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Phenol antioxidant content measuring method with carbon quantum dots being fluorescence indicator

A technology of phenolic antioxidants and fluorescent indicators, which can be used in fluorescence/phosphorescence, measuring devices, and material analysis through optical means. It can solve the problems of expensive instruments and high reagent requirements, achieve simple synthesis methods, and improve detection sensitivity. , The effect of reducing the cost of raw materials

Inactive Publication Date: 2018-05-29
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiencies in the prior art, the present invention provides a method for measuring the content of phenolic antioxidants using carbon quantum dots as a fluorescent indicator, which solves the problems of expensive instruments and high requirements for reagents in the existing detection methods

Method used

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  • Phenol antioxidant content measuring method with carbon quantum dots being fluorescence indicator
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of carbon quantum dots using Enteromorpha as raw material

[0023] First wash Enteromorpha with tap water, remove impurities such as surface silt, then rinse with pure water; take by weighing 200g of Enteromorpha raw material (dry weight about 10g) after washing, according to the mass ratio of Enteromorpha and pure water is 2 Mix Enteromorpha and pure water in a ratio of 1, put it into the raw material container of a tissue masher, pulverize for 5min, and obtain Enteromorpha slurry; take 10g of Enteromorpha slurry, put it into a 30mL polytetrafluorothermal reaction kettle and Add a cover and put it into an outsourcing casing to seal it; put the reactor containing Enteromorpha slurry into a microwave reactor, set the temperature to 200°C, and the pressure to 20atm, react for 60min, and then stop heating; wait until the temperature of the reactor is lowered to Below 40°C, open the lid of the reaction kettle, the reaction product is a reddish-brown solution...

Embodiment 2

[0034] (1) Preparation of carbon quantum dots with citric acid and ethylenediamine as raw materials

[0035] Weigh 1.00 g of citric acid and 300 μL of ethylenediamine, mix and dilute to 10 mL with ultrapure water. Move the mixed solution into a hydrothermal reactor, react at 180°C for 6 hours, take out the reactant after the reactor is cooled to room temperature, purify by ultrapure water dialysis, and freeze-dry to obtain fluorescent carbon quantum dot powder;

[0036] (2) 100 mM hydrogen peroxide, 1000 μg / mL carbon quantum dots and 100 μg / mL horseradish peroxidase solutions were respectively prepared using TRIS-HCl solution with pH=7;

[0037] (3) Quickly mix the three solutions in step (2) (final concentration: hydrogen peroxide 10.0mM, carbon quantum dots 25g / mL and horseradish peroxidase solution 5μg / mL), and let stand for 5min;

[0038] (4) Measure the fluorescence spectrum of the mixed solution obtained in step (3) with a spectrofluorometer, and record the fluorescence...

Embodiment 3

[0046] (1) Preparation of carbon quantum dots from carrots

[0047] First, the carrots are washed and cut into pieces for later use; 200g carrots are weighed, and the carrots and pure water are mixed according to the mass ratio of carrots and pure water in a ratio of 4:1, put into the raw material container of a tissue masher, and pulverized for 5 minutes to obtain Carrot slurry; Weigh 10g carrot slurry, put it into a polytetrafluorohydrothermal reaction kettle and add a cover and put it into an outsourcing sleeve for sealing; put the reaction kettle with carrot slurry into a microwave reactor, and set the temperature at 180 ℃, the pressure is 20atm, react for 60min, then stop heating; wait for the reactor to cool down to room temperature, open the lid of the reactor, the reaction product is a mixture of yellow solid and liquid; collect the reaction product mixture in a centrifuge tube, centrifuge at 8000rpm for 20min, Transfer the supernatant of the centrifuge tube to a clean...

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Abstract

The invention discloses a phenol antioxidant content measuring method with carbon quantum dots being a fluorescence indicator. Though the feature that fluorescence of the carbon quantum dots can be quenched by a benzoquinone material which is obtained from a reaction of a phenol antioxidant and reactive oxide free radicals, on the condition that the reactive oxide free radicals are excessive, thefluorescence quenching degree is in direction proportion to the concentration of a phenolic compound within a certain concentration range, and thereby, the concentration of phenolic substances can besensitively detected. The indicator carbon quantum dot synthesis method is simple, particularly, enteromorpha serves as the raw material of the carbon quantum dots, the cost of the raw material is further lowered, the fluorescence yield of the product is also high, and the detecting sensitivity is improved; the detecting method can be used for detecting the content of multiple phenolic antioxidants, the capability of capturing free radicals by different types of phenolic antioxidants can also be subjected to transverse evaluation, and the phenol antioxidant content measuring method has high value in the field of development of functional foods.

Description

technical field [0001] The invention relates to a method for detecting the content of phenolic antioxidants using the fluorescence characteristics of carbon quantum dots, in particular to the fact that the fluorescence of carbon quantum dots can be quenched by benzoquinones generated by the reaction of phenolic antioxidants and active oxygen free radicals, Under the condition of excessive active oxygen free radicals, the degree of fluorescence quenching is proportional to the concentration of phenolic compounds within a certain concentration range, thus the concentration of phenolic compounds can be detected sensitively. The invention belongs to the technical application field of functional nanometer materials. Background technique [0002] Studies have shown that once the balance between the production and elimination of free radicals in the body is broken, oxidative damage will occur, leading to aging, pigment formation, vascular disease, cell cancer, diabetes and diabetic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428G01N2021/6417G01N2021/6432G01N2021/6439
Inventor 王晓娟陈奇黄方葛保胜刘真真
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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