Preparation method of collagen-based electrospinning fiber antibacterial agent carriers
A technology of electrospun fibers and antibacterial agents, which is applied in the field of polymer carrier preparation, can solve the problems of short release time of antibacterial agents, etc., and achieve stable and sufficient release effects
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[0040] Example 1
[0041] (1) Dissolve sulfate lignin and antibacterial agent gemifloxacin in tetrahydrofuran at a mass ratio of 10:1 to prepare an organic solution with a sulfate lignin concentration of 10mg / ml, and pass the resulting organic solution through a 0.22μm Filter membrane to remove insoluble materials; slowly add the filtrate to ultrapure water under stirring at a stirring speed of 300r / min, the volume ratio of water to lignin solution is 4:1, stir for 10min, and the liquid at 5000r / min Centrifuge for 10 minutes, take the supernatant and place it in a 12-14KDa dialysis bag for 2 days for dialysis. After freeze-drying, uniform antimicrobial agent carrier particles are obtained. The microscopic appearance is shown figure 1 .
[0042] (2) The carrier particles prepared in step (1) are uniformly dispersed in deionized water using an ultrasonic cell pulverizer at a concentration of 10 mg / ml. Under 600W power, ultrasonic for 1h, the polyvinyl alcohol (PVA) with a degree of p...
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[0050] Example 2-5
[0051] Except for the lignin concentration in step (1), the other process parameters are the same as in Example 1. The lignin addition ratio in Examples 2-5 is shown in Table 1.
[0052] Table 1 Example 2-5 Lignin concentration
[0053]
[0054] Example 2-5 Antibacterial agent release and antibacterial experiments are respectively Figure 4 with 5 .
[0055] The comparison shows that with the increase of drug-loaded lignin nanoparticles, the release rate of antibacterial agents gradually increases, but there is no sudden release phenomenon for these types; the diameter of the inhibition zone increases with the increase of the content of gemifloxacin, that is, antibacterial The effect is increased.
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[0056] Example 6-8
[0057] Except for the PVA concentration in step (2), the other process parameters are the same as in Example 1. The mass-to-volume ratio of PVA in Examples 6-8 is shown in Table 2.
[0058]
[0059] The morphology of the fiber membrane prepared in Examples 6-8 is the same as that in Example 1, and the bacteriostasis, degradation performance, and antibacterial agent release performance are all good.
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