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Method for degrading benzidine by using Oriental Variegated Coralbean leaf crude enzyme solution

A technology of benzidine and crude enzyme liquid, applied in chemical instruments and methods, water pollutants, water/sludge/sewage treatment, etc., can solve the problems of high production cost, cumbersome removal process, easy to cause explosion, etc. Low cost, easy availability, mild reaction conditions

Inactive Publication Date: 2018-06-19
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In summary, the problem of prior art is: use ClO 2 The production cost is higher, and the removal process is more cumbersome, and it is easy to cause an explosion; at the same time, using ClO 2 Due to the introduction of chlorine in the process of removing benzidine, hypochlorous acid can be produced, resulting in the generation of some harmful organic halogenated substances, which are difficult to effectively solve by chemical removal methods

Method used

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  • Method for degrading benzidine by using Oriental Variegated Coralbean leaf crude enzyme solution
  • Method for degrading benzidine by using Oriental Variegated Coralbean leaf crude enzyme solution
  • Method for degrading benzidine by using Oriental Variegated Coralbean leaf crude enzyme solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Effects of different concentrations of Erythrina japonica crude enzymes on the removal of benzidine

[0028] Determination of Erythrina POD (Peroxidase) Enzyme Activity by Taking Crude Enzyme from Erythrina

[0029] Take 4ml of benzidine, respectively take 10U, 5U, and 2U of enzyme activity units, and the corresponding Erythrina enzyme solution is 42μL, 26μL, 10.4μL, pH 6.0 phosphate buffer 15.942mL, 15.958mL, 15.974mL, 1mg / L H 2 o 2 16μL;

[0030] After loading 1ml of methanol into each test tube, take 1mL of each sample at the time of control (0min), 1min, 3min, 5min, 15min, 45min, 90min, 120min, and 180min, and add 8μL KAl(SO 4 ) 2, After taking it, put it in the refrigerator for 12 hours. Then centrifuge at 4°C for 10 min at 12000 r / min.

[0031] The content of benzidine was determined by liquid chromatography.

[0032] From figure 2 It can be seen that when the enzyme activity unit is 10U, the clearance rate reaches more than 90% in the first minu...

Embodiment 2

[0033] Example 2 Effects of different pH buffers on the removal of benzidine by the crude enzyme of Erythrina chinensis

[0034] Determination of enzyme activity of fresh Erythrina japonica crude enzyme

[0035] Take 4ml of benzidine, take the corresponding Erythrina enzyme solution of 5U enzyme activity units, use pH 3.0, pH 4.0, pH 5.0, pH 7.0, pH 8.0, pH 9.0 buffer and 1mg / L H 2 o 2 16 μL. Use citric acid buffer for pH 3.0, acetate buffer for pH 4.0 and 5.0, phosphate buffer for pH 6.0-8.0, and borate buffer for pH 9.0.

[0036] After loading 1ml of methanol into each test tube, take 1mL of each sample of the control (0min), 1min, 3min, 5min, 15min, 45min, 90min, 120min, and 180min, and add 8μL of KAl (SO 4 ) 2 , After taking it, put it in the refrigerator for 12 hours. Then centrifuge at 4°C for 10 min at 12000 r / min.

[0037] The content of benzidine was determined by liquid chromatography.

[0038] From image 3 It can be seen in the figure that the clearance rat...

Embodiment 3

[0039] The influence of embodiment 3 different temperatures on the removal of benzidine by Erythrina japonica crude enzyme

[0040] Determination of enzyme activity of fresh Erythrina japonica crude enzyme

[0041] Take 4ml of benzidine, take the corresponding Erythrina enzyme solution of 5U of enzyme activity unit, take the phosphate buffer solution with pH of 7.0 and 1mg / L H 2 o 2 Under the conditions, the temperature of the reaction system was changed, and the reaction system was respectively placed in a water bath with a temperature of 25°C, 30°C, 40°C, 45°C, and 50°C.

[0042] After loading 1ml of methanol into each test tube, take 1mL of the reaction mixture of a control (0min), 1min, 3min, 5min, 15min, 45min, 90min, 120min, and 180min, and add 8μL of KAl(SO 4 ) 2 , After taking it, put it in the refrigerator for 12 hours. Then centrifuge at 4°C for 10 min at 12000 r / min.

[0043] The content of benzidine was determined by liquid chromatography.

[0044] From Fig...

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PUM

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Abstract

The invention belongs to the technical field of organic pollution treatment, and discloses a method for degrading benzidine by using an Oriental Variegated Coralbean leaf crude enzyme solution. The method comprises the following steps: weighing 5 g of fresh and mature Oriental Variegated Coralbean leaves, removing main veins, cutting the leaves into pieces, grinding the cut leaves by using 25 ml of a phosphoric acid buffer solution with the pH value being 6.8 to extract peroxidase, centrifuging the obtained solution, taking the obtained supernatant which is the crude enzyme solution, and refrigerating the supernatant in a refrigerator; determining the enzyme activity through adopting a guaiacol technology: adding 0.1 ml of the enzyme solution to start a reaction, and recording the OD valuechange at 30 DEG C under a wavelength of 470 nm; and degrading the benzidine by using the Oriental Variegated Coralbean leaf peroxidase. The method overcomes the disadvantages of complicated operation, severe reaction conditions and poor purification ability of a ClO2 oxidation technology, and has the advantages of greenness, environmental protection, high efficiency and broad industrial prospect. Oriental Variegated Coralbean is widely planted as a landscape plant, is rich in resources, and can be easily obtained in people 's daily life, so the method has the characteristics of low cost, greenness, safety and no pollution.

Description

technical field [0001] The invention belongs to the technical field of organic pollutant treatment, and in particular relates to a method for degrading benzidine by using the crude enzyme liquid of erythrina twig leaves. Background technique [0002] Benzidine is a white or slightly yellowish stable needle-like crystal or powder, flammable, and its color deepens when it is exposed to light in the air, and commercially available products are often light yellow. Insoluble in cold water, slightly soluble in hot water and ether, easily soluble in acetic acid and dilute hydrochloric acid. Benzidine is a dibasic weak base. Generally, the salts of benzidine are commonly used, and its sulfate is often used in industry, while hydrochloride and acetate are mostly used in laboratories. The chemical properties of benzidine are similar to that of aniline, and it can react with oxidizing substances to generate derivatives of quinoid structure; it can also undergo diazotization reaction w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/38
CPCC02F3/342C02F2101/38
Inventor 肖强周秀梅吴素林王楠蒲圩王雨雪
Owner HUBEI UNIV FOR NATITIES
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