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Glucose oxidase mutant with improved heat resistance

A technology of glucose oxidase and mutants, which is applied in the direction of oxidoreductase, enzyme, and microbial-based methods, can solve the problems of poor thermal stability, application limitations, and increased equipment investment of glucose oxidase, so as to be beneficial to wide application, Improved heat resistance and broad market prospects

Active Publication Date: 2018-06-05
QINGDAO VLAND BIOTECH GRP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the thermal stability of glucose oxidase derived from Aspergillus niger is poor, and its aqueous solution is kept at 65°C for 5 minutes, and the remaining enzyme activity is less than 40%, which limits the application of this enzyme in pellet feed
At present, the method of spraying glucose oxidase liquid onto the feed after feed granulation not only increases equipment investment, but also cannot guarantee the stability of the enzyme preparation and the uniformity of the distribution of the enzyme preparation in the feed

Method used

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  • Glucose oxidase mutant with improved heat resistance

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Experimental program
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Effect test

Embodiment 1

[0056] Example 1 Obtainment of glucose oxidase heat-resistant single point mutant

[0057] 1.1 Amplification of the glucose oxidase gene

[0058] Aspergillus niger ( Aspergillus niger ) genome as a template for PCR amplification, PCR primers GOD-F1 and GOD-R1 are as follows:

[0059] GOD-F1: GGTATTGAGGCATCTTTGTTGAC

[0060] GOD-R1: TTATTGCATAGAAGCGTAATC

[0061] The PCR product was recovered by gel, connected to the pEASY-T vector, transformed into Escherichia coli DH5α, and the correct transformant was picked for sequencing. Sequencing results showed that the nucleotide sequence of the amplified gene fragment was SEQ ID NO: 2, and the encoded amino acid sequence was SEQ ID NO: 1. Through NCBI BLAST comparison, it was found that the sequence similarity between SEQ ID NO: 1 and the glucose oxidase from Aspergillus niger was as high as 100%, so it was confirmed that the gene obtained by PCR was the glucose oxidase gene and named GOD.

[0062] Amplification and Synthesis of ...

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Abstract

The invention relates to the field of gene modification, and specifically provides a glucose oxidase mutant with improved heat resistance. According o the present invention, the heat resistance of theglucose oxidase mutant is substantially improved compared to wild type glucose oxidase, the residual enzyme activity of the glucose oxidase mutant is increased by 11.3-66.9% after treatment for 10 min at a temperature of 60 DEGC, and the residual enzyme activity of the glucose oxidase mutant is increased by 23.7-105.5% after treatment for 5 min at a temperature of 65 DEGC, such that the glucose oxidase mutant can be widely used in feeds so as to provide wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of genetic modification, and in particular relates to a glucose oxidase mutant with improved heat resistance. Background technique [0002] Glucose oxidase is an aerobic dehydrogenase that specifically oxidizes β-D-glucose to generate gluconic acid and hydrogen peroxide. Glucose oxidase usually forms a redox system with catalase, oxidizes β-D-glucose in the presence of molecular oxygen to generate D-gluconolactone, and consumes oxygen to generate hydrogen peroxide. Catalase decomposes hydrogen peroxide to generate water and 1 / 2 oxygen, and then the water combines with gluconolactone to produce gluconic acid. Glucose oxidase shows strong specificity to β-D-glucopyranose. The hydroxyl group on glucose molecule C1 is crucial to the catalytic activity of the enzyme, and the hydroxyl group at the β position is 160 times higher than that at the α position. Glucose oxidase is completely inactive on L-glucose and ...

Claims

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Application Information

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IPC IPC(8): C12N9/04C12N15/53C12N15/81C12N1/19A23K20/189C12R1/84
CPCC12N9/0006C12Y101/03004
Inventor 吴秀秀邵弨
Owner QINGDAO VLAND BIOTECH GRP
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