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Radix panacis quinquefolii controlreference extract as well as preparation method and application thereof

A technology of American ginseng extract and American ginseng, applied in the field of American ginseng reference extract and its preparation

Inactive Publication Date: 2018-06-12
广州科曼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because of the above-mentioned specific requirements for the reference extract of traditional Chinese medicine, there is no or immature American ginseng reference extract product prepared by using American ginseng medicinal materials as raw materials in the existing technology

Method used

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  • Radix panacis quinquefolii controlreference extract as well as preparation method and application thereof
  • Radix panacis quinquefolii controlreference extract as well as preparation method and application thereof
  • Radix panacis quinquefolii controlreference extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1: Preparation of American ginseng reference extract

[0081] The present embodiment provides the preparation method of American ginseng reference extract of the present invention, and its method flowchart is as follows figure 1 shown.

[0082] One: extract

[0083] Select American ginseng medicinal materials (Canada, authentic medicinal materials) and make them into powders. The medicinal materials powders are passed through a No. 2 sieve (850±29 μm, 24 meshes), and then add ethanol with a volume 10 times its mass (that is, the solid-liquid ratio is 1:10 (w / V)), after flash extraction for 1 min, filter with medium-speed qualitative filter paper, collect filter residue 1 and filtrate 1, filter residue 1 is extracted twice in the same way, and combine the filtrate collected for the second extraction with filtrate 1 to obtain the extract , the extract is evaporated to dryness to obtain a dry paste;

[0084] Two: Preparation of American ginseng extract

[0...

Embodiment 2

[0096] The character analysis of embodiment 2 American ginseng control extract

[0097] 1. Apparent state: the American ginseng reference extract obtained in Example 1 is yellow gray powder.

[0098] 2. Moisture determination: according to the 2015 edition Chinese Pharmacopoeia Appendix IX G (reduced pressure drying method). The test result shows that the water content of the control extract of American ginseng is 3.91%.

[0099] 3. Consistency test: 3 batches of American ginseng reference extracts were prepared according to the method in Example 1, and the thinness of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd between each batch was measured. The layer chromatography detection result difference is very little, and the fluorescent spots of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd all have obvious display, and distribution is very consistent; Through high performance liquid chromatography detec...

Embodiment 3

[0108] This example provides the application of the American ginseng control extract prepared in Example 1.

[0109] Commercial medicinal materials were analyzed by thin-layer chromatography and high-performance liquid chromatography with chemical reference substance and American ginseng reference extract as reference substances.

[0110] 1 TLC

[0111] 1.1 Sample preparation

[0112] Chemical reference solution: Precisely weigh ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc, and ginsenoside Rd, respectively, and prepare 0.2 mg / ml solutions with methanol.

[0113] American ginseng control extract solution: densely weigh the American ginseng extract reference substance, add methanol for ultrasonic extraction for 15 minutes, constant volume, prepare a solution with a concentration of 3 mg / ml, filter the membrane, and take the filtrate as the extract reference substance solution.

[0114] Medicinal material test solution: Accurately weigh 1g of American ginsen...

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Abstract

The invention provides a radix panacis quinquefolii controlreference extract which is prepared by the following steps: repeatedly extracting different batches of radix panacis quinquefolii medicinal material powder to obtain different batches of radix panacis quinquefolii extracts; then, blending the different batches of radix panacis quinquefolii extracts to obtain the radix panacis quinquefoliicontrolreference extract. Since the radix panacis quinquefolii controlreference extract provided by the invention is prepared by blending different batches of extracts, consistency of different batches of radix panacis quinquefolii controlreference extracts is guaranteed; moreover, the radix panacis quinquefolii controlreference extract has stable and uniform characteristics and is convenient to use. The radix panacis quinquefolii controlreference extract serves as a referencecontrol in the quality control of radix panacis quinquefolii and a medicinal material containing radix panacis quinquefolii / radix panacis quinquefolii active ingredients or traditional Chinese medicine preparations; in the quality control process, the radix panacis quinquefolii controlreference extract can be directlyused after simple treatment, and thus is easy to operate; the radix panacis quinquefolii controlreference extract can be applied to qualitative identification of the medicinal material or the traditional Chinese medicine preparation as well as semi-quantitative and even quantitative analysis.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a control extract of American ginseng and its preparation method and application. Background technique [0002] American ginseng (scientific name: Panax quinquefolius) is a perennial herbaceous plant belonging to the Araliaceae genus. Its main component is ginsenoside, and the five kinds of saponins that have been isolated are: ginsenoside R0, Rb1, Rg1, Re and pseudo-ginsenoside F11. [0003] The current quality control mode of traditional Chinese medicine is basically following the development of natural medicinal chemistry, with the idea of ​​analysis methods targeting one or several active ingredients of traditional Chinese medicine and quality standards that can be both qualitative and quantitative, referring to the quality of foreign herbal medicines. The control method draws on the model of chemical drug quality control, and establi...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02
CPCG01N30/90G01N30/02
Inventor 郭隆钢陆顺瑶许舜军谢培山许铮弟张奕尧张和灿
Owner 广州科曼生物科技有限公司
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