A kind of method for industrialized production of hemp protein oligopeptide

A technology of hemp protein and hemp seed is applied in the field of industrialized production of hemp protein oligopeptide, which can solve the problems of easily destroying the nutrients of hemp seed, poor color and luster of finished oligopeptides, and few protein oligopeptides. , to achieve the effect of accelerating the mass transfer rate and solubility, shortening the degreasing time, and protecting the activity

Active Publication Date: 2021-02-12
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few studies on the extraction of protein oligopeptides from hemp seeds, and the existing extraction methods are complex and easy to destroy the nutritional components of hemp seeds, or the obtained oligopeptides have poor color and odor. It cannot meet the needs of today's consumer groups. Therefore, in order to make full use of resources, it is of great significance to use a suitable production process to produce hemp protein oligopeptides

Method used

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  • A kind of method for industrialized production of hemp protein oligopeptide
  • A kind of method for industrialized production of hemp protein oligopeptide
  • A kind of method for industrialized production of hemp protein oligopeptide

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Embodiment 1

[0031] A method for industrialized production of hemp protein oligopeptides, comprising the following steps:

[0032] S1: Crush hemp seeds and pass through a 10-mesh sieve, place them in an extraction tank, use subcritical extraction method for low-temperature degreasing, add butane to the extraction tank for 25 minutes according to the mass ratio of solid to liquid at 1:9, and repeat the extraction After 2 times, take out and obtain hemp seed powder after defatting; wherein, the operating conditions of the extraction tank are that the temperature is 20°C and the pressure is 0.03MPa;

[0033] S2: Add distilled water to the hemp seed powder after degreasing as described in S1 according to the material-to-liquid ratio of 1:8, and then put it in a colloid mill for grinding for 10 minutes to obtain a mixed solution;

[0034] S3 Alkaline Extraction: Add an acid-base regulator to the mixture described in S2, adjust the pH to 8.5, and put it in an ultrasonic-assisted extraction with ...

Embodiment 2

[0041] A method for industrialized production of hemp protein oligopeptides, comprising the following steps:

[0042] S1: Crush hemp seeds and pass through a 60-mesh sieve, place them in an extraction tank, use subcritical extraction method for low-temperature degreasing, add butane to the extraction tank for 30 minutes according to the mass ratio of solid to liquid at 1:10, and repeat the extraction After 2 times, take out and obtain hemp seed powder after defatting; wherein, the operating conditions of the extraction tank are that the temperature is 25°C and the pressure is 0.05MPa;

[0043] S2: Add distilled water to the hemp seed powder after degreasing as described in S1 according to the material-to-liquid ratio of 1:12, and then put it in a colloid mill for grinding for 15 minutes to obtain a mixed solution;

[0044] S3 Alkaline Extraction: Add an acid-base regulator to the mixed solution described in S2, adjust the pH value to 9.5, and put it in an ultrasonic-assisted e...

Embodiment 3

[0051] A method for industrialized production of hemp protein oligopeptides, comprising the following steps:

[0052] S1: Crush hemp seeds and pass through a 40-mesh sieve, place them in an extraction tank, use subcritical extraction method for low-temperature degreasing, add butane to the extraction tank for 28 minutes according to the mass ratio of solid to liquid at 1:9.5, and repeat the extraction After 2 times, take out and obtain hemp seed powder after defatting; wherein, the operating conditions of the extraction tank are that the temperature is 23°C and the pressure is 0.04MPa;

[0053] S2: add distilled water to the hemp seed powder after degreasing as described in S1 according to the material-to-liquid ratio of 1:10, and then put it in a colloid mill for grinding for 12 minutes to obtain a mixed solution;

[0054] S3 Alkaline Extraction: Add an acid-base regulator to the mixed solution described in S2, adjust the pH value to 9, and put it in an ultrasonic-assisted ex...

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Abstract

The invention provides an industrial production method of hemp protein oligopeptide and belongs to the field of plant extraction. Hemp seeds are subjected as a raw material to the steps of low temperature defatting, pulping, alkali extraction, acid precipitation, enzymatic hydrolysis, centrifuging, microfiltration, ultrafiltration, concentration, low temperature drying, and finishing. The hemp protein oligopeptide is prepared by using enzymes that relieve bitter and fishy smell, controlling enzymatic hydrolysis conditions, performing low temperature operation in the whole process, and using the means of ultrafiltration and the like; the prepared hemp protein oligopeptide has little bitter and fishy smell, has light yellow color and good quality, easily dissolves, has high stability, is simple to operate and suitable for industrial production, has the functions such as good activity of lowering blood pressure in vitro and in vivo, can adjust human health, and has a promising developmentand application prospect in food industry, health foods, medicine and other fields.

Description

【Technical field】 [0001] The invention relates to the field of plant extraction, in particular to a method for industrialized production of hemp protein oligopeptides. [0002] 【technical background】 [0003] Hemp seed is the dry mature seed of Moraceae hemp, another name is hemp seed, hemp, harvested when the autumn fruit matures, removes impurities, and becomes hemp seed after drying. Hemp seed is sweet in taste and flat in nature, and belongs to the spleen, stomach, and large intestine meridians. It has the effects of moistening the intestines and laxatives, moisturizing dryness and killing insects. Hemp seed is rich in protein, fatty oil, lecithin, glucuronic acid, sterols, calcium, magnesium, vitamins B1 and B2, fatty oil contains oleic acid, linoleic acid, linolenic acid and cannabinol and other components, hemp seed The most important feature of hemp seed is that it simultaneously provides essential fatty acids (EFAs) and α-linolenic acid necessary in the human diet; ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34C07K1/36A23L33/18A61K38/01A61P9/12
CPCA23V2002/00A61K38/011A23L33/18C07K1/145C07K1/34C07K1/36C12P21/06B01D11/0288
Inventor 卫萍游向荣张雅媛孙健谢小强李明娟周葵王颖黄承祖
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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