°C incubator, and stop the cultivation after 85% of the bacteria produce spores to obtain a fermentation broth or a culture fluid.

[0033] Wherein, the formula of described liquid culture medium is: 10g / L glucose, 5.0g / L Ca 3 (PO 4 ) 2 , 5.0g / L MgCl 2 , 0.25g / L MgSO 4 ·7H 2 O, 0.2g / L KCl, 1g / L (NH 4 ) 2 SO 4 , pH 7.0. Sterilize at 121°C for 25min.

[0034] In the obtained fermentation broth, the colony concentration was 5×10 8 CFU / mL.

Embodiment 2

[0035] Determination of Phosphorus Solubilizing Ability in Liquid Medium in Embodiment 2

[0036] Pour 100mL NBRIP liquid medium (10g / L glucose, 5.0g / L MgCl 2 , 0.25g / L MgSO 4 ·7H 2 O, 0.2g / L KCl, 1g / L (NH 4 ) 2 SO 4 , pH 7.0), add 5.0g / L inorganic phosphorus Ca respectively 3 (PO 4 ) 2 、FePO 4 , AlPO 4 And 2.0 mL / L organic phosphorus (lecithin) as a phosphorus source.

[0037] The fermented liquid obtained in Example 1 was inoculated, and Escherichia coli JM109 was inoculated as a control, and cultured on a shaking table (28° C., 160 r / min) for 8 days. Centrifuge (10 000 r / min) at 4°C for 15 minutes, take an appropriate amount of supernatant, and measure the phosphorus content (determined by molybdenum antimony colorimetric method) and pH value.

[0038]

[0039] The results showed that Paenibacillus strain T1-1 could dissolve different inorganic phosphorus and organic phosphorus, and had a positive effect on Ca 3 (PO 4 ) 2 Phosphorus dissolving ability is ve...

Embodiment 3

[0040] Example 3 Determination of Phosphorus Dissolving Ability in Solid Medium

[0041]The phosphorus-dissolving ability of Paenibacillus phospholyticum T1-1 was determined by NBRIP solid medium. Activate the strain of Paenibacillus phospholyticum T1-1 in LD solid medium and culture overnight at 30°C; use a sterile toothpick to remove the activated single colony 3 (PO 4 ) 2 The center of the NBRIP solid medium was prick inoculated, then incubated at 30 °C for 7 days, and the experiments were performed in triplicate. The colony diameter (d) and phosphate-dissolving circle diameter (D) around the colony were measured.

[0042] Phosphorus Solubilization Index (PSI): PSI=D / d.

[0043]

[0044] The results showed that Paenibacillus strain T1-1 contained Ca 3 (PO 4 ) 2 The PSI of the solid NBRIP medium is 2.46.

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Abstract

The invention belongs to the technical field of microorganisms and particularly discloses a paenibacillus strain T1-1 with a phosphorus-dissolving effect and application thereof. The preservation number of the paenibacillus strain T1-1 is CGMCC No.14927. A microbial agent or microbial fertilizer prepared by the paenibacillus strain has the advantages that the growth of vegetables can be significantly promoted, and the use amount of chemical phosphatic fertilizer can be effectively reduced; the paenibacillus strain T1-1 can be widely popularized and used in agricultural production.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Paenibacillus strain capable of dissolving phosphorus and its application. Background technique [0002] Phosphate-dissolving bacteria can secrete organic acids and phosphatases. Organic acids can convert mineral phosphorus into soluble dihydrogen phosphate and hydrogen phosphate ions. This process is called the degradation of mineral phosphorus; phosphatases promote the mineralization of phosphorus (hydrolysis ) are efficiently utilized by plants. It can be seen that lysobacteria play an important role in promoting plant phosphorus uptake. Using microbial approaches to improve the availability of phosphorus in soil can increase the nutrient level of soil soluble phosphorus, promote the effective use of phosphorus by plants, and improve the soil microbial environment. [0003] Paenibacillus has the characteristics of long survival period and strong stress resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C02F3/34C02F101/30C02F101/10C12R1/01
CPCC02F3/34C02F2101/105C02F2101/30C05G3/00C12N1/20C12N1/205C12R2001/01
Inventor 陈三凤李永斌
Owner HUBEI FORBON TECH
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