Method for improving quality of tobacco leaves by lysobacter sp

A kind of technology of lysobacteria and tobacco leaves, applied in the field of tobacco alcoholization and fermentation

Inactive Publication Date: 2018-06-19
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention isolates a lysobacterium strain from nature for the first time, which has been id...

Method used

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  • Method for improving quality of tobacco leaves by lysobacter sp
  • Method for improving quality of tobacco leaves by lysobacter sp
  • Method for improving quality of tobacco leaves by lysobacter sp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] ——The application of Lysobacterium C8-1 in improving the quality of tobacco leaves, the specific steps are as follows:

[0024] (1) Slant culture: medium: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; g; agar 15g; distilled water 1000mL, pH7.2; culture temperature is 30°C, culture time is 7d.

[0025] (2) Seed culture: pick part of the mycelium from the slope and put it into the seed liquid, and shake the flask for 36 hours. Seed medium: dextrin 120g; soybean powder 40g; yeast extract 2g; tryptophan 0.5g; β-alanine 5g; magnesium sulfate 0.5g; ammonium phosphate 0.2g; ℃.

[0026] (3) Fermentation culture: Inoculate 10% of the inoculum into the fermentation medium, and culture in shake flasks for 6 days. Medium: soybean powder 10g; glucose 10g; peptone 3g; sodium chloride 2.5g; calcium carbonate 2g;

[0027] (4) Take 1L of the fermentation culture solution, centrifuge at 3500r / min for 10min, rem...

Embodiment 2

[0044] Repeat Example 1, with the following differences: the treatment concentration is 20 mL per 1 kg of shredded tobacco sprayed with bacterial dose.

[0045] ——Chemical Composition Determination Results

[0046] The test results of the chemical components of the tobacco leaves are as follows (see Tables 4 and 5 for details). The experimental results show that, from the perspective of a single compound, the internal chemical components of the tobacco leaves treated with the bacterial agent have changed significantly. Compared with the control, the content of total sugar and reducing sugar increased greatly, and the content of total nitrogen, cellulose, protein and nicotine decreased compared with the control. Sugar-to-nitrogen ratio, sugar-to-base ratio, and nitrogen-to-base ratio all improved. The systematic test data shows that all kinds of shredded tobacco treated with bacterial agents are better than the control, and the components in the shredded tobacco tend to be bal...

Embodiment 3

[0057] Repeat Example 1, with the following differences: the treatment concentration is 30 mL per 1 kg of shredded tobacco sprayed with bacterial dose.

[0058] ——Chemical Composition Determination Results

[0059] The test results of the chemical components of the tobacco leaves are as follows (see Tables 7 and 8 for details). The experimental results show that, from the perspective of a single compound, the internal chemical components of the tobacco leaves treated with the bacterial agent have changed significantly. Compared with the control, the content of total sugar and reducing sugar increased greatly, and the content of total nitrogen, cellulose, protein and nicotine decreased compared with the control. Sugar-to-nitrogen ratio, sugar-to-base ratio, and nitrogen-to-base ratio all improved. The systematic test data shows that all kinds of shredded tobacco treated with bacterial agents are better than the control, and the components in the shredded tobacco tend to be bal...

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Abstract

The invention discloses a method for improving the quality of tobacco leaves by lysobacter sp. The strain is classified and named as Lysobacter sp C8-1, and is preservatized at the General Microbiology Center of the China Microbial Culture Collection Management Committee on September 28, 2017, and the preservation number is CGMCC No. 1.16271. After microbiological analysis and 16S rDNA gene sequence analysis, it is indicated that the strain is a new type of bacteria. After being sprayed with C8-1 bacteria agent and fermented with alcoholization, the quality of tobacco leaves is improved, the availability of tobacco leaves is greatly improved, the fermentation of tobacco leaves is accelerated, the grade quality of tobacco leaves is improved, the aroma quality and reducing sugar content of tobacco leaves are increased, the content of protein, cellulose, total nitrogen and nicotine is decreased, and the ratio of sugar to nitrogen, the ratio of sugar to nicotine and the ratio of nitrogen to nicotine are increased, thereby making the chemical compositions in the tobacco leaves more harmonious and improving the sensory quality.

Description

technical field [0001] The invention belongs to the technical field of tobacco alcoholization and fermentation, and in particular relates to a method for improving the quality of tobacco leaves by using a newly discovered lysobacterium. Background technique [0002] Tobacco is one of the important pillars of our country's finances and has made great contributions to our country's social and economic development. In order to promote the progress of tobacco in our country and realize the sustainable development of the cigarette industry, we must take the road of tobacco technology innovation. The quality of tobacco leaves directly affects the intrinsic taste of cigarettes. The State Tobacco Monopoly Administration clearly pointed out that it is necessary to "overcome the key technologies that affect the aroma, quality and aroma of tobacco leaves" and narrow the quality gap with foreign tobacco leaves in order to improve domestic and foreign market competitiveness. [0003] T...

Claims

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Application Information

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IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 李源栋张翼鹏刘秀明者为郭青陈兴杜刚李文均
Owner CHINA TOBACCO YUNNAN IND
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