Preservation fixation solution of pathological tissue and preparation method of preservation fixation solution

Abstract

The invention belongs to the technical field of specimen treatment and particularly relates to a preservation fixation solution of a pathological tissue. The preservation fixation solution of the pathological tissue comprises the following ingredients in parts by mass: 10-15 parts of furfuraldehyde, 8-12 parts of hydroxyl compound, 1-6 parts of mannitol extract, 6-10 parts of alliin, 6-8 parts of(A2) trimethylglycine, 1-3 parts of carbon-based-4,4-diacetone-xylose-fatty alcohol-phosphate chelation compound polymer and 50-60 parts of water. The preservation fixation solution is free from methanal and alcohol, and nontoxic, is not required to be replaced, has no influence on a working environment, a good specimen preservation effect and a significant antibacterial effect; the tissue is freefrom mildewing and shrinkage, natural in color, flexible and wet; at the same time, the solution is stable in property; the specimen has no mildew spots or rancidness after simulated accelerated preservation for 6 months at 40 DEG C; the preservation solution is clear; the specimen keeps initial shape, color, rigidity and tenacity; and the solution can be massively popularized and used, particularly in a high-temperature area.

Description

technical field [0001] The invention belongs to the technical field of specimen processing, and in particular relates to a pathological tissue preservation fixative. Background technique [0002] Biopsy pathological diagnosis is the first diagnosis in surgery and is the gold standard. Doctors can make a correct diagnosis based on the test results of the specimens and formulate the next treatment plan for the disease. The accuracy of pathology reports plays a decisive role in the diagnosis and treatment of diseases to a considerable extent. Correct handling of pathological specimens can not only provide an effective guarantee for pathological diagnosis and clinical diagnosis, but also maintain the patient's body and mind, and prevent disputes caused by improper specimen handling. Existing surveys have shown that the current specimen handling methods in the operating room cannot effectively prevent nursing errors. [0003] The most critical steps in the processing of pathol...

AI Technical Summary

Problems solved by technology

However, the long-term use of ethanol-containing preservation solution will easily cause the specimen to lose water, condense and deform. This may be due to the small molecular weight of ethanol, which will penetrate into the interior of the specimen after long-term use.

At the same time, due to the volatility of ethanol, the preservation solution will gradually become invalid, especially in hot areas, the preservation solution is difficult to promote

[0006] Chinese patent CN1047061C discloses an antiseptic preservation solution for corpse specimens. The components of the preservation solution are formulated from benzoate, ethanol, potassium nitrate and glycerol. Although the preservation solution has reliable antiseptic performance, it is safe and non-toxic to use , the advantage of no bad irritating smell, but the preservation solution has no fixation effect on the tissues of human specimens, it is easy to cause the shape of the tissues to deform, and it does not serve the purpose of preserving the original shape of the specimens, which is not conducive to the development of research

However, after 6 months of simulated accelerated storage at 40°C, the specimens appear cloudy and begin to deform. It is not suitable for high temperature and long-term storage.