Paenibacillus strain T1-1 with phosphorus-dissolving effect and application thereof
A technology of bacillus and strains, applied in the field of microorganisms, can solve the problems of single strain, short shelf life, and single function, and achieve good results
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Embodiment 1
[0031] The preparation of embodiment 1 microbial bacterial agent
[0032] Inoculate the Paenibacillus strain T1-1 into the liquid medium, cultivate in a 30°C incubator, and stop the cultivation after 85% of the bacteria produce spores to obtain a fermentation broth or a culture fluid.
[0033] Wherein, the formula of described liquid culture medium is: 10g / L glucose, 5.0g / L Ca 3 (PO 4 ) 2 , 5.0g / L MgCl 2 , 0.25g / L MgSO 4 ·7H 2 O, 0.2g / L KCl, 1g / L (NH 4 ) 2 SO 4 , pH 7.0. Sterilize at 121°C for 25min.
[0034] In the obtained fermentation broth, the colony concentration was 5×10 8 CFU / mL.
Embodiment 2
[0035] Determination of Phosphorus Solubilizing Ability in Liquid Medium in Embodiment 2
[0036] Pour 100mL NBRIP liquid medium (10g / L glucose, 5.0g / L MgCl 2 , 0.25g / L MgSO 4 ·7H 2 O, 0.2g / L KCl, 1g / L (NH 4 ) 2 SO 4 , pH 7.0), add 5.0g / L inorganic phosphorus Ca respectively 3 (PO 4 ) 2 、FePO 4 , AlPO 4 And 2.0 mL / L organic phosphorus (lecithin) as a phosphorus source.
[0037] The fermented liquid obtained in Example 1 was inoculated, and Escherichia coli JM109 was inoculated as a control, and cultured on a shaking table (28° C., 160 r / min) for 8 days. Centrifuge (10 000 r / min) at 4°C for 15 minutes, take an appropriate amount of supernatant, and measure the phosphorus content (determined by molybdenum antimony colorimetric method) and pH value.
[0038]
[0039] The results showed that Paenibacillus strain T1-1 could dissolve different inorganic phosphorus and organic phosphorus, and had a positive effect on Ca 3 (PO 4 ) 2 Phosphorus dissolving ability is ve...
Embodiment 3
[0040] Example 3 Determination of Phosphorus Dissolving Ability in Solid Medium
[0041]The phosphorus-dissolving ability of Paenibacillus phospholyticum T1-1 was determined by NBRIP solid medium. Activate the strain of Paenibacillus phospholyticum T1-1 in LD solid medium and culture overnight at 30°C; use a sterile toothpick to remove the activated single colony 3 (PO 4 ) 2 The center of the NBRIP solid medium was prick inoculated, then incubated at 30 °C for 7 days, and the experiments were performed in triplicate. The colony diameter (d) and phosphate-dissolving circle diameter (D) around the colony were measured.
[0042] Phosphorus Solubilization Index (PSI): PSI=D / d.
[0043]
[0044] The results showed that Paenibacillus strain T1-1 contained Ca 3 (PO 4 ) 2 The PSI of the solid NBRIP medium is 2.46.
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