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Preparation method of ovalbumin nano particle loaded with EGCG (Epigallocatechin Gallate), and application thereof

A technology of ovalbumin and nanoparticles, which is applied in the field of preparation of EGCG-loaded ovalbumin nanoparticles, which can solve the problems of poor stability, easy oxidation, and decomposition, and achieve the effects of reducing oxidative decomposition, easy cell phagocytosis, and increasing half-life

Inactive Publication Date: 2018-06-08
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The EGCG used in the present invention is the main component of the biologically active substance catechin extracted from tea leaves, but natural active substances also have unavoidable defects. It decomposes under strong or acidic conditions, so it is necessary to research and develop excellent drug delivery systems

Method used

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  • Preparation method of ovalbumin nano particle loaded with EGCG (Epigallocatechin Gallate), and application thereof
  • Preparation method of ovalbumin nano particle loaded with EGCG (Epigallocatechin Gallate), and application thereof
  • Preparation method of ovalbumin nano particle loaded with EGCG (Epigallocatechin Gallate), and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 is the preparation method of loading EGCG ovalbumin nanoparticles, comprising the following steps:

[0034] (1) Weigh ovalbumin (OVA) into a 50 mL centrifuge tube, then add PBS buffer solution with pH 6.6, vortex to fully dissolve, and hydrate overnight;

[0035] (2) Centrifuge the ovalbumin solution hydrated overnight and collect the supernatant to remove insoluble impurities;

[0036] (3) Keep the solution obtained in step (2) in a water bath at 80°C for 20 min;

[0037] (4) After the solution in step (3) is bathed in water, add EGCG to the solution immediately, and the solution becomes an emulsion at this time;

[0038] (5) Vortex the EGCG-containing emulsion obtained in step (4) for 30 s, then immediately cool to room temperature with running water;

[0039] (6) Immediately put the emulsion obtained in step (5) into an ice bath, and sonicate the probe for 1 min;

[0040] (7) Centrifuge the emulsion obtained in step (6) at a low speed and collect the ...

Embodiment 2

[0044] Experiment 1: The morphology of nanoparticles was observed by field emission scanning electron microscopy. First, suspend the lyophilized nanoparticles in secondary water or PBS buffer, and dilute to an appropriate concentration, then drop the suspension onto a silicon wafer and air-dry it. Before analysis, the nanoparticles need to be vacuum-sprayed with platinum.

[0045] figure 1 Field emission scanning electron microscope image of nanoparticles. It can be seen from the obtained electron microscope photos that the nanoparticles are spherical, the particle size distribution is relatively uniform, and they are densely packed, which is also the characteristic of protein nanoparticles.

[0046] Test 2: In order to simulate the in vivo release of the drug, the release solution uses phosphate buffer solution with pH = 7.4 and pH = 6.8 to simulate the pH in the body. First, accurately weigh an appropriate amount of nanoparticles (the mass of the nanoparticles is calculate...

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Abstract

The invention discloses a preparation method of an ovalbumin (OVA) nano particle loaded with epigallocatechin gallate (EGCG), and application thereof. The method comprises the steps of firstly preparing the ovalbumin nano particle successfully loaded with the EGCG through a heating self-assembling method; then representing multiple physicochemical properties. The nano particle has narrower particle size distribution, a higher drug loading capacity (40+ / -3 mug / mg) and a favorable drug controlled release effect (50 percent is released after 24 hours). According to the preparation method providedby the invention, the EGCG with a natural anti-inflammatory performance and the ovalbumin are self-assembled to form the nano particle, so that the oxidation of the EGCG before arriving at a focus part is reduced, a pharmacokinetics characteristic of the EGCG is improved, a half-life period in vivo is improved, and a treatment effect of the EGCG on ulcerative colitis is improved.

Description

technical field [0001] The invention belongs to the technical field of nano drug carriers, and in particular relates to a preparation method, characterization and application of EGCG-loaded ovalbumin nanoparticles. Background technique [0002] Ulcerative colitis (UC) is the most important type of inflammatory bowel disease, and its etiology may include environmental, infection, immune and genetic factors. People who carry certain genes may be more susceptible to UC. According to data, the incidence of ulcerative colitis is relatively high in European and American countries. Taking the United States as an example, the incidence rate is as high as 2‰. Symptoms of UC usually get worse over time. With the improvement of people's living standards, the number of cases of UC reported in China has also increased significantly in recent years. According to the statistics of cases in many hospitals, the prevalence of ulcerative colitis is 11.6 / 100,000, and the actual cases may be m...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K31/353A61K47/42A61P1/04
Inventor 肖波苟双全陈秋冰汪敏
Owner SOUTHWEST UNIVERSITY
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