A kind of acellular allogeneic dermal matrix and its application in oral diseases

A dermal matrix, decellularization technology, applied in the field of tissue engineering of biomedical materials, can solve the problems affecting wound healing and function recovery, function and shape impact, large surgical trauma, etc., to reduce cytotoxicity and rejection. Immune rejection, no contouring effect

Active Publication Date: 2021-03-30
BEIJING JAYYALIFE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Oral mucosal defect repair mainly uses autologous tissue repair such as locally adjacent transferred mucosal flap, buccal fat pad flap, free skin graft, etc. Although it is easy to survive, there are still some defects: the function and shape of the autologous donor area and recipient area After healing, it will lead to obvious postoperative scar deformity; second-stage repair, surgical trauma is large, and transplanted tissue needs to be cut from other parts of the body, and often causes dysfunction and complications in the donor site to varying degrees, causing negative effects such as pain and course of the patient. influences
However, fixation with glutaraldehyde and / or formaldehyde, due to the cytotoxicity of glutaraldehyde or formaldehyde, leads to the fact that the biological tissue cross-linked using them affects the growth of the recipient's normal tissue to a certain extent after implantation in the human body , which in turn affects the healing of the wound and the recovery of function; the alkaline solution used to decellularize the tissue, especially sodium hydroxide or potassium hydroxide is a strong base, will cause the collagen in the acellular allogeneic dermal matrix to be in alkaline Hydrolysis under conditions

Method used

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  • A kind of acellular allogeneic dermal matrix and its application in oral diseases
  • A kind of acellular allogeneic dermal matrix and its application in oral diseases
  • A kind of acellular allogeneic dermal matrix and its application in oral diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation method of acellular allodermal matrix

[0051] Put allogeneic leather raw materials into 0.3g / L phospholipase and 0.1g / L trypsin solution, the pH value of the enzyme solution is 7.0, the temperature is 37°C, shake at 100rpm for 2 hours, replace the enzyme solution once, repeat this process once, The semi-finished product A is obtained; the phospholipase is a mixture of phospholipase A1: phospholipase A2: phospholipase C: phospholipase D according to the mass ratio of 1:1:1:1.

[0052] The semi-finished product A was taken out, put into a container filled with physiological saline solution, soaked in physiological saline, oscillated 3 times, each time for 2 hours, the oscillation speed was 80 rpm, and the temperature was 2°C to obtain the semi-finished product B.

[0053] Put the semi-finished product B into the solution containing 0.2g / LTritonX-100, 50KHz, 220W, ultrasonic for 5 minutes, soak for 3 hours, repeat this process 3 times, and get the se...

Embodiment 2

[0059] Example 2 Preparation method of acellular allogeneic dermal matrix

[0060] Put allogeneic leather raw materials into 0.2g / L phospholipase and 0.2g / L trypsin solution, the pH value of the enzyme solution is 7.0, the temperature is 37°C, shake at 100rpm for 2 hours, replace the enzyme solution once, repeat this process once, The semi-finished product A is obtained; the phospholipase is a mixture of phospholipase A1, phospholipase A2, phospholipase C and phospholipase D according to the mass ratio of 1:1:1:1.

[0061] The semi-finished product A was taken out, put into a container filled with physiological saline solution, soaked in physiological saline, oscillated 3 times, each time for 2 hours, the oscillation speed was 80 rpm, and the temperature was 2°C to obtain the semi-finished product B.

[0062] Put the semi-finished product B into a solution containing 0.2g / LSDS, 10mM EDTA, 50KHz, 220W, ultrasonic for 7 minutes, soak for 2 hours, repeat this process 3 times, and...

experiment example 1

[0070] Experimental example 1 Acellular allogeneic dermal matrix (oral patch) used in gingival defect surgery

[0071] Case 1

[0072] Li, male, 59 years old, had gingival defects in the left upper incisor and right upper incisor. The largest defect area was 1.0cm×0.8cm, and the smallest was 0.2cm×0.1cm. The gingival flap was performed under local anesthesia, and the surgical wound was completely hemostasis. Take the acellular allogeneic dermal matrix prepared in Example 1, which is slightly larger than the defect surface, soak it in normal saline and wash it, cut 2 small incisions in the center to facilitate drainage, attach the rough surface to the wound surface, and suture intermittently between the edge of the patch and the wound edge. Stay long. After the suture is completed, use the long thread left to wrap the iodoform oil gauze back and pressurize it, so that the patch is closely attached to the wound. On the 10th day after the operation, the iodoform bag was opened,...

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Abstract

The invention relates to application of an allograft acellular dermal matrix in oral diseases, in particular to application of the allograft acellular dermal matrix in the repairing of cleft lip and / or alveolar cleft, cleft palate and oral mucous membrane defect, the repairing of periodontal defect and gingival recession, the repairing of nasal mucosa, tooth implantation, the repairing of furcation involvement and the prevention of complications such as dry socket after impacted tooth extraction, and belongs to the technical field of biological medical material tissue engineering. The allograft acellular dermal matrix is a product improved by a process, the mechanical performance of the product is suitable for surgery, and the sewing strength of the product is 16-18N; the allograft acellular dermal matrix is free of immunological rejection, capable of fast inducing tissue regeneration, soft in texture after implantation, free of contour feeling and good in in-vivo compatibility and hasa stable stent and template effect.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biomedical materials, and in particular relates to an acellular allogeneic dermal matrix for the repair of cleft lip and / alveolar protrusion, cleft palate, oral mucosal defect repair, gingival defect, gingival recession repair, nasal mucosa repair, and dental implantation. Application in the prevention of complications such as surgery, furcation lesion repair, and dry socket after impacted tooth extraction. Background technique [0002] Oral mucosal defect repair mainly uses autologous tissue repair such as locally adjacent transferred mucosal flap, buccal fat pad flap, free skin graft, etc. Although it is easy to survive, there are still some defects: the function and shape of the autologous donor area and recipient area After healing, it will lead to obvious postoperative scar deformity; second-stage repair, surgical trauma is large, and transplanted tissue needs to be cut from oth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/54
CPCA61L27/362A61L27/3633A61L27/3687A61L27/3691A61L27/50A61L27/54A61L2300/412A61L2430/12A61L2430/40
Inventor 孙继煌石清东
Owner BEIJING JAYYALIFE BIOTECH CO LTD
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