Phenol antioxidant content measuring method with carbon quantum dots being fluorescence indicator
A technology of phenolic antioxidants and fluorescent indicators, which can be used in fluorescence/phosphorescence, measuring devices, and material analysis through optical means. It can solve the problems of expensive instruments and high reagent requirements, achieve simple synthesis methods, and improve detection sensitivity. , The effect of reducing the cost of raw materials
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Embodiment 1
[0022] (1) Preparation of carbon quantum dots using Enteromorpha as raw material
[0023] First wash Enteromorpha with tap water, remove impurities such as surface silt, then rinse with pure water; take by weighing 200g of Enteromorpha raw material (dry weight about 10g) after washing, according to the mass ratio of Enteromorpha and pure water is 2 Mix Enteromorpha and pure water in a ratio of 1, put it into the raw material container of a tissue masher, pulverize for 5min, and obtain Enteromorpha slurry; take 10g of Enteromorpha slurry, put it into a 30mL polytetrafluorothermal reaction kettle and Add a cover and put it into an outsourcing casing to seal it; put the reactor containing Enteromorpha slurry into a microwave reactor, set the temperature to 200°C, and the pressure to 20atm, react for 60min, and then stop heating; wait until the temperature of the reactor is lowered to Below 40°C, open the lid of the reaction kettle, the reaction product is a reddish-brown solution...
Embodiment 2
[0034] (1) Preparation of carbon quantum dots with citric acid and ethylenediamine as raw materials
[0035] Weigh 1.00 g of citric acid and 300 μL of ethylenediamine, mix and dilute to 10 mL with ultrapure water. Move the mixed solution into a hydrothermal reactor, react at 180°C for 6 hours, take out the reactant after the reactor is cooled to room temperature, purify by ultrapure water dialysis, and freeze-dry to obtain fluorescent carbon quantum dot powder;
[0036] (2) 100 mM hydrogen peroxide, 1000 μg / mL carbon quantum dots and 100 μg / mL horseradish peroxidase solutions were respectively prepared using TRIS-HCl solution with pH=7;
[0037] (3) Quickly mix the three solutions in step (2) (final concentration: hydrogen peroxide 10.0mM, carbon quantum dots 25g / mL and horseradish peroxidase solution 5μg / mL), and let stand for 5min;
[0038] (4) Measure the fluorescence spectrum of the mixed solution obtained in step (3) with a spectrofluorometer, and record the fluorescence...
Embodiment 3
[0046] (1) Preparation of carbon quantum dots from carrots
[0047] First, the carrots are washed and cut into pieces for later use; 200g carrots are weighed, and the carrots and pure water are mixed according to the mass ratio of carrots and pure water in a ratio of 4:1, put into the raw material container of a tissue masher, and pulverized for 5 minutes to obtain Carrot slurry; Weigh 10g carrot slurry, put it into a polytetrafluorohydrothermal reaction kettle and add a cover and put it into an outsourcing sleeve for sealing; put the reaction kettle with carrot slurry into a microwave reactor, and set the temperature at 180 ℃, the pressure is 20atm, react for 60min, then stop heating; wait for the reactor to cool down to room temperature, open the lid of the reactor, the reaction product is a mixture of yellow solid and liquid; collect the reaction product mixture in a centrifuge tube, centrifuge at 8000rpm for 20min, Transfer the supernatant of the centrifuge tube to a clean...
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